Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,398,448 A→C 6.4% I258L (ATC→CTC) ‡ yeiQ → predicted dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,398,4480AC 6.4% 250.0 / 9.2 94I258L (ATC→CTC) ‡yeiQpredicted dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (53/35);  new base C (4/2);  total (57/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGG  >  minE/1398385‑1398517
                                                               |                                                                     
cttgtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg                                                                <  1:1109242/68‑1 (MQ=255)
cTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg                                                                <  1:2667568/71‑1 (MQ=255)
cTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg                                                                <  1:3973047/71‑1 (MQ=255)
  ggtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                                              >  1:1165565/1‑71 (MQ=255)
   gtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggt                                                             >  1:3105828/1‑71 (MQ=255)
     ggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtgg                                                           >  1:4219094/1‑71 (MQ=255)
       aaaTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGgtggtg                                                         >  1:2352294/1‑71 (MQ=255)
        aaTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGtggtgg                                                        <  1:4597103/71‑1 (MQ=255)
         aTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtgg                                                           <  1:3399519/67‑1 (MQ=255)
            agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAg                                                     <  1:3657530/70‑1 (MQ=255)
            agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAg                                                     <  1:4715092/70‑1 (MQ=255)
                agcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAc                                                >  1:781736/1‑71 (MQ=255)
                   aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc                                             >  1:4034143/1‑71 (MQ=255)
                   aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc                                             >  1:4281641/1‑71 (MQ=255)
                      tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtg                                                            <  1:680593/53‑1 (MQ=255)
                      tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                                          >  1:3344106/1‑71 (MQ=255)
                      tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                                          >  1:3049965/1‑71 (MQ=255)
                       gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtcg                                         >  1:5083381/1‑71 (MQ=255)
                       gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                                          >  1:784404/1‑70 (MQ=255)
                       gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                                            <  1:2204438/68‑1 (MQ=255)
                        ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                                            <  1:4510589/67‑1 (MQ=255)
                        ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc                                        <  1:5491649/71‑1 (MQ=255)
                           gTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATc                                                                     <  1:210080/39‑1 (MQ=255)
                             taaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGaa                                                      <  1:2570899/51‑1 (MQ=255)
                             gAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGc                                   >  1:5566887/1‑71 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCa                                                                   >  1:4194354/1‑38 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg                                                                  >  1:3392752/1‑39 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg                                                                  >  1:1825352/1‑39 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg                                                                  >  1:1483074/1‑39 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg                                                                  >  1:4272767/1‑39 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg                                  >  1:4810909/1‑71 (MQ=255)
                              aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg                                  <  1:3486138/71‑1 (MQ=255)
                               atgatgCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt                                 >  1:4653900/1‑71 (MQ=255)
                               aTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg                                  >  1:4601743/1‑70 (MQ=255)
                               aTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt                                 >  1:2011444/1‑71 (MQ=255)
                                tGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg                                  <  1:5243313/69‑1 (MQ=255)
                                 gATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcc                               >  1:4286540/1‑71 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                                              >  1:4298412/1‑39 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                                              >  1:561118/1‑39 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                                              >  1:964430/1‑39 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa                                                       <  1:982987/46‑1 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa                                                       <  1:936633/46‑1 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa                                                       <  1:2904715/46‑1 (MQ=255)
                                  aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa                                                       <  1:1018479/46‑1 (MQ=255)
                                     ccTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgc                            >  1:5555090/1‑70 (MQ=255)
                                     ccTGCCCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc                           >  1:1063317/1‑71 (MQ=255)
                                      cTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGaa                                                      <  1:4585255/43‑1 (MQ=255)
                                       tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg                         >  1:4908960/1‑71 (MQ=255)
                                       tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg                         >  1:5059235/1‑71 (MQ=255)
                                       tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg                         >  1:3973929/1‑71 (MQ=255)
                                       tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt                                                   >  1:2744881/1‑45 (MQ=255)
                                       tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt                                                   >  1:178234/1‑45 (MQ=255)
                                       tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt                                                   >  1:2664134/1‑45 (MQ=255)
                                       tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt                                                   >  1:264899/1‑45 (MQ=255)
                                         cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGa                                                    >  1:2329784/1‑42 (MQ=255)
                                         cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTggg                       <  1:3198279/71‑1 (MQ=255)
                                         cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTgg                        <  1:1616006/70‑1 (MQ=255)
                                           cgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGa                      <  1:872647/70‑1 (MQ=255)
                                            gATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAg                    <  1:5332707/71‑1 (MQ=255)
                                             aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa                                                  >  1:902736/1‑40 (MQ=255)
                                             aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa                                                  >  1:4971611/1‑40 (MQ=255)
                                             aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa                                                  >  1:3543998/1‑40 (MQ=255)
                                             aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTgg                                     <  1:4211032/53‑1 (MQ=255)
                                             aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt                                 <  1:2727726/57‑1 (MQ=255)
                                               tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg                 >  1:3704498/1‑71 (MQ=255)
                                               tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg                 >  1:3223872/1‑71 (MQ=255)
                                               tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg                 >  1:4926710/1‑71 (MQ=255)
                                                aGCTTCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc                                        >  1:1006804/1‑47 (MQ=255)
                                                 gCTGCGAACCGTTTATCCAGTGTGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc                <  1:5582312/70‑1 (MQ=255)
                                                 gCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc                <  1:2425866/70‑1 (MQ=255)
                                                  cTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                                          <  1:2067472/43‑1 (MQ=255)
                                                   tGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTtcgtt                                          <  1:1344821/42‑2 (MQ=16)
                                                    gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgt                                           >  1:4686374/1‑40 (MQ=255)
                                                    gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCa               <  1:1394008/68‑1 (MQ=255)
                                                    gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgt            >  1:2661194/1‑71 (MQ=255)
                                                    gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgt            >  1:2264952/1‑71 (MQ=255)
                                                     cGAGCCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCa               >  1:2516092/1‑67 (MQ=255)
                                                     cGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTtcgtt                                          <  1:2285620/40‑2 (MQ=12)
                                                      gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctg                             >  1:1334294/1‑52 (MQ=255)
                                                      gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgtgt          <  1:4470716/71‑1 (MQ=255)
                                                       aaCCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                                          <  1:1095967/38‑1 (MQ=255)
                                                       aaCCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg                 <  1:1670057/63‑1 (MQ=255)
                                                       aaCCGTTTATCCAGTCGGTGGTGGAAGATAACTtcgtc                                          <  1:2734799/38‑1 (MQ=255)
                                                           gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa     >  1:4171660/1‑71 (MQ=255)
                                                           gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa     >  1:5299156/1‑71 (MQ=255)
                                                           gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa     >  1:5483649/1‑71 (MQ=255)
                                                           gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa     >  1:2523215/1‑71 (MQ=255)
                                                            tttATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg                                  <  1:4890295/41‑1 (MQ=255)
                                                            tttATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTCGGCGt                                 >  1:253146/1‑42 (MQ=255)
                                                              tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg                                      >  1:2253363/1‑35 (MQ=255)
                                                              tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg                                      >  1:4171726/1‑35 (MQ=255)
                                                              tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg                                      >  1:2199018/1‑35 (MQ=255)
                                                              tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTc                                >  1:4482699/1‑41 (MQ=255)
                                                              tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATgg  >  1:3425515/1‑71 (MQ=255)
                                                               |                                                                     
CTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGG  >  minE/1398385‑1398517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: