| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,440,185 | G→A | 7.6% | D39D (GAC→GAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,440,185 | 0 | G | A | 7.6% | 283.4 / 8.2 | 92 | D39D (GAC→GAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (45/40); new base A (5/2); total (50/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCG > minE/1440118‑1440251 | tGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTc > 1:225469/1‑70 (MQ=255) aaGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa < 1:4625807/70‑1 (MQ=255) aaGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa < 1:4991474/70‑1 (MQ=255) gCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACtt < 1:3949881/71‑1 (MQ=255) ccAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACttt < 1:1641231/71‑1 (MQ=255) aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTa > 1:3048947/1‑70 (MQ=255) aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:5072382/1‑71 (MQ=255) aGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt > 1:3094189/1‑61 (MQ=255) aGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTc < 1:1982002/62‑1 (MQ=255) aGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTc < 1:3971222/62‑1 (MQ=255) aGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTc < 1:5561920/62‑1 (MQ=255) gACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg > 1:4437036/1‑71 (MQ=255) aCGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg > 1:4789128/1‑70 (MQ=255) cGCATGTATGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa < 1:4228482/71‑1 (MQ=255) cATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACa < 1:5384074/71‑1 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTc < 1:4241986/55‑1 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa > 1:4168794/1‑57 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa > 1:2733721/1‑57 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa > 1:834612/1‑57 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg < 1:4823278/65‑1 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa < 1:1611298/71‑1 (MQ=255) tAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc > 1:371044/1‑71 (MQ=255) tAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc > 1:757451/1‑71 (MQ=255) ggTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:243293/1‑59 (MQ=255) ggTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:595585/1‑59 (MQ=255) tCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt < 1:106870/71‑1 (MQ=255) cTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGTCCGTCTACTTTACCGTACAGATCTAAtt > 1:46530/1‑71 (MQ=255) gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCGGTCTACTTTACCGTACAGATCTAAttt < 1:2837429/69‑1 (MQ=255) gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAttt < 1:5245665/69‑1 (MQ=255) gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt < 1:4054538/71‑1 (MQ=255) tCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgtt < 1:3972393/71‑1 (MQ=255) tCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgtt < 1:4415298/71‑1 (MQ=255) ccATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGcc > 1:3906280/1‑71 (MQ=255) ccATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGcc < 1:624218/71‑1 (MQ=255) cATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACtt > 1:4776643/1‑45 (MQ=255) cATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCg > 1:689984/1‑71 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAg < 1:5521815/53‑1 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAg < 1:5394859/53‑1 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAg < 1:5167924/53‑1 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCAGTCTACTTTACCGTACAGATCTAAtt < 1:3704217/61‑1 (MQ=255) cTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCt > 1:3663035/1‑71 (MQ=255) cTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCt > 1:2346539/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACtt > 1:4413362/1‑41 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa > 1:4845125/1‑48 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa > 1:78174/1‑48 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:3347306/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:1934871/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:2625383/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:2906427/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:4393193/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:431957/1‑71 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCTTCTttg < 1:180053/71‑1 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCt < 1:2752969/68‑1 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttg < 1:3816773/71‑1 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttg < 1:696502/71‑1 (MQ=255) aTCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg < 1:4857748/43‑1 (MQ=255) aTCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg < 1:914361/43‑1 (MQ=255) tCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt < 1:780484/54‑1 (MQ=255) tCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt > 1:4338540/1‑58 (MQ=255) cTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:5208711/1‑39 (MQ=255) ttgttgTCAGAGAAATAGTGCAGGCCGTCTACttt < 1:5200954/35‑1 (MQ=255) ttgttgTCAGAGAAATAGTGCAGGCCGTCTACttt < 1:1651955/35‑1 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa < 1:4519547/70‑1 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:2978238/1‑70 (MQ=255) gttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa < 1:3901941/70‑1 (MQ=255) ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATaa > 1:4584766/4‑69 (MQ=2) ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:5075339/4‑71 (MQ=9) tgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt > 1:5530487/1‑64 (MQ=255) atCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATttgt > 1:1072543/2‑50 (MQ=2) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt < 1:2968300/70‑1 (MQ=255) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt > 1:323894/1‑70 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACa > 1:3062996/1‑35 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACa > 1:3978740/1‑35 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt < 1:4981965/43‑1 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt > 1:63663/1‑47 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt > 1:1637962/1‑47 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:2106970/1‑71 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:4428153/1‑71 (MQ=255) aaaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAAttt < 1:3358368/41‑1 (MQ=2) gaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagcag > 1:1661661/1‑71 (MQ=255) aaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGt > 1:5335882/2‑50 (MQ=14) aaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcggctg > 1:1430497/2‑69 (MQ=9) aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:3737092/1‑65 (MQ=255) aGTGCAGGCCGTCTACTTTTCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt > 1:1521748/1‑71 (MQ=255) aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca > 1:4284700/1‑62 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa > 1:2672177/1‑56 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg < 1:3563717/71‑1 (MQ=255) gTGCAGGCCGTCTACTTTAACGTACAGATCTAATTTGTTGCCg > 1:1951569/1‑43 (MQ=255) cAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca < 1:4235278/58‑1 (MQ=255) aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc < 1:482148/71‑1 (MQ=255) ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg < 1:767531/71‑1 (MQ=255) ggCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca < 1:2463377/71‑2 (MQ=12) gTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt < 1:1041773/38‑1 (MQ=255) | TGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCG > minE/1440118‑1440251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |