Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,398,555 G→C 6.8% R293R (CGG→CGC yeiQ → predicted dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,398,5550GC 6.8% 302.7 / 8.7 106R293R (CGG→CGCyeiQpredicted dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (34/58);  new base C (2/5);  total (39/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 113 total aligned reads displayed.

GTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATT  >  minE/1398485‑1398625
                                                                      |                                                                      
gTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGGGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCgg                                                                        <  1:4023698/71‑1 (MQ=255)
   ctgcctgGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATg                                                                     >  1:4482716/1‑71 (MQ=255)
      cctgGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCt                                                                   >  1:2505087/1‑70 (MQ=255)
          ggAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGa                                                                       >  1:2349932/1‑62 (MQ=255)
           gAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg                                                             <  1:3847408/71‑1 (MQ=255)
            aaGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTaa                                                                >  1:4062941/1‑67 (MQ=255)
             aGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                           <  1:1197201/71‑1 (MQ=255)
                 gCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCa                                                       <  1:4340410/71‑1 (MQ=255)
                     gtgtACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCActct                                                   >  1:4196684/1‑71 (MQ=255)
                      tgtACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCtt                                                  >  1:424754/1‑71 (MQ=255)
                        tACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg                                                             <  1:1343130/58‑1 (MQ=255)
                        tACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                >  1:1782652/1‑71 (MQ=255)
                         aCAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg                                                              >  1:4416057/1‑56 (MQ=255)
                         aCAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg                                                              >  1:186161/1‑56 (MQ=255)
                         aCAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg                                                              >  1:913662/1‑56 (MQ=255)
                          cAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTTCGGATGCTTAATGGCAGCCACTCTTTtc                                               <  1:3745041/70‑1 (MQ=255)
                          cAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTaa                                                                >  1:4112624/1‑53 (MQ=255)
                          cAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                              >  1:686893/1‑71 (MQ=255)
                          cAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtc                                               <  1:3461865/70‑1 (MQ=255)
                            aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                >  1:1077212/1‑67 (MQ=255)
                            aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                            >  1:5573882/1‑71 (MQ=255)
                            aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                            <  1:5186649/71‑1 (MQ=255)
                            aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                            >  1:1694403/1‑71 (MQ=255)
                             aTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAg                                                          >  1:4135741/1‑56 (MQ=255)
                             aTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGc                                           <  1:4365672/71‑1 (MQ=255)
                                gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                         <  1:5593535/70‑1 (MQ=255)
                                gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                         <  1:4777362/70‑1 (MQ=255)
                                gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                        <  1:5051443/71‑1 (MQ=255)
                                gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                        <  1:5078414/71‑1 (MQ=255)
                                gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                        >  1:4255604/1‑71 (MQ=255)
                                gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                        >  1:3027454/1‑71 (MQ=255)
                                   agcgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATc                                      <  1:2354516/67‑1 (MQ=255)
                                   aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                           <  1:1865461/49‑1 (MQ=255)
                                   aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                           <  1:1637501/49‑1 (MQ=255)
                                   aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                           <  1:1339574/49‑1 (MQ=255)
                                   aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCt                                     <  1:5027712/71‑1 (MQ=255)
                                    gcgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCt                                     <  1:3224720/68‑1 (MQ=255)
                                     cgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATc                                      <  1:1424359/67‑1 (MQ=255)
                                      gatgTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg                                   <  1:5159011/70‑1 (MQ=255)
                                        tgtgCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCa                                                       <  1:3985045/48‑1 (MQ=255)
                                        tgTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGtt                                <  1:4385565/71‑1 (MQ=255)
                                          tCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCttt                                                 <  1:3581651/52‑1 (MQ=255)
                                           ccTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAcc                             >  1:5546970/1‑71 (MQ=255)
                                            cTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                            <  1:547731/55‑1 (MQ=255)
                                               ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctct                          <  1:3320876/70‑1 (MQ=255)
                                               ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                           <  1:5654675/69‑1 (MQ=255)
                                               ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCCTTTCTCGCTTATCTGGGTTACctc                           <  1:243541/69‑1 (MQ=255)
                                                cATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGcc                                                        >  1:5021946/1‑39 (MQ=255)
                                                cATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAcc                             >  1:3515975/1‑66 (MQ=255)
                                                  tGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                              <  1:3519612/47‑1 (MQ=255)
                                                  tGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                              <  1:1962262/47‑1 (MQ=255)
                                                   gggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                    <  1:1739868/56‑1 (MQ=255)
                                                   gggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                    <  1:2749945/56‑1 (MQ=255)
                                                   atgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGa                     >  1:5199356/3‑71 (MQ=255)
                                                   atgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGa                     >  1:1029373/3‑71 (MQ=255)
                                                    ggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                    <  1:2370704/71‑1 (MQ=255)
                                                    ggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                    <  1:1049247/71‑1 (MQ=255)
                                                     gAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAtt                   >  1:489934/1‑71 (MQ=255)
                                                        gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                <  1:3620028/39‑1 (MQ=255)
                                                        gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                <  1:4618994/39‑1 (MQ=255)
                                                        gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                <  1:288878/39‑1 (MQ=255)
                                                        gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCtctc                         >  1:879195/1‑62 (MQ=255)
                                                        atgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTtct                                              <  1:4677885/39‑1 (MQ=19)
                                                         tgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTac                                       >  1:756699/2‑46 (MQ=16)
                                                         tgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTac                                       >  1:2217285/2‑46 (MQ=16)
                                                         agaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                <  1:1715180/38‑1 (MQ=255)
                                                         agaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc               <  1:2707655/71‑1 (MQ=255)
                                                         agaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc               <  1:5531092/71‑1 (MQ=255)
                                                          gaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTtct                                              <  1:2516500/39‑1 (MQ=21)
                                                          gaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc               <  1:3547853/70‑1 (MQ=255)
                                                           aTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTtctg                                             >  1:1802712/1‑38 (MQ=18)
                                                           aTGAAACTGCGTATGCTTAATGGCAGCCACTCTTTtc                                               <  1:2964225/37‑1 (MQ=255)
                                                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctct                          <  1:3071036/58‑1 (MQ=255)
                                                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                           <  1:2436884/57‑1 (MQ=255)
                                                           aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCa             >  1:4584046/1‑71 (MQ=255)
                                                            tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat            <  1:3566817/71‑1 (MQ=255)
                                                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                              <  1:2743233/36‑1 (MQ=255)
                                                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg                                  >  1:2800551/1‑48 (MQ=255)
                                                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg                                  >  1:3509646/1‑48 (MQ=255)
                                                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg                                   >  1:5109129/1‑47 (MQ=255)
                                                             gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat            <  1:2486661/70‑1 (MQ=255)
                                                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCt                                          >  1:103924/1‑39 (MQ=255)
                                                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTAt                                       >  1:4186710/1‑42 (MQ=255)
                                                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat          >  1:4617564/1‑71 (MQ=255)
                                                              aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat          >  1:4494058/1‑71 (MQ=255)
                                                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                    <  1:2001653/59‑1 (MQ=255)
                                                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                    <  1:729386/59‑1 (MQ=255)
                                                                aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTc                  <  1:2563694/61‑1 (MQ=255)
                                                                 ctgctgATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                         <  1:923099/37‑1 (MQ=255)
                                                                 cTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCtc                                    <  1:5095352/42‑2 (MQ=12)
                                                                 cTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAc                              <  1:669445/48‑1 (MQ=255)
                                                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                        <  1:838642/35‑1 (MQ=255)
                                                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTAt                                       >  1:3124372/1‑36 (MQ=255)
                                                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt      <  1:4640400/69‑1 (MQ=255)
                                                                    cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTg     <  1:3296696/70‑1 (MQ=255)
                                                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                    <  1:751561/38‑1 (MQ=255)
                                                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                    <  1:4890018/38‑1 (MQ=255)
                                                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                    <  1:5406986/38‑1 (MQ=255)
                                                                     ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGa    <  1:4052202/70‑1 (MQ=255)
                                                                      gATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGAtt  <  1:839277/71‑1 (MQ=255)
                                                                      |                                                                      
GTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATT  >  minE/1398485‑1398625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: