| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,780 | G→A | 9.6% | R1031R (CGC→CGT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,780 | 0 | G | A | 9.6% | 144.0 / 5.1 | 52 | R1031R (CGC→CGT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (20/27); new base A (2/3); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCC > minE/309710‑309848 | aTTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg > 1:213003/1‑71 (MQ=255) tACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg > 1:4857399/1‑71 (MQ=255) tACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg > 1:1765905/1‑71 (MQ=255) acacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg > 1:5509266/1‑71 (MQ=255) acacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg > 1:5186827/1‑71 (MQ=255) acacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg > 1:4655138/1‑70 (MQ=255) gTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa > 1:3241305/1‑71 (MQ=255) gtttATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac > 1:1055826/4‑71 (MQ=255) tCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc < 1:1343530/59‑1 (MQ=255) tCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg > 1:918399/1‑58 (MQ=255) tCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg > 1:722164/1‑58 (MQ=255) tCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg > 1:4774689/1‑58 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:938070/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:372150/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:3270433/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:3263763/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:1622046/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCCGCGGCGa > 1:3959701/1‑62 (MQ=255) aaTGATGATCGACAGTATGGCTGTGATCGATATCTTCATTCTTGCGGCTAAAgcggcgg > 1:1550917/1‑59 (MQ=255) tgatgaTCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg < 1:3217467/59‑1 (MQ=255) tgatgaTCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca > 1:5110062/1‑69 (MQ=255) atgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg > 1:2303031/1‑70 (MQ=255) tCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATAc > 1:2835169/1‑71 (MQ=255) cGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc < 1:4057310/48‑1 (MQ=255) cGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa < 1:4874872/54‑1 (MQ=255) aGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGa < 1:3452607/49‑1 (MQ=255) aGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGa < 1:3586751/49‑1 (MQ=255) gCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa > 1:4735029/1‑69 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa < 1:417756/50‑1 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa < 1:2417350/50‑1 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa < 1:3756945/50‑1 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3083854/51‑1 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3002248/50‑1 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt > 1:3632136/1‑71 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACAACAAAGAATACCGGAACgaag > 1:5068710/1‑65 (MQ=255) cTCGATATCTTCATTCTTGCGGCTATAGCGGCGGCGAACCACCACAAAGAATAGCGGAACgtagaa < 1:2436516/66‑1 (MQ=38) tCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:5370036/1‑40 (MQ=255) tCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:4044873/1‑40 (MQ=255) tCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:5692974/1‑40 (MQ=255) atatCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3299348/45‑1 (MQ=255) tCTTCATTCTTGCGGCTAAAGCGGCGGCGAACTACCACAAAGAATACCGGAAc < 1:3705274/53‑1 (MQ=255) ttCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg > 1:3118460/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:3062001/1‑71 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt > 1:726645/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:5632875/70‑1 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:5639346/70‑1 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGa > 1:1629771/1‑35 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAt > 1:2279661/1‑54 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt < 1:1037312/69‑1 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt < 1:4802089/69‑1 (MQ=255) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:221571/2‑71 (MQ=16) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:1446701/2‑71 (MQ=16) cTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:5569396/58‑1 (MQ=255) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:3829365/57‑1 (MQ=255) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg < 1:1550459/60‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:2154671/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:1254904/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:701302/70‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa < 1:611669/42‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc > 1:3255651/1‑71 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACg > 1:3643413/1‑36 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:2545637/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3879147/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3234260/69‑1 (MQ=255) aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:3422099/1‑71 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATT‑CCAGTACCGTTGCGGTCACCATcc > 1:4777737/1‑66 (MQ=255) ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:3265306/70‑1 (MQ=255) | ATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCC > minE/309710‑309848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |