Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,327,165 A→T 19.0% intergenic (‑155/+121) wcaK ← / ← wzxC predicted pyruvyl transferase/colanic acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,327,1650AT19.0% 94.5 / 16.5 42intergenic (‑155/+121)wcaK/wzxCpredicted pyruvyl transferase/colanic acid exporter
Reads supporting (aligned to +/- strand):  ref base A (11/23);  new base T (2/6);  total (13/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGAT  >  minE/1327098‑1327233
                                                                   |                                                                    
tCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTg                                                                   >  1:4129314/1‑71 (MQ=255)
     ccTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGa                                                              <  1:1270555/71‑1 (MQ=255)
      cTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGAt                                                             >  1:2301945/1‑71 (MQ=255)
       tACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCa                                                                      >  1:2859225/1‑61 (MQ=255)
          aaGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGa                                                         <  1:23414/71‑1 (MQ=255)
                      caaaaCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGt                                              >  1:1589576/4‑70 (MQ=255)
                        gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCtt                                           <  1:3948198/71‑1 (MQ=255)
                        accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCtt                                           <  1:347533/68‑1 (MQ=11)
                        accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCt                                            >  1:895719/4‑70 (MQ=11)
                          cccGTAGGACGTATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt                                         <  1:2099847/70‑1 (MQ=11)
                          cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTa                                          <  1:2000872/69‑1 (MQ=11)
                          cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt                                         <  1:2093025/70‑1 (MQ=11)
                          aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt                                         <  1:4782157/71‑1 (MQ=255)
                          aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt                                         <  1:3576398/71‑1 (MQ=255)
                           ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc                                        <  1:1226928/71‑1 (MQ=11)
                           ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc                                        <  1:1220100/71‑1 (MQ=11)
                            cGTAGTACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc                                        <  1:1832529/70‑1 (MQ=255)
                            cGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCa                                       >  1:3286212/1‑71 (MQ=11)
                            cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa                                       <  1:5316478/71‑1 (MQ=255)
                            cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa                                       <  1:2638605/71‑1 (MQ=255)
                             gTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGc                                                                  >  1:1305785/1‑43 (MQ=38)
                             ccaGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                                      >  1:5340866/3‑71 (MQ=9)
                              tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAgg                                     >  1:4931444/1‑71 (MQ=255)
                              tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg                                      <  1:3563313/70‑1 (MQ=255)
                              caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                                                     <  1:4084969/54‑1 (MQ=2)
                              caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                                      <  1:950276/69‑1 (MQ=14)
                              caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                                      <  1:1941816/69‑1 (MQ=14)
                                ggACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGc                                    <  1:3405046/70‑1 (MQ=255)
                                  aCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc                                                <  1:5170376/56‑1 (MQ=9)
                                        aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc                           >  1:3192198/1‑71 (MQ=255)
                                        aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc                           >  1:3858079/1‑71 (MQ=255)
                                              ttCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa                                       <  1:5477899/53‑1 (MQ=255)
                                                cACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg                                      >  1:3742831/1‑52 (MQ=255)
                                                     cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTccc                         <  1:2828115/60‑1 (MQ=255)
                                                     cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAAc              <  1:3956110/71‑1 (MQ=255)
                                                       cATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCg            <  1:4400215/71‑1 (MQ=255)
                                                         tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc                                        <  1:478267/41‑1 (MQ=255)
                                                         tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc                                        <  1:4169605/41‑1 (MQ=255)
                                                           cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGaa               >  1:1200941/1‑64 (MQ=255)
                                                           cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg         >  1:2889671/1‑70 (MQ=255)
                                                                ttCAGTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg         <  1:1815605/65‑1 (MQ=255)
                                                                ttCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGca                    <  1:1959955/54‑1 (MQ=255)
                                                                  cAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGAt  <  1:608985/70‑1 (MQ=255)
                                                                   |                                                                    
TCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGAT  >  minE/1327098‑1327233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: