| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,432 | G→A | 17.6% | F290F (TTC→TTT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,432 | 0 | G | A | 17.6% | 310.0 / 45.9 | 125 | F290F (TTC→TTT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (45/58); new base A (9/13); total (54/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 133 total aligned reads displayed.
TTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGC > minE/1439363‑1439502 | ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGCCCg > 1:513285/1‑70 (MQ=255)ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg > 1:2203836/1‑70 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:2602749/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:4553075/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:4221174/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:1738365/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa < 1:4591558/69‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg > 1:1134832/1‑71 (MQ=255) gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTGGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:2625254/69‑1 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc < 1:1064020/70‑1 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc < 1:4714787/70‑1 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt > 1:4348782/1‑71 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc < 1:3198147/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:4766235/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:2769725/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:5365246/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:5136905/70‑1 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:5534720/1‑55 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:3569540/1‑55 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa < 1:3138662/71‑1 (MQ=255) tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:2070705/47‑1 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:104976/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:152100/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt > 1:5083192/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:5096822/1‑51 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc < 1:5545305/63‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:359277/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:1710827/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:3856771/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:1406890/70‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:2948643/70‑1 (MQ=255) cttACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACt > 1:3008237/2‑53 (MQ=12) ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt > 1:2741014/1‑46 (MQ=255) tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa > 1:5181823/1‑63 (MQ=255) tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc < 1:4635071/70‑1 (MQ=255) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACtt > 1:3224646/1‑68 (MQ=18) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:1537705/70‑2 (MQ=18) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc > 1:207291/1‑69 (MQ=18) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:2820980/70‑1 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:492745/70‑1 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3167355/1‑70 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:229860/70‑1 (MQ=255) ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:2128302/69‑2 (MQ=18) cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg > 1:1664479/1‑71 (MQ=255) ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt > 1:2825957/1‑71 (MQ=16) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:1420880/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt > 1:640514/1‑70 (MQ=255) tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt < 1:4497202/70‑1 (MQ=16) tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt < 1:3856330/70‑1 (MQ=16) tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt < 1:1950475/69‑1 (MQ=12) aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa > 1:2729144/1‑48 (MQ=2) aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc < 1:3576373/70‑1 (MQ=255) cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg < 1:839806/71‑1 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca > 1:1757761/1‑37 (MQ=255) ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt < 1:1927208/68‑1 (MQ=255) gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc < 1:1866340/54‑1 (MQ=9) gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:5621379/1‑71 (MQ=12) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt < 1:1653707/35‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt < 1:305950/35‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:4577851/63‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:4573596/63‑1 (MQ=255) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:5201831/71‑1 (MQ=12) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:212433/71‑1 (MQ=12) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:1279895/71‑1 (MQ=12) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:5024250/62‑1 (MQ=255) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:4205124/71‑1 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa > 1:741568/1‑47 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:2821390/1‑71 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:2164181/1‑71 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:5202142/1‑71 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:3673739/1‑71 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTccca < 1:4463286/71‑4 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:4290362/1‑70 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:3970357/1‑70 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:3738549/71‑1 (MQ=255) tggacgggcGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:473578/66‑1 (MQ=12) gggACGGACGCAGACCGAAGTCTAACTGGTACTGAGCAAc < 1:279155/40‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc < 1:5607030/40‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc < 1:1054902/40‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc < 1:4756293/40‑1 (MQ=255) ggacggacGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt < 1:525522/51‑1 (MQ=255) gacggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAcc < 1:888507/71‑1 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:5427483/67‑1 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:477419/71‑1 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:1827755/71‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc < 1:5247982/64‑1 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:5569083/50‑1 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg < 1:5348626/71‑1 (MQ=255) gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:2896100/1‑71 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg < 1:4631618/71‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg < 1:4712528/71‑1 (MQ=255) cgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg > 1:2599974/1‑47 (MQ=255) gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:3715535/1‑48 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:2363513/1‑55 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc > 1:3203689/1‑54 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt > 1:1518147/1‑70 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa > 1:2885762/1‑71 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg > 1:2961451/1‑71 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg > 1:242296/1‑71 (MQ=255) gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc < 1:5295041/71‑1 (MQ=255) | TTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGC > minE/1439363‑1439502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |