| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,396,008 | C→T | 5.3% | N238N (AAC→AAT) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,396,008 | 0 | C | T | 5.3% | 287.9 / 3.4 | 94 | N238N (AAC→AAT) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base C (39/50); new base T (2/3); total (41/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
ACGCTGCTGCTGTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCC > minE/1395946‑1396077 | aCGCTGCTGCTGTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGc > 1:4964894/1‑70 (MQ=255) cGCTGCTGCTGTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCt < 1:5105210/70‑1 (MQ=255) gctgctgctGTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtat > 1:1000954/1‑71 (MQ=255) tgctgctgTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtatt > 1:569779/1‑70 (MQ=255) ctgctgTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACAt < 1:3880622/59‑1 (MQ=255) ctgctgTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtattt < 1:319693/69‑1 (MQ=255) ctgTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATg > 1:1060381/1‑57 (MQ=255) tttGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATc < 1:704981/69‑1 (MQ=255) tttGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATc < 1:653063/69‑1 (MQ=255) tttGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATc < 1:446060/69‑1 (MQ=255) tttGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATc < 1:552655/69‑1 (MQ=255) tGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCaa > 1:5250506/1‑69 (MQ=255) tGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAc > 1:2972197/1‑70 (MQ=255) tGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAAc > 1:3959260/1‑70 (MQ=255) gTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACg < 1:1843657/70‑1 (MQ=255) aTTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACt > 1:1882355/1‑71 (MQ=255) aTTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACt > 1:654201/1‑71 (MQ=255) tttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACg < 1:557893/66‑1 (MQ=255) tttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACg < 1:5656636/66‑1 (MQ=255) tttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTg < 1:851785/71‑1 (MQ=255) tttGTACATTGATGTGGGGCTCGAACAGCC‑‑TACATCATCAACATGCCGCTATTTATTATCAACg < 1:5669471/64‑1 (MQ=255) ttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATttatta < 1:4622908/59‑1 (MQ=255) ttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATttatta < 1:4495391/59‑1 (MQ=255) ttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATttatta < 1:1154904/59‑1 (MQ=255) ttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATttatta < 1:3509749/59‑1 (MQ=255) ttGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTg > 1:5586422/1‑70 (MQ=255) ctGTTCAATGATGTGGGCGGCGAATAATCTCTACATGATAAATATGCCGCTATTTATTATTGATGAACTg > 1:2536813/2‑70 (MQ=255) tGTTCAATGATGTGGGCGGCGAATAATCTCTACATGATAAATATGCCGCTATTtattatt < 1:955745/60‑2 (MQ=255) tGTTCAATGATGTGGGCGGCGAATAATCTCTACATGATAAATATGCCGCTATTTATTATTGATGAACTGCa > 1:4973423/1‑71 (MQ=255) tGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtatt > 1:5599928/1‑53 (MQ=255) tGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCa > 1:197016/1‑71 (MQ=255) gTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCaa > 1:3018465/1‑61 (MQ=255) aCATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtattta > 1:2934567/1‑52 (MQ=255) aCATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtattta > 1:1121831/1‑52 (MQ=255) cATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCtt < 1:3073421/71‑1 (MQ=255) aTTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAAc > 1:4877722/1‑62 (MQ=255) aTTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTc < 1:60312/71‑1 (MQ=255) aTTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTc < 1:1655155/71‑1 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtatttat > 1:4176034/1‑49 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtatttat > 1:5612835/1‑49 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCtatttat > 1:4668722/1‑49 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCtt > 1:2042681/1‑68 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTccc > 1:3670382/1‑71 (MQ=255) tGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTccc > 1:3514209/1‑71 (MQ=255) gATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGaa > 1:1339412/1‑58 (MQ=255) aTGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCga < 1:2398905/71‑1 (MQ=255) tgtgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgag < 1:3667849/71‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGc < 1:2412291/37‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGc < 1:3925178/37‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga < 1:2067995/70‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga < 1:3347261/70‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGaa < 1:3575806/71‑1 (MQ=255) tgGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGaa < 1:4257263/71‑1 (MQ=255) gCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTc < 1:1034926/60‑1 (MQ=255) gCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTc < 1:2114125/60‑1 (MQ=255) tCGAACAGCCTGTACATCATCAACATGCCGCtatt < 1:1338762/35‑1 (MQ=255) tCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGc > 1:4624053/1‑71 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATc < 1:810116/40‑1 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGa > 1:3130385/1‑45 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGa > 1:1340580/1‑45 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGa > 1:3867504/1‑45 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGa > 1:5034800/1‑45 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCg > 1:4434653/1‑71 (MQ=255) gAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCg < 1:4939742/71‑1 (MQ=255) aaCAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGa > 1:4155517/1‑44 (MQ=255) aCAGCCTGTACATCATCAACATGCCGCTATttatta > 1:400335/1‑36 (MQ=255) aCAGCCTGTACATCATCAACATGCCGCTATttatta > 1:5663360/1‑36 (MQ=255) aCAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt > 1:2819425/1‑71 (MQ=255) aCAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgt > 1:3574674/1‑71 (MQ=255) aGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCgg < 1:3203946/68‑1 (MQ=255) gCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCgg < 1:1967018/67‑1 (MQ=255) gCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGgtgt < 1:5342752/70‑1 (MQ=255) gCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGtgtg < 1:4254711/71‑1 (MQ=255) cTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat < 1:2847149/71‑1 (MQ=255) cTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgat < 1:5405958/71‑1 (MQ=255) tACATGATAAATATGCCGCTATTTATTATTGATGAACTGc < 1:4544882/40‑1 (MQ=12) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGc < 1:3200847/40‑1 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGc < 1:2061786/40‑1 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGtgatga < 1:172156/70‑1 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat > 1:2900126/1‑71 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat > 1:5058574/1‑71 (MQ=255) tACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat > 1:2076898/1‑71 (MQ=255) catgatAAATATGCCGCTATTTATTATTGATGAACTGCATCTAACCGATAAACTg < 1:507621/55‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGcc < 1:1072108/56‑1 (MQ=255) tcatcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGcc < 1:4310370/56‑1 (MQ=255) catcaACATGCCGCTATTTATTATCAACTAACTGCATCTTCCCGAGAAACTGGcc < 1:3175233/55‑1 (MQ=255) tcaACATGCCGCTATTTATTATCAACGAACTGCATCTTccc < 1:1708762/41‑1 (MQ=255) tcaACATGCCGCTATTTATTATCAACGAACTGCATCTTccc < 1:3708/41‑1 (MQ=255) tcaACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCgaga < 1:5406828/45‑1 (MQ=255) tcaACATGCCGCTATTTATTATCAAAGAACTGCATCTTCCCGAGAAACTGGCCGGTGTgatgat < 1:4699823/64‑1 (MQ=255) caACATGCCGCTATTTATTATCAACGAACTGCATCTTccc < 1:4090578/40‑1 (MQ=255) aaCATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccgc > 1:2834346/1‑71 (MQ=255) aCATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccgcc < 1:4852869/71‑1 (MQ=255) aCATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGAccgcc > 1:2268127/1‑71 (MQ=255) | ACGCTGCTGCTGTTTGTCATTTGTACATTGATGTGGGGCTCGAACAGCCTGTACATCATCAACATGCCGCTATTTATTATCAACGAACTGCATCTTCCCGAGAAACTGGCCGGTGTGATGATGGGGACCGCC > minE/1395946‑1396077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |