| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,395,358 | A→C | 12.8% | I22L (ATC→CTC) ‡ | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,395,358 | 0 | A | C | 12.8% | 184.9 / 24.8 | 86 | I22L (ATC→CTC) ‡ | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base A (45/30); new base C (2/9); total (47/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
CCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGA > minE/1395290‑1395428 | ccAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATc < 1:894684/71‑1 (MQ=255) cAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCg < 1:2851816/71‑1 (MQ=255) cAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCg < 1:3157539/71‑1 (MQ=255) atgcatAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGc < 1:5144516/71‑1 (MQ=255) catAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCtt > 1:2210088/1‑71 (MQ=255) catAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCt < 1:5021084/70‑1 (MQ=255) tAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATc < 1:2138289/61‑1 (MQ=255) tAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtc > 1:5070636/1‑71 (MQ=255) tAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtc > 1:685511/1‑71 (MQ=255) aaCTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtc > 1:4036094/1‑70 (MQ=255) aCTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtct > 1:2071719/1‑70 (MQ=255) ccccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtctc < 1:228408/67‑1 (MQ=255) cccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGtt > 1:3771341/1‑57 (MQ=255) cccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:2579060/71‑1 (MQ=255) ccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGcc < 1:5264894/59‑1 (MQ=255) ccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAc > 1:2706281/1‑67 (MQ=255) ccGCAGTCTCCAGCGCGAAATCGTATGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg > 1:635835/1‑70 (MQ=255) cGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:5225024/1‑71 (MQ=255) gCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:5306107/1‑71 (MQ=255) gCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:660005/1‑71 (MQ=255) gCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGGTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:1389234/1‑71 (MQ=255) gTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATc < 1:931724/48‑1 (MQ=255) tctcCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCg < 1:1807780/48‑1 (MQ=255) ctcCAGCGCGAAATCGTTTGACCGGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCgg > 1:3612387/1‑71 (MQ=255) tcCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCg < 1:968773/61‑1 (MQ=255) tcCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCggg > 1:4405991/1‑71 (MQ=255) cAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGt > 1:2444831/1‑45 (MQ=255) cAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg > 1:4176297/1‑71 (MQ=255) cAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg > 1:729124/1‑71 (MQ=255) gcgcGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGtt < 1:2222784/44‑1 (MQ=255) gcgcGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGtt < 1:2995409/44‑1 (MQ=255) gcgcGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa > 1:4068343/1‑54 (MQ=255) gcgcGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa > 1:2890089/1‑54 (MQ=255) gcgcGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa > 1:1899379/1‑54 (MQ=255) gcgcGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa > 1:861092/1‑54 (MQ=255) cgAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:1719305/1‑71 (MQ=255) cgAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:1707370/1‑71 (MQ=255) cgAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:7046/1‑71 (MQ=255) gAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGt < 1:4810592/55‑1 (MQ=255) gAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGt < 1:2350916/55‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCttt < 1:1821564/45‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtct < 1:616655/47‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa > 1:1568340/1‑49 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCa > 1:4271485/1‑49 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAcc < 1:1375379/51‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAcc < 1:1220346/51‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAcc < 1:2395392/51‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGt < 1:3022663/54‑1 (MQ=255) aaTCGTTTGACCTGACCTCGAGGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:5423496/1‑55 (MQ=255) aaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:1540246/1‑55 (MQ=255) aTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:1561261/51‑1 (MQ=255) aTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:902423/51‑1 (MQ=255) aTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:2479554/51‑1 (MQ=255) cGTTTGCCCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAc > 1:4283757/1‑46 (MQ=255) cGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAc > 1:1054151/1‑46 (MQ=255) gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTg > 1:210461/1‑35 (MQ=255) gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:2179918/1‑71 (MQ=255) tttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTtctc < 1:2904769/42‑1 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:4508323/1‑48 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:459019/1‑48 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:3335461/1‑48 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc > 1:1240193/1‑70 (MQ=255) gacctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg < 1:3104764/50‑1 (MQ=255) tCGGCGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:5507853/59‑1 (MQ=255) tCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCaa < 1:3094563/54‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCTCTCTTCAGACTCCTACCCTAAGTa < 1:1912440/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:5461045/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:5606809/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:961379/66‑1 (MQ=255) cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg > 1:4075428/1‑45 (MQ=255) cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:570409/1‑57 (MQ=255) gACGGCGTTTATAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAttt > 1:345318/1‑71 (MQ=255) cGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTAtattc < 1:1329297/71‑2 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt < 1:5433845/68‑1 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt < 1:433957/68‑1 (MQ=255) gTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:3885576/1‑71 (MQ=255) atttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCtcg > 1:3109744/2‑69 (MQ=255) tttttACTTGTCGCCTTTCTGACGGGTATTGCGGgcg < 1:1611621/37‑1 (MQ=12) tttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCa < 1:4844851/44‑1 (MQ=16) tttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa < 1:4913978/52‑1 (MQ=255) ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:2495962/1‑71 (MQ=255) ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:5049856/1‑71 (MQ=255) tttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCtaa > 1:3363584/1‑54 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:4477010/1‑40 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:4926033/1‑70 (MQ=255) ttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGa > 1:4581485/1‑71 (MQ=255) aTCGTTGCCTTTCTCACCGGTATTGCGGGTGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:2188262/70‑1 (MQ=255) aTCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa < 1:4649244/71‑1 (MQ=255) | CCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGA > minE/1395290‑1395428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |