| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,951 | A→G | 14.1% | V117V (GTT→GTC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,951 | 0 | A | G | 14.1% | 259.4 / 26.8 | 99 | V117V (GTT→GTC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (45/40); new base G (6/8); total (51/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
GTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGG > minE/1439882‑1440020 | gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca > 1:4046471/1‑70 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACg < 1:2857174/70‑1 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACg > 1:534128/1‑70 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5161832/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:77581/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5414727/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5192443/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5586634/1‑71 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:3815406/71‑1 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:4179201/71‑1 (MQ=255) gTAGGTGTCGCCACCGAATTCTGGCAGAACGTCGGTCCAGGAAGTTACGTCGTAAACAACGCCGTAGTTAc > 1:5286585/1‑71 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:2183552/64‑1 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:5600500/70‑1 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg < 1:1385996/71‑1 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg > 1:485216/1‑71 (MQ=255) gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGtt > 1:2382272/1‑63 (MQ=255) gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg > 1:1314500/1‑70 (MQ=255) gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt > 1:1029332/2‑71 (MQ=2) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg < 1:234209/70‑1 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTc > 1:3122079/1‑70 (MQ=255) caccAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTc > 1:3933879/1‑70 (MQ=2) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGa > 1:2560936/1‑61 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc > 1:5221540/1‑63 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc > 1:5682205/1‑63 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:3548694/71‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:1899483/71‑1 (MQ=255) accGAATTATGGCAGTACGTCGGTACAGGAAGTTACGTCATAaacaa > 1:3301902/1‑47 (MQ=255) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCg > 1:2077768/1‑70 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3865498/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3858876/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:2183233/71‑1 (MQ=2) caaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa > 1:2805084/3‑69 (MQ=2) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:2565344/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:4681827/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:3116911/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:4875436/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:1224023/1‑68 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:4759651/71‑1 (MQ=255) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:1639409/67‑1 (MQ=9) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAg > 1:4231383/2‑71 (MQ=2) aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca > 1:5266472/1‑42 (MQ=255) ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:259777/53‑1 (MQ=255) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:2916126/60‑1 (MQ=2) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:1568840/60‑1 (MQ=2) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTa > 1:357278/1‑46 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:2263981/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:1653329/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCCTa < 1:4663147/58‑1 (MQ=255) cTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGaaa > 1:3960016/1‑65 (MQ=2) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:5207718/42‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:3922148/45‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATcc < 1:556987/71‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:5182644/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:379425/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:748351/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:4545436/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:379437/57‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAACCAACGCCGTAGTTa > 1:2268116/1‑42 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:3183425/40‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:5438572/1‑42 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:3098874/1‑61 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:1853459/1‑61 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg < 1:4442729/71‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:4490468/70‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:1887784/70‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1407141/55‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1064637/55‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:5367911/68‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3516121/70‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3756184/70‑1 (MQ=255) gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATc < 1:3583432/62‑1 (MQ=255) gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:803002/1‑71 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg > 1:4526654/1‑49 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:1061861/1‑68 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:2205639/1‑68 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa > 1:1133195/1‑69 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:5313212/1‑71 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:2011459/1‑71 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt > 1:3406862/1‑38 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt > 1:1089521/1‑38 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGaa > 1:4401152/1‑60 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGc > 1:78193/1‑70 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg < 1:1933110/71‑1 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg < 1:584078/71‑1 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg < 1:1157656/71‑1 (MQ=255) ctACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:5472907/38‑1 (MQ=17) ctACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:107261/38‑1 (MQ=17) tACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCa > 1:3218599/1‑62 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:2195251/40‑1 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGaa > 1:1402119/1‑42 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGaa > 1:806747/1‑42 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGa < 1:5567178/70‑1 (MQ=255) tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGc > 1:5628671/1‑62 (MQ=255) tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:5311221/1‑70 (MQ=255) aaaCAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca > 1:773917/1‑71 (MQ=255) aacaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAt > 1:4470062/1‑64 (MQ=255) acaacGACGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa > 1:735530/1‑54 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg > 1:1844581/1‑71 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg < 1:2057451/71‑1 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg < 1:3226849/71‑1 (MQ=255) | GTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGG > minE/1439882‑1440020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |