| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,414 | C→G | 16.2% | L296L (CTG→CTC) ‡ | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,414 | 0 | C | G | 16.2% | 332.7 / 45.3 | 130 | L296L (CTG→CTC) ‡ | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (46/62); new base G (9/12); total (56/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
AACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCC > minE/1439345‑1439483 | aacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc < 1:5253107/70‑1 (MQ=255) acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:2265582/1‑71 (MQ=255) acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:2539654/1‑71 (MQ=255) acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:222512/1‑71 (MQ=255) atatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg > 1:192306/1‑70 (MQ=255) atatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg > 1:1765487/1‑70 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg < 1:1095544/71‑1 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg > 1:668731/1‑71 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg > 1:3325632/1‑71 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg < 1:4508043/71‑1 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac < 1:4979120/70‑1 (MQ=255) ttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg > 1:3540881/1‑70 (MQ=255) tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg < 1:3578828/65‑1 (MQ=255) tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg < 1:1319975/65‑1 (MQ=255) aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:747491/71‑1 (MQ=255) gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:1458373/71‑1 (MQ=255) tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:4449484/69‑1 (MQ=255) tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGa < 1:2167186/71‑1 (MQ=255) atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc > 1:4839122/1‑71 (MQ=255) ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg > 1:2203836/1‑70 (MQ=255) tcgtcgtcgTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga > 1:964194/1‑60 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:4221174/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:1738365/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:2602749/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:4553075/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:2506492/70‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa < 1:4591558/69‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg > 1:1134832/1‑71 (MQ=255) gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt > 1:3647463/1‑71 (MQ=255) tcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc < 1:1669508/48‑1 (MQ=255) cgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc > 1:2020717/1‑71 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc < 1:1064020/70‑1 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt > 1:4348782/1‑71 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc < 1:3198147/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:5365246/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:5136905/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:4766235/70‑1 (MQ=255) ccACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg < 1:4403093/42‑1 (MQ=255) aCCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:1614130/1‑36 (MQ=255) ccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg < 1:4215898/39‑1 (MQ=255) aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga > 1:3386334/1‑39 (MQ=255) aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc < 1:5095140/71‑1 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:5534720/1‑55 (MQ=255) tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:2070705/47‑1 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:2234658/37‑1 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:104976/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:3046131/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt > 1:5083192/1‑71 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGCGACGGACGCAGACCGAAGTCg > 1:282336/1‑50 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:957795/36‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:5096822/1‑51 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc < 1:5545305/63‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:1710827/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:359277/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:3856771/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:1406890/70‑1 (MQ=255) cttACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACt > 1:3008237/2‑53 (MQ=12) ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt > 1:2741014/1‑46 (MQ=255) tACCTTTAGATTGCAGGTAAGAGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt > 1:2668514/1‑57 (MQ=255) tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa > 1:5181823/1‑63 (MQ=255) tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc < 1:4635071/70‑1 (MQ=255) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:1537705/70‑2 (MQ=18) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:2125179/70‑2 (MQ=18) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:229860/70‑1 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:492745/70‑1 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3167355/1‑70 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:2820980/70‑1 (MQ=255) ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:2128302/69‑2 (MQ=18) cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg > 1:1664479/1‑71 (MQ=255) cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg > 1:4586901/1‑71 (MQ=255) ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt > 1:2825957/1‑71 (MQ=16) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGATGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:2920638/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:1420880/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt > 1:640514/1‑70 (MQ=255) tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt < 1:1950475/69‑1 (MQ=12) tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt < 1:4315465/70‑1 (MQ=255) aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa > 1:2729144/1‑48 (MQ=2) aGATTGCAGGTAAGCAACGGACGGACGCAGACCGAAATCGAACTGATATTGTGCAACAGCTTCGAAGtttt > 1:4744142/1‑69 (MQ=17) aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc < 1:3576373/70‑1 (MQ=255) gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt < 1:2782426/70‑1 (MQ=255) cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg < 1:839806/71‑1 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca > 1:1757761/1‑37 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt < 1:3774929/71‑1 (MQ=255) gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc < 1:1866340/54‑1 (MQ=9) gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:5621379/1‑71 (MQ=12) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt < 1:305950/35‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt < 1:5455682/35‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt < 1:1653707/35‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:5203468/63‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:4573596/63‑1 (MQ=255) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:5201831/71‑1 (MQ=12) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:212433/71‑1 (MQ=12) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:1279895/71‑1 (MQ=12) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:4205124/71‑1 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa > 1:741568/1‑47 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc > 1:3181866/1‑49 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:3673739/1‑71 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTccca < 1:4463286/71‑4 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:3970357/1‑70 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:4290362/1‑70 (MQ=255) | AACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCC > minE/1439345‑1439483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |