| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,798 | A→C | 20.5% | V168V (GTT→GTG) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,798 | 0 | A | C | 20.5% | 244.5 / 71.8 | 127 | V168V (GTT→GTG) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (44/57); new base C (16/10); total (60/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAC > minE/1439731‑1439868 | gACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa > 1:5640637/1‑69 (MQ=255) gttgttAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa < 1:2955158/70‑1 (MQ=255) ttgttAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa > 1:2907751/1‑69 (MQ=255) gttagttaCGCCACTATTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg < 1:4691544/70‑1 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg < 1:867747/70‑1 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGcc > 1:2776364/1‑71 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:901969/1‑71 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:4028265/1‑71 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACa > 1:5563721/1‑50 (MQ=255) tAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg < 1:946638/53‑1 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:2616335/1‑61 (MQ=255) ttCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag > 1:3829570/1‑70 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa > 1:3144134/1‑48 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:2568228/1‑57 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:2246951/1‑57 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:4472787/1‑57 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:4820904/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:4628469/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:4258669/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:1775763/1‑71 (MQ=255) aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt < 1:4362760/71‑1 (MQ=255) aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt < 1:5050667/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt > 1:4219703/1‑70 (MQ=255) aGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa < 1:3519884/37‑1 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:1593116/1‑53 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag < 1:4076419/63‑1 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag < 1:2264004/63‑1 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:346052/1‑71 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:1500305/1‑71 (MQ=255) tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:594962/71‑1 (MQ=255) ggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa < 1:2939076/71‑1 (MQ=255) ttGCCGTTTTTACCCTTGTACTGAACAGCAAAGtt < 1:3747349/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:2788405/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:3148972/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:194325/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:5095300/63‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:2691678/63‑1 (MQ=255) ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAgt < 1:1222257/61‑1 (MQ=2) ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga > 1:245171/3‑69 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:2365396/70‑1 (MQ=255) ccGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:3448231/53‑1 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:3481802/1‑71 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:4051572/1‑71 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:4609455/1‑40 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:3235629/1‑70 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt < 1:5418776/71‑1 (MQ=255) ttgttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGt < 1:4163262/68‑1 (MQ=9) ttattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt > 1:944137/4‑71 (MQ=255) ttattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt > 1:5490431/4‑71 (MQ=255) tattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGtt > 1:2472725/3‑70 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa > 1:2124127/1‑41 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa > 1:5206816/1‑41 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:2293748/1‑71 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:5234665/1‑71 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcg > 1:2122834/2‑71 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc < 1:1903393/69‑1 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc < 1:4359271/69‑1 (MQ=255) ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc < 1:3486189/71‑1 (MQ=9) ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:4326007/47‑1 (MQ=255) ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:755752/47‑1 (MQ=255) tACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc > 1:1925733/1‑70 (MQ=9) tACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc > 1:1432098/1‑70 (MQ=9) aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:77650/1‑70 (MQ=255) aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:2255134/1‑70 (MQ=255) aCCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa > 1:4607544/1‑69 (MQ=9) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGCAGAAGTCAGTGTTACGGTAGGTCGCGaa < 1:4488359/71‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGtt < 1:1886572/56‑1 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa < 1:446008/70‑3 (MQ=255) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc > 1:4504168/4‑70 (MQ=9) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc > 1:1718842/4‑70 (MQ=9) ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:2497059/1‑53 (MQ=255) ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg > 1:1261773/1‑71 (MQ=255) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAGTCAGTATTACGATAAGTCGCGACGcca > 1:3367110/3‑70 (MQ=9) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGcca > 1:5310415/3‑70 (MQ=9) gTACTGAACAGCAAAGTTCAGGCCTTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:2621710/71‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc < 1:888923/69‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc < 1:1149380/69‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg < 1:4788670/70‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:798438/71‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:1097808/1‑71 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:4839570/71‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:2312503/1‑71 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:3983863/1‑71 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCGGTGTTACGGTAGGTCGCGAAGc < 1:5030844/67‑1 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:5506495/71‑1 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:2037482/71‑1 (MQ=255) aCTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa > 1:5538641/1‑64 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:4804220/52‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:2206403/52‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:5518907/35‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:5617523/35‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:1269071/41‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:3644829/41‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAg < 1:1015214/43‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:1523523/68‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag < 1:3798414/35‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:4521908/41‑1 (MQ=255) aaCAGCAAAGTTCAGGCCGTGAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa > 1:4705293/1‑60 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa > 1:1589268/1‑60 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:4472473/1‑71 (MQ=255) | GACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAC > minE/1439731‑1439868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |