| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,468 | C→T | 12.2% | Q278Q (CAG→CAA) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,468 | 0 | C | T | 12.2% | 294.1 / 22.5 | 106 | Q278Q (CAG→CAA) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (44/49); new base T (6/7); total (50/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
AGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCC > minE/1439399‑1439537 | aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc < 1:3576373/70‑1 (MQ=255) gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt < 1:2782426/70‑1 (MQ=255) cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg < 1:839806/71‑1 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt < 1:3774929/71‑1 (MQ=255) ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt < 1:1927208/68‑1 (MQ=255) ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:5580003/71‑1 (MQ=255) gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt > 1:5621379/1‑71 (MQ=12) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:978039/63‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:4577851/63‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:4573596/63‑1 (MQ=255) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:1279895/71‑1 (MQ=12) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:5201831/71‑1 (MQ=12) tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:212433/71‑1 (MQ=12) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:5024250/62‑1 (MQ=255) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:4205124/71‑1 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:2164181/1‑71 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:2821390/1‑71 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:5202142/1‑71 (MQ=255) aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg > 1:3673739/1‑71 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTccca < 1:4463286/71‑4 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:3970357/1‑70 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:3738549/71‑1 (MQ=255) tggacgggcGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:473578/66‑1 (MQ=12) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt < 1:1573870/54‑1 (MQ=255) gacggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAcc < 1:888507/71‑1 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:4511539/67‑1 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc < 1:477419/71‑1 (MQ=255) cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc < 1:5247982/64‑1 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg < 1:5569083/50‑1 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg < 1:5348626/71‑1 (MQ=255) gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:2896100/1‑71 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg < 1:4631618/71‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg < 1:4695601/71‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg < 1:4712528/71‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg < 1:515938/71‑1 (MQ=255) acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:1440325/1‑70 (MQ=255) cgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg > 1:2599974/1‑47 (MQ=255) gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa > 1:3235858/1‑71 (MQ=255) gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt > 1:3715535/1‑48 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc > 1:2363513/1‑55 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc > 1:3203689/1‑54 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt > 1:1518147/1‑70 (MQ=255) aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa > 1:2885762/1‑71 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg > 1:2961451/1‑71 (MQ=255) cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg > 1:242296/1‑71 (MQ=255) gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc < 1:1203482/71‑1 (MQ=255) gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc < 1:5295041/71‑1 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg < 1:2871320/71‑1 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc > 1:309436/1‑70 (MQ=255) aaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt > 1:1954970/1‑47 (MQ=19) gTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg < 1:548413/71‑1 (MQ=255) tCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt < 1:4932016/43‑1 (MQ=16) cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGtt > 1:4982332/1‑71 (MQ=255) cGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcga > 1:5212954/1‑46 (MQ=16) gAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGtt > 1:5099683/1‑70 (MQ=255) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGttag > 1:4403302/1‑42 (MQ=12) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGttag > 1:263830/1‑42 (MQ=12) gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGttag > 1:5417320/1‑42 (MQ=12) aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg > 1:964801/1‑71 (MQ=255) aCTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:2531925/43‑1 (MQ=12) aCTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:5258728/43‑1 (MQ=12) cTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg > 1:799047/1‑51 (MQ=255) cTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGa > 1:667362/1‑64 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:260644/1‑36 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt < 1:4844501/71‑1 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg > 1:5360290/1‑71 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg < 1:2328281/70‑1 (MQ=255) gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGa > 1:2398863/1‑56 (MQ=255) gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTc > 1:5426898/1‑71 (MQ=255) gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGACTTGCGTTGTAGGTc > 1:240772/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGt < 1:606913/69‑1 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg < 1:5049327/52‑1 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg < 1:3520154/52‑1 (MQ=255) cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa < 1:2003798/45‑1 (MQ=255) cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg > 1:4918357/1‑71 (MQ=255) cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg > 1:2475661/1‑71 (MQ=255) tgCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg > 1:5155091/2‑36 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCa > 1:340815/1‑35 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCa > 1:3086118/1‑35 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtgt < 1:402799/71‑1 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg < 1:4885115/70‑1 (MQ=255) gCTTCGAATTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa > 1:1782947/1‑71 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa > 1:2002851/1‑71 (MQ=255) cTTCGAAGTTCTGTGCTTTGTTCTCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTAc < 1:1516407/71‑1 (MQ=255) cTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg < 1:407557/53‑1 (MQ=255) tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:3710470/1‑70 (MQ=255) cGAAGTTCTGTGCTTTGTTCGCCCAACCCATGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTAc > 1:3775049/1‑68 (MQ=255) gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt > 1:5518644/1‑63 (MQ=255) gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt < 1:2191437/68‑1 (MQ=255) gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag > 1:4792552/1‑71 (MQ=255) gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag > 1:3892365/1‑71 (MQ=255) aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt > 1:907154/1‑66 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:3555233/48‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt < 1:2082820/49‑1 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTa > 1:611593/1‑48 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc > 1:1679602/1‑69 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc > 1:3553910/1‑69 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc > 1:717388/1‑69 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc > 1:2253485/1‑69 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGcc < 1:2287588/70‑1 (MQ=255) | AGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCC > minE/1439399‑1439537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |