Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,398,564 T→C 18.5% N296N (AAT→AAC yeiQ → predicted dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,398,5640TC18.5% 266.4 / 47.0 119N296N (AAT→AACyeiQpredicted dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (31/66);  new base C (8/14);  total (39/80)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 121 total aligned reads displayed.

GAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAG  >  minE/1398496‑1398633
                                                                    |                                                                     
gAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg                                                                     <  1:3847408/71‑1 (MQ=255)
     cGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGc                                                                 <  1:5517684/70‑1 (MQ=255)
          gtgtACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCActct                                                           >  1:4196684/1‑71 (MQ=255)
           tgtACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCtt                                                          >  1:424754/1‑71 (MQ=255)
             tACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg                                                                     <  1:1343130/58‑1 (MQ=255)
             tACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                        >  1:1782652/1‑71 (MQ=255)
              aCAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg                                                                      >  1:913662/1‑56 (MQ=255)
              aCAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg                                                                      >  1:4416057/1‑56 (MQ=255)
               cAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTTCGGATGCTTAATGGCAGCCACTCTTTtc                                                       <  1:3745041/70‑1 (MQ=255)
                aaaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtctc                                                     <  1:4067791/71‑1 (MQ=255)
                 aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                        >  1:1077212/1‑67 (MQ=255)
                 aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                                    >  1:1694403/1‑71 (MQ=255)
                 aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                                    <  1:5186649/71‑1 (MQ=255)
                 aaTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                                    >  1:5573882/1‑71 (MQ=255)
                  aTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAg                                                                  >  1:4135741/1‑56 (MQ=255)
                  aTGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGc                                                   <  1:4365672/71‑1 (MQ=255)
                     gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                 <  1:5593535/70‑1 (MQ=255)
                     gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                >  1:3027454/1‑71 (MQ=255)
                     gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                <  1:5078414/71‑1 (MQ=255)
                     gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                >  1:4255604/1‑71 (MQ=255)
                     gTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                <  1:5051443/71‑1 (MQ=255)
                        agcgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATc                                              <  1:2354516/67‑1 (MQ=255)
                        aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                                   <  1:1865461/49‑1 (MQ=255)
                        aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                                   <  1:1339574/49‑1 (MQ=255)
                        aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCa                                                                   <  1:2456918/49‑1 (MQ=255)
                        aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCTGCCAct                                                             <  1:736249/55‑1 (MQ=255)
                        aaTGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCt                                             <  1:5027712/71‑1 (MQ=255)
                         gcgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCt                                             <  1:3224720/68‑1 (MQ=255)
                          cgatgTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATc                                              <  1:1424359/67‑1 (MQ=255)
                           gatgTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCActc                                                            <  1:3597602/53‑1 (MQ=255)
                             tgtgCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCa                                                               <  1:3985045/48‑1 (MQ=255)
                             tgTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGtt                                        <  1:4385565/71‑1 (MQ=255)
                               tCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                 <  1:4665547/60‑1 (MQ=255)
                                ccTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAcc                                     >  1:5546970/1‑71 (MQ=255)
                                 cTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCg                                                    <  1:547731/55‑1 (MQ=255)
                                    ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctct                                  <  1:3320876/70‑1 (MQ=255)
                                    ccATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                                   <  1:5654675/69‑1 (MQ=255)
                                     cATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGcc                                                                >  1:5021946/1‑39 (MQ=255)
                                     cATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAcc                                     >  1:3515975/1‑66 (MQ=255)
                                       tGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                                      <  1:3519612/47‑1 (MQ=255)
                                       tGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                                      <  1:1962262/47‑1 (MQ=255)
                                        gggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                            <  1:1739868/56‑1 (MQ=255)
                                        gggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                            <  1:2749945/56‑1 (MQ=255)
                                        atgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGa                             >  1:5199356/3‑71 (MQ=255)
                                        atgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGa                             >  1:1029373/3‑71 (MQ=255)
                                         tgAAGAGATGAAGTTGCGCATGCTCAACGGCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCAGGAt                            <  1:1907401/70‑1 (MQ=255)
                                         ggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                            <  1:1049247/71‑1 (MQ=255)
                                         ggAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                            <  1:2370704/71‑1 (MQ=255)
                                          gAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAtt                           >  1:489934/1‑71 (MQ=255)
                                             gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                        <  1:3620028/39‑1 (MQ=255)
                                             gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                        <  1:4618994/39‑1 (MQ=255)
                                             gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                        <  1:288878/39‑1 (MQ=255)
                                             gagaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCtctc                                 >  1:879195/1‑62 (MQ=255)
                                             atgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTtct                                                      <  1:4677885/39‑1 (MQ=19)
                                              tgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTac                                               >  1:2217285/2‑46 (MQ=16)
                                              tgaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTac                                               >  1:756699/2‑46 (MQ=16)
                                              agaTGAAACTGCGGATGCTTAATGGCAGCCACTCtttt                                                        <  1:1715180/38‑1 (MQ=255)
                                               gaTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTtct                                                      <  1:2516500/39‑1 (MQ=21)
                                               gaTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc                       <  1:3547853/70‑1 (MQ=255)
                                                aTGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTtctg                                                     >  1:1802712/1‑38 (MQ=18)
                                                aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctct                                  <  1:3071036/58‑1 (MQ=255)
                                                aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                                   <  1:1556137/57‑1 (MQ=255)
                                                aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACctc                                   <  1:2436884/57‑1 (MQ=255)
                                                 tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat                    <  1:3566817/71‑1 (MQ=255)
                                                 tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat                    <  1:3302335/71‑1 (MQ=255)
                                                  gAAACTGCGGATGCTTAATGGCAGCCACTCTTTtct                                                      <  1:2743233/36‑1 (MQ=255)
                                                  gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg                                          >  1:3509646/1‑48 (MQ=255)
                                                  gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTggg                                          >  1:2800551/1‑48 (MQ=255)
                                                  gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTgg                                           >  1:5109129/1‑47 (MQ=255)
                                                  gAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat                    <  1:2486661/70‑1 (MQ=255)
                                                   aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCt                                                  >  1:103924/1‑39 (MQ=255)
                                                   aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat                  >  1:4494058/1‑71 (MQ=255)
                                                   aaaCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat                  >  1:4617564/1‑71 (MQ=255)
                                                     aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                            <  1:2001653/59‑1 (MQ=255)
                                                     aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGAt                            <  1:729386/59‑1 (MQ=255)
                                                     aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTc                          <  1:2563694/61‑1 (MQ=255)
                                                     aCTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCatat                  >  1:278798/1‑69 (MQ=255)
                                                      ctgctgATGCTTAATGGCAGCCACTCTTTTCTCGCtt                                                 <  1:923099/37‑1 (MQ=255)
                                                      cTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCtc                                            <  1:5095352/42‑2 (MQ=12)
                                                      cTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTAc                                      <  1:669445/48‑1 (MQ=255)
                                                         cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTa                                                <  1:838642/35‑1 (MQ=255)
                                                         cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTAt                                               >  1:3124372/1‑36 (MQ=255)
                                                         cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt              <  1:4640400/69‑1 (MQ=255)
                                                         cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTg             <  1:3296696/70‑1 (MQ=255)
                                                          ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                            <  1:4890018/38‑1 (MQ=255)
                                                          ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                            <  1:751561/38‑1 (MQ=255)
                                                          ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTg                                            <  1:5406986/38‑1 (MQ=255)
                                                          ggATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGa            <  1:4052202/70‑1 (MQ=255)
                                                           taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                     <  1:3023955/43‑1 (MQ=16)
                                                           taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                     <  1:43793/43‑1 (MQ=16)
                                                           taTGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTAcc                                     <  1:2209461/43‑1 (MQ=16)
                                                            aTGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg         <  1:4027587/71‑1 (MQ=255)
                                                             tGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACctc                                   >  1:4265212/1‑44 (MQ=16)
                                                             tGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACctc                                   >  1:3064531/1‑44 (MQ=16)
                                                                ttAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTAGCCCATATCAGTGATtgcatg     >  1:1332273/1‑71 (MQ=255)
                                                                 tAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCa                     >  1:884494/1‑54 (MQ=255)
                                                                  aaTGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatg     <  1:1182832/69‑1 (MQ=255)
                                                                   aTGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAg  >  1:5126400/1‑71 (MQ=255)
                                                                    tGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc    <  1:3728052/68‑1 (MQ=255)
                                                                    tGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc    <  1:1939867/68‑1 (MQ=255)
                                                                    |                                                                     
GAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAG  >  minE/1398496‑1398633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: