| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,395,693 | T→C | 18.8% | H133H (CAT→CAC) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,395,693 | 0 | T | C | 18.8% | 217.8 / 42.0 | 106 | H133H (CAT→CAC) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base T (43/43); new base C (13/7); total (56/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
TCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGG > minE/1395623‑1395763 | tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt > 1:3798946/1‑71 (MQ=255)tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt > 1:673789/1‑71 (MQ=255)tCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt > 1:3063987/1‑71 (MQ=255) cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAt < 1:2518552/70‑1 (MQ=255) cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATg > 1:2272248/1‑71 (MQ=255) cGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATg > 1:4623018/1‑71 (MQ=255) tGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGcc > 1:855280/1‑70 (MQ=255) cGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGAc > 1:528450/1‑70 (MQ=255) gTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaa < 1:1485890/71‑1 (MQ=255) tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGc < 1:5233794/62‑1 (MQ=255) tttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAAc > 1:4570821/1‑71 (MQ=255) ttcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaa < 1:314623/67‑1 (MQ=255) ttctgAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACagaa > 1:5021657/1‑69 (MQ=255) tcttAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAATCCg > 1:5358291/1‑71 (MQ=255) gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGt < 1:2887441/71‑1 (MQ=255) gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACAGGACGt < 1:5205782/71‑1 (MQ=255) cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg < 1:1868552/71‑1 (MQ=255) gcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACAtag < 1:1653959/52‑3 (MQ=255) tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc < 1:4069707/69‑1 (MQ=255) tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc < 1:2859038/69‑1 (MQ=255) ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg < 1:3938887/70‑1 (MQ=255) ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg < 1:2464191/70‑1 (MQ=255) gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg < 1:2601181/71‑1 (MQ=255) cTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACg < 1:1969996/60‑1 (MQ=255) tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt > 1:1455267/1‑71 (MQ=255) tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt > 1:4926446/1‑71 (MQ=255) cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg > 1:3630451/1‑60 (MQ=255) cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg > 1:3794624/1‑71 (MQ=255) aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa > 1:3869454/1‑49 (MQ=255) aCCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaaa > 1:5340959/1‑49 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:1246216/71‑1 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:1754783/71‑1 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:1748280/71‑1 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:5499068/71‑1 (MQ=255) ccGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAAca > 1:2689226/1‑49 (MQ=255) cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAggcc > 1:3379320/1‑51 (MQ=255) cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt > 1:5679529/1‑69 (MQ=255) cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt > 1:4275324/1‑69 (MQ=255) gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg > 1:2190858/1‑71 (MQ=255) cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc > 1:3744981/1‑71 (MQ=255) cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc < 1:1596494/71‑1 (MQ=255) tAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACaaa > 1:2489677/1‑43 (MQ=255) aaCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctc > 1:4711307/1‑71 (MQ=255) aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACg > 1:3234405/1‑56 (MQ=255) aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAgt < 1:3406017/68‑2 (MQ=255) ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt < 1:4381194/71‑1 (MQ=255) ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt < 1:1777486/71‑1 (MQ=255) cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt < 1:5526964/71‑1 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg < 1:4100766/55‑1 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGa > 1:2115148/1‑56 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt > 1:4897041/1‑71 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:2502730/71‑1 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt < 1:1207736/68‑1 (MQ=255) gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCAt > 1:1305554/1‑57 (MQ=255) cAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttttt < 1:3541979/71‑1 (MQ=255) aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt > 1:2876511/1‑70 (MQ=255) aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa > 1:2025824/1‑71 (MQ=255) aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCg < 1:4948479/38‑1 (MQ=255) aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAc > 1:170295/1‑71 (MQ=255) tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCgg > 1:3330730/1‑37 (MQ=255) tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc > 1:828424/1‑39 (MQ=255) tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc > 1:567505/1‑39 (MQ=255) gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg > 1:3163144/1‑71 (MQ=255) gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg > 1:4327109/1‑69 (MQ=255) tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:4347731/64‑1 (MQ=255) tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTa < 1:661291/66‑1 (MQ=255) ttgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt > 1:576392/1‑71 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGt > 1:1917951/1‑37 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGt > 1:5201057/1‑37 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt > 1:686189/1‑62 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt < 1:2221757/59‑1 (MQ=255) tgcccttgcccGTGAACATGACGACAAAACCGGACGt > 1:1833024/1‑37 (MQ=255) gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt > 1:5071142/1‑69 (MQ=255) gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc < 1:5692343/70‑1 (MQ=255) gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc < 1:3412457/70‑1 (MQ=255) cccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCa < 1:1567975/70‑1 (MQ=255) cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt > 1:444304/1‑71 (MQ=255) cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt > 1:3063026/1‑71 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt < 1:3018376/40‑1 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt < 1:4801565/40‑1 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:2090912/57‑1 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAg < 1:1125024/68‑1 (MQ=255) tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt < 1:4686250/56‑1 (MQ=255) tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa > 1:3694005/3‑59 (MQ=255) tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa > 1:839972/3‑59 (MQ=255) cgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTAc > 1:3942509/2‑59 (MQ=255) gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:800569/70‑1 (MQ=255) cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa < 1:3983690/71‑1 (MQ=255) ccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa < 1:3915962/70‑1 (MQ=255) ccGAGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:2147932/68‑1 (MQ=255) cGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:2228594/67‑1 (MQ=255) gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:3531149/51‑1 (MQ=255) gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa > 1:1669390/1‑68 (MQ=255) gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg < 1:5150326/71‑1 (MQ=255) gAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt < 1:2201812/47‑1 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc > 1:755256/1‑66 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc > 1:4962930/1‑66 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa > 1:413784/1‑71 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa > 1:1269982/1‑71 (MQ=255) tGCCGACAAAACCGGACGTGAGGAGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg > 1:4571971/1‑71 (MQ=255) | TCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGG > minE/1395623‑1395763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |