Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,439,278 G→A 10.1% L342L (CTG→TTG)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,439,2780GA10.1% 475.3 / 28.7 168L342L (CTG→TTG) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (89/62);  new base A (7/10);  total (96/72)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

AAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACA  >  minE/1439209‑1439348
                                                                     |                                                                      
 aaCCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                      <  1:4138392/71‑1 (MQ=255)
 aaCCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagc                                                                       <  1:3289041/70‑1 (MQ=255)
   ccAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                                                                    <  1:4212303/71‑1 (MQ=255)
          cAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                                             <  1:266062/71‑1 (MQ=255)
           agagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                      >  1:1495636/1‑61 (MQ=255)
           agagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                      >  1:1502076/1‑61 (MQ=255)
           agagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                                                            >  1:861140/1‑71 (MQ=255)
           agagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                                                            >  1:4740888/1‑71 (MQ=255)
            gagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTTGTCCAGCAGGTTGATTTTg                                                           <  1:736171/71‑1 (MQ=255)
            gagCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                                                           >  1:2556152/1‑71 (MQ=255)
             agCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                                                        <  1:2226363/57‑1 (MQ=255)
             agCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                                                        <  1:5072974/57‑1 (MQ=255)
             agCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                                                        <  1:4170026/57‑1 (MQ=255)
               cTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                                                        >  1:4458924/1‑71 (MQ=255)
                  cGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                                      <  1:5637433/70‑1 (MQ=255)
                       ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                  >  1:3940912/1‑69 (MQ=255)
                         aTCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                      >  1:375317/1‑47 (MQ=255)
                             gtgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg                                           >  1:2460578/1‑70 (MQ=255)
                              tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                              <  1:3775057/66‑1 (MQ=255)
                              tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                              <  1:3381070/66‑1 (MQ=255)
                              tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                              <  1:5355698/66‑1 (MQ=255)
                              tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                              <  1:1046173/66‑1 (MQ=255)
                                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                                                           >  1:1212090/1‑51 (MQ=255)
                                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                         >  1:1028837/1‑53 (MQ=255)
                                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                         >  1:4586367/1‑53 (MQ=255)
                                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                         >  1:1183250/1‑53 (MQ=255)
                                ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg                                            >  1:5321850/1‑66 (MQ=255)
                                  gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                     >  1:2038727/1‑71 (MQ=255)
                                  gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGATTGATTTTGTAGTCAACGTAGGTGGACATGt                                     >  1:824490/1‑71 (MQ=255)
                                   aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                                    >  1:4381702/1‑71 (MQ=255)
                                   aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                                    >  1:2530451/1‑71 (MQ=255)
                                   aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                                    <  1:778097/71‑1 (MQ=255)
                                   aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                                    <  1:2947650/71‑1 (MQ=255)
                                   aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                     <  1:5205859/70‑1 (MQ=255)
                                    tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                   >  1:3884185/1‑71 (MQ=255)
                                     gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                  >  1:3396512/1‑71 (MQ=255)
                                     gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                  >  1:1636361/1‑71 (MQ=255)
                                      ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                  <  1:5478143/70‑1 (MQ=255)
                                      ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                  <  1:5367519/70‑1 (MQ=255)
                                       cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                <  1:2709638/71‑1 (MQ=255)
                                       cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                <  1:2314737/71‑1 (MQ=255)
                                       cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                >  1:3092386/1‑71 (MQ=255)
                                          cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                             <  1:3392749/71‑1 (MQ=255)
                                          cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                              >  1:1091368/1‑70 (MQ=255)
                                          cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGCACAt                                       >  1:2442057/1‑61 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                                                             >  1:5597814/1‑35 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCaa                                                    >  1:2770774/1‑44 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                              >  1:4316277/1‑50 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGAc                                         >  1:267152/1‑55 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                           >  1:4328330/1‑69 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                          <  1:3443245/70‑1 (MQ=255)
                                              aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                          >  1:4193704/1‑70 (MQ=255)
                                                gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag                       >  1:503619/1‑71 (MQ=255)
                                                  gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                 <  1:595353/43‑1 (MQ=255)
                                                  gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag                       >  1:208755/1‑69 (MQ=255)
                                                  gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                     <  1:3003898/71‑1 (MQ=255)
                                                   tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag                    <  1:4993626/71‑1 (MQ=255)
                                                   tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag                    >  1:3675444/1‑71 (MQ=255)
                                                     aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                               >  1:1019975/1‑42 (MQ=255)
                                                     aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                  <  1:3413120/71‑1 (MQ=255)
                                                     aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                   >  1:2870853/1‑70 (MQ=255)
                                                     aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                   >  1:225726/1‑70 (MQ=255)
                                                     aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                   >  1:3529130/1‑70 (MQ=255)
                                                      aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                   >  1:2175317/1‑69 (MQ=255)
                                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACa                                        >  1:2309420/1‑47 (MQ=255)
                                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                       >  1:4341750/1‑48 (MQ=255)
                                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                       >  1:48516/1‑48 (MQ=255)
                                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                       >  1:4651639/1‑48 (MQ=255)
                                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                <  1:108110/71‑1 (MQ=255)
                                                       cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                <  1:4665887/71‑1 (MQ=255)
                                                         ggTTGTCGTCCAGCAGTTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                             >  1:1873684/1‑56 (MQ=255)
                                                         ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa              >  1:3062769/1‑71 (MQ=255)
                                                         ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa              >  1:5450907/1‑71 (MQ=255)
                                                         ggTTGTCGTCCAGCAGGTTGATTTTGTAGCCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa              >  1:1678382/1‑71 (MQ=255)
                                                           ttGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                            <  1:4080371/55‑1 (MQ=255)
                                                             gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa          >  1:2783014/4‑71 (MQ=18)
                                                             gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa          >  1:2572929/1‑71 (MQ=255)
                                                             gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa          >  1:5410912/1‑71 (MQ=255)
                                                             gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca           <  1:266456/70‑1 (MQ=255)
                                                               cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca        >  1:3199365/1‑71 (MQ=255)
                                                               catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAaata                        >  1:1942366/3‑55 (MQ=18)
                                                               catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCca           <  1:2721209/66‑1 (MQ=17)
                                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                 >  1:3977898/1‑45 (MQ=255)
                                                                gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat       >  1:4390068/1‑71 (MQ=255)
                                                                gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatat       >  1:4026394/1‑71 (MQ=18)
                                                                atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt                                  <  1:1146107/43‑1 (MQ=21)
                                                                atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt                                  <  1:2838655/43‑1 (MQ=21)
                                                                atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata        <  1:4728259/69‑3 (MQ=17)
                                                                atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata        <  1:335678/69‑3 (MQ=17)
                                                                 tcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                 <  1:4061150/44‑1 (MQ=255)
                                                                 tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttttat                               >  1:2509889/1‑44 (MQ=21)
                                                                 tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc      <  1:1550976/71‑1 (MQ=17)
                                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                >  1:5512792/1‑59 (MQ=255)
                                                                   cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa    >  1:2143839/1‑71 (MQ=255)
                                                                   cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaa    >  1:1837273/1‑71 (MQ=18)
                                                                   cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca     <  1:4983761/70‑1 (MQ=17)
                                                                     gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca  <  1:3425900/71‑1 (MQ=255)
                                                                     gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca  >  1:3324712/1‑71 (MQ=255)
                                                                     gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca  >  1:1599128/1‑71 (MQ=255)
                                                                     gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg  <  1:5693118/71‑2 (MQ=17)
                                                                     |                                                                      
AAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACA  >  minE/1439209‑1439348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: