| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,801 | A→C | 15.8% | A167A (GCT→GCG) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,801 | 0 | A | C | 15.8% | 297.5 / 51.2 | 133 | A167A (GCT→GCG) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (52/60); new base C (12/9); total (64/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
GTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGC > minE/1439735‑1439870 | gttgttAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa < 1:2955158/70‑1 (MQ=255) ttgttAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa > 1:2907751/1‑69 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg < 1:867747/70‑1 (MQ=255) aCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGcc > 1:2776364/1‑71 (MQ=255) gCCACTAGTAAAGCCTTCACAAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGt < 1:2417803/71‑1 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:4028265/1‑71 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACa > 1:5563721/1‑50 (MQ=255) aaGCGTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc > 1:4789897/1‑58 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:2616335/1‑61 (MQ=255) ttCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag > 1:3829570/1‑70 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa > 1:3144134/1‑48 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:2246951/1‑57 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:2544921/1‑57 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:1775763/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:4628469/1‑71 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag > 1:4820904/1‑71 (MQ=255) aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt < 1:447477/71‑1 (MQ=255) aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt < 1:5050667/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt > 1:4219703/1‑70 (MQ=255) aGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa < 1:3519884/37‑1 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:1593116/1‑53 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:829005/1‑71 (MQ=255) gATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:346052/1‑71 (MQ=255) aTGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc < 1:3463405/49‑1 (MQ=255) tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:594962/71‑1 (MQ=255) ggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa < 1:2939076/71‑1 (MQ=255) ttGCCGTTTTTACCCTTGTACTGAACAGCAAAGtt < 1:3747349/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:194325/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:4288928/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:3148972/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt < 1:2788405/35‑1 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:5095300/63‑1 (MQ=255) ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAgt < 1:1222257/61‑1 (MQ=2) ttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACga > 1:245171/3‑69 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg > 1:2116530/1‑36 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:2365396/70‑1 (MQ=255) tcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACgat > 1:5514461/2‑68 (MQ=2) cGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACgat > 1:2673864/1‑67 (MQ=9) gttgttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATaa < 1:913249/70‑2 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:4051572/1‑71 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg > 1:3481802/1‑71 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg < 1:1208302/48‑1 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt < 1:5418776/71‑1 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt > 1:3365252/1‑71 (MQ=255) ttgttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGt < 1:4163262/68‑1 (MQ=9) ttattACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGt > 1:944137/4‑71 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa > 1:5206816/1‑41 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa > 1:2124127/1‑41 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:5234665/1‑71 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:2293748/1‑71 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcg > 1:1540319/2‑71 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcg > 1:2122834/2‑71 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc < 1:1903393/69‑1 (MQ=255) gttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTc < 1:4359271/69‑1 (MQ=255) ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc < 1:399180/71‑1 (MQ=9) ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc < 1:5324948/71‑1 (MQ=9) ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:755752/47‑1 (MQ=255) ttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:4326007/47‑1 (MQ=255) tACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc > 1:1925733/1‑70 (MQ=9) tACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc > 1:1432098/1‑70 (MQ=9) aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:2255134/1‑70 (MQ=255) aCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgcg > 1:77650/1‑70 (MQ=255) aCCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa > 1:4607544/1‑69 (MQ=9) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGCAGAAGTCAGTGTTACGGTAGGTCGCGaa < 1:4488359/71‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGtt < 1:1886572/56‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgc < 1:2288497/68‑1 (MQ=255) cccTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgcaa < 1:446008/70‑3 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:1428588/71‑1 (MQ=255) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGc > 1:1718842/4‑70 (MQ=9) ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:2497059/1‑53 (MQ=255) ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg > 1:1261773/1‑71 (MQ=255) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAGTCAGTATTACGATAAGTCGCGACGcca > 1:3367110/3‑70 (MQ=9) gTACTGAACAGCAAAGTTCAGGCCTTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:2621710/71‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc < 1:888923/69‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc < 1:4409602/69‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg < 1:4788670/70‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:4839570/71‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt > 1:1097808/1‑71 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCGGTGTTACGGTAGGTCGCGAAGc < 1:5030844/67‑1 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:5506495/71‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:5617523/35‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:5518907/35‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:2209405/41‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:3644829/41‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAg < 1:1015214/43‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc < 1:4635692/64‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt < 1:1523523/68‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag < 1:3798414/35‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa < 1:4521908/41‑1 (MQ=255) aaCAGCAAAGTTCAGGCCGTGAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa > 1:4705293/1‑60 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa > 1:4595357/1‑60 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:4472473/1‑71 (MQ=255) aCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:316611/1‑41 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc < 1:5579924/71‑1 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc < 1:1538613/71‑1 (MQ=255) aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg > 1:5216332/2‑51 (MQ=255) aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg > 1:72759/2‑51 (MQ=255) aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg > 1:3733531/2‑51 (MQ=255) aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg > 1:3497126/2‑51 (MQ=255) aaGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAgg > 1:1010531/2‑51 (MQ=255) | GTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGC > minE/1439735‑1439870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |