| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,788 | C→T | 11.5% | V1029I (GTT→ATT) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,788 | 0 | C | T | 11.5% | 137.2 / 7.3 | 52 | V1029I (GTT→ATT) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (16/30); new base T (3/3); total (19/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
TTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACC > minE/309718‑309857 | gtttATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac > 1:1055826/4‑71 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:372150/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:1622046/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:3270433/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:3263763/71‑1 (MQ=255) aaTGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca < 1:938070/71‑1 (MQ=255) tgatgaTCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca > 1:5110062/1‑69 (MQ=255) atgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg > 1:2303031/1‑70 (MQ=255) tCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATAc > 1:2835169/1‑71 (MQ=255) gCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa > 1:4735029/1‑69 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa < 1:3756945/50‑1 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa < 1:417756/50‑1 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaaa < 1:2417350/50‑1 (MQ=255) tgCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3083854/51‑1 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3002248/50‑1 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt > 1:3632136/1‑71 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACAACAAAGAATACCGGAACgaag > 1:5068710/1‑65 (MQ=255) cTCGATATCTTCATTCTTGCGGCTATAGCGGCGGCGAACCACCACAAAGAATAGCGGAACgtagaa < 1:2436516/66‑1 (MQ=38) tCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:5370036/1‑40 (MQ=255) tCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:4044873/1‑40 (MQ=255) tCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca > 1:5692974/1‑40 (MQ=255) atatCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAg < 1:3299348/45‑1 (MQ=255) tCTTCATTCTTGCGGCTAAAGCGGCGGCGAACTACCACAAAGAATACCGGAAc < 1:3705274/53‑1 (MQ=255) ttCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg > 1:3118460/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:3062001/1‑71 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt > 1:726645/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:5632875/70‑1 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:5639346/70‑1 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGa > 1:1629771/1‑35 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAt > 1:2279661/1‑54 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt < 1:1037312/69‑1 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt < 1:4802089/69‑1 (MQ=255) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:1446701/2‑71 (MQ=16) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:221571/2‑71 (MQ=16) cTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:5569396/58‑1 (MQ=255) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:3829365/57‑1 (MQ=255) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg < 1:1550459/60‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:701302/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:2154671/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:1254904/70‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa < 1:611669/42‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc > 1:3255651/1‑71 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACg > 1:3643413/1‑36 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3879147/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3234260/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:2545637/69‑1 (MQ=255) aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:3422099/1‑71 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATT‑CCAGTACCGTTGCGGTCACCATcc > 1:4777737/1‑66 (MQ=255) ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:3265306/70‑1 (MQ=255) aacggcggATCACCACAAAGAACACCGGTACGAAGAAGATTg > 1:956319/3‑42 (MQ=18) cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc < 1:4354259/53‑1 (MQ=255) cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc < 1:3891948/53‑1 (MQ=255) cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc < 1:2851986/53‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTAGCg < 1:1792654/47‑1 (MQ=255) cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:4164197/70‑1 (MQ=255) aaCCACCACAAAGAATA‑CGGAACGAAGAAGATTGCCAGTAc > 1:3598405/1‑41 (MQ=38) accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc > 1:4442604/1‑71 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAg < 1:2936979/37‑1 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa > 1:5409611/1‑63 (MQ=255) | TTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACC > minE/309718‑309857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |