| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,801 | T→C | 20.0% | V1024V (GTA→GTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,801 | 0 | T | C | 20.0% | 124.7 / 24.2 | 60 | V1024V (GTA→GTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (20/28); new base C (7/5); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01 | |||||||||||
TCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTG > minE/309733‑309868 | tCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATAc > 1:2835169/1‑71 (MQ=255) gCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa > 1:4735029/1‑69 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt > 1:3632136/1‑71 (MQ=255) gCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACAACAAAGAATACCGGAACgaag > 1:5068710/1‑65 (MQ=255) cTCGATATCTTCATTCTTGCGGCTATAGCGGCGGCGAACCACCACAAAGAATAGCGGAACgtagaa < 1:2436516/66‑1 (MQ=38) tCTTCATTCTTGCGGCTAAAGCGGCGGCGAACTACCACAAAGAATACCGGAAc < 1:3705274/53‑1 (MQ=255) ttCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg > 1:3118460/1‑71 (MQ=255) tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt > 1:3062001/1‑71 (MQ=255) cATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt > 1:726645/1‑71 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:5639346/70‑1 (MQ=255) aTTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt < 1:5632875/70‑1 (MQ=255) cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAt > 1:2279661/1‑54 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt < 1:1037312/69‑1 (MQ=255) gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt < 1:4802089/69‑1 (MQ=255) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:221571/2‑71 (MQ=16) ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:1446701/2‑71 (MQ=16) cTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:5569396/58‑1 (MQ=255) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCg < 1:3829365/57‑1 (MQ=255) tAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg < 1:1550459/60‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:701302/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:2154671/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:1254904/70‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa < 1:611669/42‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc > 1:3255651/1‑71 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACg > 1:3643413/1‑36 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:2545637/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3879147/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3234260/69‑1 (MQ=255) aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:3422099/1‑71 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATT‑CCAGTACCGTTGCGGTCACCATcc > 1:4777737/1‑66 (MQ=255) ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:3265306/70‑1 (MQ=255) aacggcggATCACCACAAAGAACACCGGTACGAAGAAGATTg > 1:956319/3‑42 (MQ=18) cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc < 1:4354259/53‑1 (MQ=255) cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc < 1:2851986/53‑1 (MQ=255) cggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc < 1:3891948/53‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTAGCg < 1:1792654/47‑1 (MQ=255) cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:4164197/70‑1 (MQ=255) aaCCACCACAAAGAATA‑CGGAACGAAGAAGATTGCCAGTAc > 1:3598405/1‑41 (MQ=38) accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc > 1:4442604/1‑71 (MQ=255) tcaccacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTAcccc > 1:314986/2‑70 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAg < 1:2936979/37‑1 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa > 1:5409611/1‑63 (MQ=255) accacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:3281504/59‑1 (MQ=255) accacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTaccc > 1:5354213/1‑65 (MQ=16) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTAc > 1:532980/1‑36 (MQ=255) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:1440765/57‑1 (MQ=255) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa > 1:4718558/1‑59 (MQ=255) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc > 1:4126667/1‑71 (MQ=255) acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc > 1:5554540/1‑71 (MQ=255) caAAGAATACCGGAACTAAGAAGATTGCCAGTACCGTTGCGGTCAc < 1:1065477/46‑1 (MQ=255) caAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTTCGGTCAc < 1:4336929/46‑1 (MQ=255) caAAGAATACCGGAACCAAGAAGATTGCCAGGACCGTTGCGGCCAc < 1:5163425/46‑1 (MQ=255) aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:5162098/1‑70 (MQ=255) aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca > 1:849491/1‑68 (MQ=12) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:625993/67‑1 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:847248/67‑1 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:182003/67‑1 (MQ=255) aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc < 1:31127/52‑1 (MQ=12) aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc < 1:973145/52‑1 (MQ=12) gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag > 1:2923753/1‑69 (MQ=255) tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:5670355/1‑65 (MQ=255) tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:2475731/1‑65 (MQ=255) | TCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTG > minE/309733‑309868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |