Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,395,675 T→C 19.1% F127F (TTT→TTC setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,395,6750TC19.1% 192.2 / 38.7 94F127F (TTT→TTCsetBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base C (10/8);  total (52/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 115 total aligned reads displayed.

CTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCT  >  minE/1395606‑1395744
                                                                     |                                                                     
ctactTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttg                                                                      <  1:499377/71‑1 (MQ=255)
  actTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGttt                                                                       >  1:3575381/1‑68 (MQ=255)
  actTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcc                                                                    >  1:3536042/1‑71 (MQ=255)
  actTTGATTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGATCGACCGCTAACCCGCAAATGTttgcc                                                                    >  1:114151/1‑71 (MQ=255)
   ctTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcc                                                                    <  1:1871584/70‑1 (MQ=255)
      tGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttg                                                                >  1:4764043/1‑71 (MQ=255)
        ttttGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttgcc                                                              <  1:2629633/71‑1 (MQ=255)
           tGCTATTCGTTGGCGTCTTTCTTATCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCACTTGCCCGt                                                           >  1:5608040/1‑71 (MQ=255)
           tGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttg                                                                      >  1:518647/1‑60 (MQ=255)
             cTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGa                                                         <  1:3905033/71‑1 (MQ=255)
              tATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        >  1:1225743/1‑71 (MQ=255)
                ttCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        <  1:1181248/69‑1 (MQ=255)
                 tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                                     >  1:3798946/1‑71 (MQ=255)
                 tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                                     >  1:673789/1‑71 (MQ=255)
                 tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                                     >  1:3063987/1‑71 (MQ=255)
                  cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt                                                     <  1:2518552/70‑1 (MQ=255)
                  cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATg                                                    >  1:4623018/1‑71 (MQ=255)
                     tGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGcc                                                  >  1:855280/1‑70 (MQ=255)
                       gCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        <  1:2916648/62‑1 (MQ=255)
                        cGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGAc                                               >  1:528450/1‑70 (MQ=255)
                         gTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaa                                             <  1:1485890/71‑1 (MQ=255)
                          tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        <  1:101739/59‑1 (MQ=255)
                          tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        <  1:3232235/59‑1 (MQ=255)
                          tctttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        <  1:1493566/59‑1 (MQ=255)
                            tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGttt                                                                       <  1:15082/42‑1 (MQ=255)
                            tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGc                                                   <  1:5233794/62‑1 (MQ=255)
                            tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAAc                                          >  1:4570821/1‑71 (MQ=255)
                             ttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaa                                             <  1:314623/67‑1 (MQ=255)
                             ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTg                                                          <  1:520729/54‑1 (MQ=255)
                             ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTg                                                          <  1:527433/54‑1 (MQ=255)
                             ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACagaa                                           >  1:5021657/1‑69 (MQ=255)
                             ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACagaa                                           >  1:1648555/1‑69 (MQ=255)
                                  agcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGaa                                                        >  1:4240929/1‑51 (MQ=255)
                                   gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGt                                   <  1:2887441/71‑1 (MQ=255)
                                   gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACAGGACGt                                   <  1:5205782/71‑1 (MQ=255)
                                    cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATTCCGACAAAACCGGACGTg                                  <  1:3663542/71‑1 (MQ=255)
                                    cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                                  <  1:1868552/71‑1 (MQ=255)
                                      gcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAtag                                                   <  1:1653959/52‑3 (MQ=255)
                                       cTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttgc                                                               <  1:5360100/39‑1 (MQ=255)
                                       cTTTGGCTCGACCGCTAACCCGCAAATGTttgcccttgc                                                               <  1:3445092/39‑1 (MQ=255)
                                         ttGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCg                                                            >  1:1211650/1‑40 (MQ=255)
                                         ttGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAAc                                                       >  1:904067/1‑45 (MQ=255)
                                         ttGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAAc                                                       >  1:2319935/1‑45 (MQ=255)
                                          tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAAc                                                       >  1:4151377/1‑44 (MQ=255)
                                          tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc                              <  1:2859038/69‑1 (MQ=255)
                                          tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc                              <  1:4069707/69‑1 (MQ=255)
                                           ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                            <  1:2464191/70‑1 (MQ=255)
                                           ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                            <  1:3938887/70‑1 (MQ=255)
                                            gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                          <  1:2601181/71‑1 (MQ=255)
                                             cTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACg                                    <  1:1969996/60‑1 (MQ=255)
                                              tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                        >  1:1455267/1‑71 (MQ=255)
                                              tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                        >  1:4926446/1‑71 (MQ=255)
                                               cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                                  >  1:3630451/1‑60 (MQ=255)
                                               cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg                       >  1:3794624/1‑71 (MQ=255)
                                                 aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACa                                                      >  1:2103780/1‑38 (MQ=255)
                                                 aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACa                                                      >  1:319160/1‑38 (MQ=255)
                                                 aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa                                           >  1:3869454/1‑49 (MQ=255)
                                                 aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa                                           >  1:5340959/1‑49 (MQ=255)
                                                 aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa                                           >  1:5334500/1‑49 (MQ=255)
                                                 aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                     <  1:1246216/71‑1 (MQ=255)
                                                 aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                     <  1:5499068/71‑1 (MQ=255)
                                                 aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                     <  1:1754783/71‑1 (MQ=255)
                                                 aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                     <  1:1748280/71‑1 (MQ=255)
                                                   cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAggcc                                     >  1:3379320/1‑51 (MQ=255)
                                                   cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                     >  1:5679529/1‑69 (MQ=255)
                                                   cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                     >  1:4275324/1‑69 (MQ=255)
                                                    gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg                  >  1:2190858/1‑71 (MQ=255)
                                                     cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                 <  1:1596494/71‑1 (MQ=255)
                                                     cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                 >  1:449811/1‑71 (MQ=255)
                                                     cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                 >  1:3744981/1‑71 (MQ=255)
                                                      tAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaa                                            >  1:2489677/1‑43 (MQ=255)
                                                       aaCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctc               >  1:4711307/1‑71 (MQ=255)
                                                        aCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctct              >  1:2786936/1‑71 (MQ=255)
                                                        aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACg                             >  1:3234405/1‑56 (MQ=255)
                                                          ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt            <  1:4381194/71‑1 (MQ=255)
                                                          ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt            <  1:1777486/71‑1 (MQ=255)
                                                           cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt           <  1:5526964/71‑1 (MQ=255)
                                                           cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt            <  1:4016472/70‑1 (MQ=255)
                                                            gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                          <  1:4100766/55‑1 (MQ=255)
                                                            gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGa                         >  1:2115148/1‑56 (MQ=255)
                                                            gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt          <  1:2502730/71‑1 (MQ=255)
                                                            gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt          >  1:4897041/1‑71 (MQ=255)
                                                            gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt             <  1:1207736/68‑1 (MQ=255)
                                                             cAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttttt         <  1:3541979/71‑1 (MQ=255)
                                                              aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt         >  1:2876511/1‑70 (MQ=255)
                                                              aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa        >  1:2025824/1‑71 (MQ=255)
                                                               aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCg                                        <  1:4948479/38‑1 (MQ=255)
                                                                 tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCgg                                       >  1:3330730/1‑37 (MQ=255)
                                                                 tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGa                                      >  1:2205589/1‑38 (MQ=255)
                                                                 tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc                                     >  1:567505/1‑39 (MQ=255)
                                                                 tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc                                     >  1:828424/1‑39 (MQ=255)
                                                                  gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg    >  1:4945487/1‑71 (MQ=255)
                                                                  gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg    >  1:3163144/1‑71 (MQ=255)
                                                                  gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg      >  1:4327109/1‑69 (MQ=255)
                                                                   tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt          <  1:4347731/64‑1 (MQ=255)
                                                                   tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTa        <  1:661291/66‑1 (MQ=255)
                                                                    ttgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt  >  1:576392/1‑71 (MQ=255)
                                                                     tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt          >  1:686189/1‑62 (MQ=255)
                                                                     tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt             <  1:2221757/59‑1 (MQ=255)
                                                                     tgcccttgcccGTGAACATGACGACAAAACCGGACGt                                   >  1:1833024/1‑37 (MQ=255)
                                                                     |                                                                     
CTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCT  >  minE/1395606‑1395744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: