| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 106,064 | T→G | 11.4% | A76A (GCA→GCC) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 106,064 | 0 | T | G | 11.4% | 75.6 / 5.5 | 35 | A76A (GCA→GCC) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (13/18); new base G (2/2); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01 | |||||||||||
TTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGG > minE/105999‑106134 | ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc < 1:361639/71‑1 (MQ=255)ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc < 1:3509339/71‑1 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:994333/1‑71 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:1694569/1‑71 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:4309736/1‑71 (MQ=255) cagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttct > 1:1899357/1‑71 (MQ=255) gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac > 1:2519690/1‑71 (MQ=255) gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac > 1:4109909/1‑71 (MQ=255) aaaaCTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGaccac > 1:5197546/1‑63 (MQ=255) cTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAg < 1:5270303/71‑1 (MQ=255) tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt < 1:5349701/70‑1 (MQ=255) tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt < 1:1085254/70‑1 (MQ=255) gCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt > 1:2700314/1‑71 (MQ=255) aGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCg > 1:1605763/1‑70 (MQ=255) aGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc < 1:606703/71‑1 (MQ=255) gTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCa > 1:805067/1‑58 (MQ=255) tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttcttc < 1:2509002/44‑1 (MQ=255) aTTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCGCAt > 1:1419409/1‑70 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg < 1:856727/63‑1 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATg < 1:5494395/70‑1 (MQ=255) aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt > 1:3789774/1‑53 (MQ=255) aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAgg > 1:4151564/1‑71 (MQ=255) gTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTTACCCa < 1:2182475/47‑1 (MQ=255) tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAtt < 1:3799355/39‑1 (MQ=255) tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAtt < 1:4094996/39‑1 (MQ=255) tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt < 1:178533/71‑1 (MQ=255) tGGCTAAAGGAGCATGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt < 1:482131/71‑1 (MQ=255) aaGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc > 1:3476152/1‑70 (MQ=255) aGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCa < 1:2706303/71‑1 (MQ=255) gAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:3640472/71‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa > 1:3768620/1‑71 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:168769/71‑1 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:815917/71‑1 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:2768478/71‑1 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:452202/71‑1 (MQ=255) tGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:2659560/66‑1 (MQ=255) tGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATgg > 1:924761/1‑71 (MQ=255) | TTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGG > minE/105999‑106134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |