| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,395,708 | A→C | 14.3% | G138G (GGA→GGC) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,395,708 | 0 | A | C | 14.3% | 225.2 / 39.3 | 112 | G138G (GGA→GGC) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base A (44/52); new base C (9/7); total (53/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCG > minE/1395641‑1395777 | gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACAGGACGt < 1:5205782/71‑1 (MQ=255) cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATTCCGACAAAACCGGACGTg < 1:3663542/71‑1 (MQ=255) cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg < 1:1868552/71‑1 (MQ=255) tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc < 1:2859038/69‑1 (MQ=255) tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc < 1:4069707/69‑1 (MQ=255) ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg < 1:2464191/70‑1 (MQ=255) ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg < 1:655697/70‑1 (MQ=255) ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg < 1:3938887/70‑1 (MQ=255) gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg < 1:2601181/71‑1 (MQ=255) cTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACg < 1:1969996/60‑1 (MQ=255) tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt > 1:4926446/1‑71 (MQ=255) cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg > 1:3630451/1‑60 (MQ=255) cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg > 1:3794624/1‑71 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:5499068/71‑1 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:1754783/71‑1 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:1748280/71‑1 (MQ=255) aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt < 1:1246216/71‑1 (MQ=255) cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt > 1:5679529/1‑69 (MQ=255) cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt > 1:4275324/1‑69 (MQ=255) gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg > 1:2190858/1‑71 (MQ=255) cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc < 1:1596494/71‑1 (MQ=255) cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc > 1:449811/1‑71 (MQ=255) cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc > 1:3744981/1‑71 (MQ=255) aCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctct > 1:2786936/1‑71 (MQ=255) aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACg > 1:3234405/1‑56 (MQ=255) aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAgt < 1:3406017/68‑2 (MQ=255) ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt < 1:4381194/71‑1 (MQ=255) cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt < 1:5526964/71‑1 (MQ=255) cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt < 1:4016472/70‑1 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg < 1:4100766/55‑1 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt > 1:4897041/1‑71 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:2502730/71‑1 (MQ=255) gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt < 1:1207736/68‑1 (MQ=255) gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCAt > 1:1305554/1‑57 (MQ=255) cAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttttt < 1:3541979/71‑1 (MQ=255) aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt > 1:2876511/1‑70 (MQ=255) aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa > 1:2025824/1‑71 (MQ=255) aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAc > 1:170295/1‑71 (MQ=255) tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGa > 1:2205589/1‑38 (MQ=255) tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc > 1:828424/1‑39 (MQ=255) tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc > 1:567505/1‑39 (MQ=255) gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg > 1:4945487/1‑71 (MQ=255) gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg > 1:3163144/1‑71 (MQ=255) gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg > 1:4327109/1‑69 (MQ=255) tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTa < 1:661291/66‑1 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGt > 1:1917951/1‑37 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt > 1:686189/1‑62 (MQ=255) tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt < 1:2221757/59‑1 (MQ=255) tgcccttgcccGTGAACATGACGACAAAACCGGACGt > 1:1833024/1‑37 (MQ=255) gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt > 1:5071142/1‑69 (MQ=255) gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc < 1:3412457/70‑1 (MQ=255) gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc < 1:5692343/70‑1 (MQ=255) cccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCa < 1:1567975/70‑1 (MQ=255) cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt > 1:444304/1‑71 (MQ=255) cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt > 1:3063026/1‑71 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt < 1:4801565/40‑1 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt < 1:3018376/40‑1 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:2090912/57‑1 (MQ=255) ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAg < 1:1125024/68‑1 (MQ=255) tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt < 1:4686250/56‑1 (MQ=255) tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa > 1:839972/3‑59 (MQ=255) tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa > 1:3694005/3‑59 (MQ=255) cgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTAc > 1:3942509/2‑59 (MQ=255) gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:800569/70‑1 (MQ=255) cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa < 1:3983690/71‑1 (MQ=255) ccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa < 1:3915962/70‑1 (MQ=255) ccGAGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:2147932/68‑1 (MQ=255) cGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:2228594/67‑1 (MQ=255) gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa > 1:1669390/1‑68 (MQ=255) gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg < 1:5150326/71‑1 (MQ=255) gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg > 1:1913207/1‑71 (MQ=255) gAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt < 1:2201812/47‑1 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc > 1:755256/1‑66 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc > 1:4962930/1‑66 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa > 1:413784/1‑71 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa > 1:1269982/1‑71 (MQ=255) aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa < 1:3217458/71‑1 (MQ=255) tGCCGACAAAACCGGACGTGAGGAGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg > 1:4571971/1‑71 (MQ=255) ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:4097122/57‑1 (MQ=255) ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt < 1:4090419/57‑1 (MQ=255) ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc < 1:2122605/71‑1 (MQ=255) cGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt < 1:408682/49‑1 (MQ=255) gACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt < 1:3971058/71‑1 (MQ=255) aCAAAACTGGACGTGAGGCGGTGATGTTCAGCTCtttt < 1:1221231/38‑1 (MQ=255) aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt < 1:1438642/38‑1 (MQ=255) aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa > 1:3479667/1‑56 (MQ=255) aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa > 1:1879672/1‑56 (MQ=255) aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAtt < 1:5426095/71‑1 (MQ=255) cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt < 1:4366530/38‑1 (MQ=255) cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc > 1:2882481/1‑56 (MQ=255) aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCtttttt > 1:3911806/1‑38 (MQ=255) aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATg < 1:4625564/62‑1 (MQ=255) aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg > 1:160445/1‑71 (MQ=255) aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg > 1:4104137/1‑71 (MQ=255) aaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGcc > 1:4932554/1‑71 (MQ=255) aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg > 1:5235159/1‑38 (MQ=255) aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg > 1:1032836/1‑38 (MQ=255) cGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt > 1:2878861/1‑63 (MQ=255) gACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc < 1:1508296/59‑1 (MQ=255) gACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCg < 1:3737503/71‑1 (MQ=255) | GCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCG > minE/1395641‑1395777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |