| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,395,399 | C→T | 19.7% | T35T (ACC→ACT) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,395,399 | 0 | C | T | 19.7% | 135.0 / 25.5 | 66 | T35T (ACC→ACT) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base T (3/10); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
GTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTT > minE/1395330‑1395464 | gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:2179918/1‑71 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc > 1:1240193/1‑70 (MQ=255) tCGGCGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:5507853/59‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCTCTCTTCAGACTCCTACCCTAAGTa < 1:1912440/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:961379/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:5461045/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:5606809/66‑1 (MQ=255) cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:570409/1‑57 (MQ=255) gACGGCGTTTATAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAttt > 1:345318/1‑71 (MQ=255) cGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTAtattc < 1:1329297/71‑2 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt < 1:5433845/68‑1 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt < 1:433957/68‑1 (MQ=255) gTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:3885576/1‑71 (MQ=255) atttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCtcg > 1:3109744/2‑69 (MQ=255) tttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa < 1:4913978/52‑1 (MQ=255) ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:5049856/1‑71 (MQ=255) ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:2495962/1‑71 (MQ=255) tttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCtaa > 1:3363584/1‑54 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:4926033/1‑70 (MQ=255) ttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGa > 1:4581485/1‑71 (MQ=255) aTCGTTGCCTTTCTCACCGGTATTGCGGGTGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:2188262/70‑1 (MQ=255) aTCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa < 1:4649244/71‑1 (MQ=255) tCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:4514188/43‑1 (MQ=255) tCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:5166761/43‑1 (MQ=255) tCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCtt < 1:5064535/62‑1 (MQ=255) ttGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa < 1:3752649/71‑1 (MQ=255) ttGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa < 1:5577743/71‑1 (MQ=255) gCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGt > 1:5173362/1‑68 (MQ=255) ccTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACAt < 1:3398826/71‑1 (MQ=255) ttCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGaa > 1:1215099/1‑62 (MQ=255) ttCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGcc > 1:796732/1‑71 (MQ=255) ttCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGcc > 1:4008181/1‑71 (MQ=255) tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGac > 1:1206445/1‑36 (MQ=255) tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTGTTACCGATGAAGTACATGccc > 1:2692158/1‑71 (MQ=255) tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGccc > 1:1987298/1‑71 (MQ=255) ctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGccc > 1:557475/1‑70 (MQ=255) aCCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTa < 1:2298029/40‑1 (MQ=255) aCCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTc < 1:4786186/70‑1 (MQ=255) tATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa < 1:2040793/51‑1 (MQ=255) tATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa < 1:5633364/51‑1 (MQ=255) aTTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTcctat < 1:4691839/68‑4 (MQ=255) aTTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTcctat < 1:5156775/68‑4 (MQ=255) aTTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTcctat < 1:1824549/68‑4 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTAttt < 1:1616264/36‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAg < 1:4564215/51‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtg < 1:4153850/71‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtg < 1:3216434/71‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTA‑CGATGAAGTAc > 1:261351/1‑53 (MQ=255) gCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:4262473/1‑40 (MQ=255) gCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:3901986/1‑40 (MQ=255) gCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtggt > 1:4023571/1‑71 (MQ=255) cGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtggtg > 1:4463386/1‑71 (MQ=255) gggCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtggtgg < 1:2389281/71‑1 (MQ=255) cgcTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAt > 1:5515954/1‑71 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:4276862/37‑1 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:3362466/37‑1 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:2538815/37‑1 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGcc > 1:4824744/1‑48 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtgg < 1:5537199/61‑1 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtgg < 1:2533110/61‑1 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttcttc > 1:2153550/1‑71 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttcttc > 1:5036779/1‑71 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttcttc > 1:459481/1‑71 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttct < 1:5132943/69‑1 (MQ=255) gCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAtt > 1:2390881/1‑66 (MQ=255) cAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACa > 1:2365508/1‑41 (MQ=255) cAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACa > 1:2636017/1‑41 (MQ=255) cAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttctt < 1:3241071/68‑1 (MQ=255) cAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTcttctt > 1:696417/1‑71 (MQ=255) cAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTcttctt > 1:2719088/1‑71 (MQ=255) aaaCCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATtcttct < 1:1268916/69‑1 (MQ=255) aCCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc > 1:4026626/1‑63 (MQ=255) cccGACACTCAGTATTTTTCTTACCGATGAAGTACa > 1:1671558/1‑36 (MQ=255) | GTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTCTT > minE/1395330‑1395464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |