| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,300 | G→A | 16.6% | Y334Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,300 | 0 | G | A | 16.6% | 561.3 / 83.9 | 234 | Y334Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (104/91); new base A (21/18); total (125/109) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 242 total aligned reads displayed.
TTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGT > minE/1439232‑1439370 | ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg > 1:3940912/1‑69 (MQ=255)ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg > 1:771291/1‑69 (MQ=255)ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:4031249/71‑1 (MQ=255) tCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg > 1:38354/1‑70 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:1046173/66‑1 (MQ=255) tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg < 1:5355698/66‑1 (MQ=255) ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:5274048/1‑71 (MQ=255) gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt > 1:271094/1‑71 (MQ=255) gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt > 1:1085518/1‑71 (MQ=255) gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGATTGATTTTGTAGTCAACGTAGGTGGACATGt > 1:824490/1‑71 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt < 1:2947650/71‑1 (MQ=255) aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt < 1:4147216/70‑1 (MQ=255) tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt > 1:3884185/1‑71 (MQ=255) gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt > 1:1636361/1‑71 (MQ=255) ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:5478143/70‑1 (MQ=255) cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg < 1:2314737/71‑1 (MQ=255) cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg > 1:3092386/1‑71 (MQ=255) aGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt < 1:3381229/68‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:2661346/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:2061745/1‑70 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGCACAt > 1:2442057/1‑61 (MQ=255) gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:4625872/71‑1 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag > 1:4193704/1‑70 (MQ=255) gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag > 1:503619/1‑71 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:407646/43‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt < 1:2667403/43‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa < 1:2777514/44‑1 (MQ=255) gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta < 1:3003898/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg > 1:1019975/1‑42 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:3655631/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:3413120/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg < 1:2133898/70‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:2870853/1‑70 (MQ=255) aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:2175317/1‑69 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:48516/1‑48 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:4341750/1‑48 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt > 1:4003433/1‑48 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:4665887/71‑1 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:5450907/1‑71 (MQ=255) gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt > 1:3393273/1‑62 (MQ=255) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa < 1:3520931/68‑1 (MQ=18) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2572929/1‑71 (MQ=255) tcatcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa > 1:2288634/4‑70 (MQ=17) catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAaata > 1:1942366/3‑55 (MQ=18) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:1502786/1‑47 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:4390068/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:2913728/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:5407005/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCa > 1:3334123/1‑64 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt < 1:1146107/43‑1 (MQ=21) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:335678/69‑3 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:2830113/69‑3 (MQ=17) tcCAGCAGGTTGATTCTGTAGTCAACGTAGGTGGACATGttttt < 1:2523792/44‑1 (MQ=255) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttttat > 1:1058379/1‑44 (MQ=21) cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaa > 1:1837273/1‑71 (MQ=18) cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca < 1:4983761/70‑1 (MQ=17) caacagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGcc < 1:5209956/60‑1 (MQ=14) agcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:5516660/45‑1 (MQ=17) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:1599128/1‑71 (MQ=255) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:3425900/71‑1 (MQ=255) gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:5693118/71‑2 (MQ=17) cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat > 1:2701219/1‑65 (MQ=17) cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat > 1:1673454/1‑65 (MQ=17) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTttg > 1:3980827/1‑39 (MQ=14) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTttg > 1:1902300/1‑39 (MQ=14) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:2416003/41‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:2497682/47‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag < 1:3031492/43‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:1990063/51‑1 (MQ=255) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc < 1:4986682/57‑1 (MQ=14) ttGATTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:4690003/39‑1 (MQ=14) ttGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAttt < 1:4368525/71‑1 (MQ=9) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:1251756/1‑57 (MQ=255) tGATTTTGTAATCAACATAGGTGGATATGTTTTTGTTGAAGTAGTAGGTCGCACcaa > 1:4956813/1‑57 (MQ=14) tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAag < 1:3504904/41‑1 (MQ=14) gATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt < 1:1391926/36‑1 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3949071/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:5196568/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2546674/1‑69 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:894945/71‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:4293262/71‑1 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:2376032/36‑1 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt > 1:316245/1‑47 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt > 1:2030702/1‑47 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:1517902/1‑68 (MQ=17) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:1787158/1‑68 (MQ=17) ttttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag > 1:2612646/1‑38 (MQ=255) tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc < 1:377474/48‑1 (MQ=14) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata > 1:4428765/1‑71 (MQ=255) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:4159667/1‑61 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:3442550/1‑64 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:3201786/1‑64 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:4759256/1‑64 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac < 1:4089155/53‑1 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac < 1:617216/53‑1 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:5060699/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:4268644/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:3721277/1‑70 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:701866/1‑71 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt < 1:1198628/71‑1 (MQ=255) | TTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGT > minE/1439232‑1439370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |