| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,440,194 | A→G | 14.3% | G36G (GGT→GGC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,440,194 | 0 | A | G | 14.3% | 248.6 / 21.9 | 100 | G36G (GGT→GGC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (46/36); new base G (10/2); total (61/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
AAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAG > minE/1440125‑1440262 | aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTa > 1:3048947/1‑70 (MQ=255)aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:5072382/1‑71 (MQ=255) gACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg > 1:4437036/1‑71 (MQ=255) aCGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg > 1:4789128/1‑70 (MQ=255) cGCATGTATGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa < 1:4228482/71‑1 (MQ=255) cATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACa < 1:5384074/71‑1 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg < 1:4823278/65‑1 (MQ=255) tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa < 1:1611298/71‑1 (MQ=255) tAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc > 1:757451/1‑71 (MQ=255) tAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc > 1:371044/1‑71 (MQ=255) ggTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:243293/1‑59 (MQ=255) ggTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:595585/1‑59 (MQ=255) tCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt < 1:106870/71‑1 (MQ=255) cTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGTCCGTCTACTTTACCGTACAGATCTAAtt > 1:46530/1‑71 (MQ=255) gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCGGTCTACTTTACCGTACAGATCTAAttt < 1:2837429/69‑1 (MQ=255) gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAttt < 1:5245665/69‑1 (MQ=255) gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt < 1:4054538/71‑1 (MQ=255) tCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgtt < 1:3972393/71‑1 (MQ=255) tCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgtt < 1:4415298/71‑1 (MQ=255) ccATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGcc < 1:624218/71‑1 (MQ=255) ccATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGcc > 1:3906280/1‑71 (MQ=255) cATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCg > 1:689984/1‑71 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAg < 1:5167924/53‑1 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAg < 1:5394859/53‑1 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAg < 1:5521815/53‑1 (MQ=255) tCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCAGTCTACTTTACCGTACAGATCTAAtt < 1:3704217/61‑1 (MQ=255) cTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCt > 1:2346539/1‑71 (MQ=255) cTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCt > 1:3663035/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa > 1:4845125/1‑48 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa > 1:78174/1‑48 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:2906427/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:4393193/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:431957/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:2625383/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:1934871/1‑71 (MQ=255) tACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt > 1:3347306/1‑71 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCTTCTttg < 1:180053/71‑1 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCt < 1:2752969/68‑1 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttg < 1:3816773/71‑1 (MQ=255) cATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttg < 1:696502/71‑1 (MQ=255) aTCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg < 1:914361/43‑1 (MQ=255) aTCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCg < 1:4857748/43‑1 (MQ=255) tCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt < 1:780484/54‑1 (MQ=255) tCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt > 1:4338540/1‑58 (MQ=255) cTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc > 1:5208711/1‑39 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa < 1:4519547/70‑1 (MQ=255) tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:2978238/1‑70 (MQ=255) gttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa < 1:3901941/70‑1 (MQ=255) ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATaa > 1:4584766/4‑69 (MQ=2) ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:5075339/4‑71 (MQ=9) tgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt > 1:5530487/1‑64 (MQ=255) atCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATttgt > 1:1072543/2‑50 (MQ=2) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt > 1:323894/1‑70 (MQ=255) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt < 1:2968300/70‑1 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACa > 1:3978740/1‑35 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACa > 1:3062996/1‑35 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt < 1:4981965/43‑1 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt > 1:63663/1‑47 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttgt > 1:1637962/1‑47 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:2106970/1‑71 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:4428153/1‑71 (MQ=255) aaaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAAttt < 1:3358368/41‑1 (MQ=2) gaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagcag > 1:1661661/1‑71 (MQ=255) aaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGt > 1:5335882/2‑50 (MQ=14) aaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcggctg > 1:1430497/2‑69 (MQ=9) aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:3737092/1‑65 (MQ=255) aGTGCAGGCCGTCTACTTTTCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt > 1:1521748/1‑71 (MQ=255) aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca > 1:4284700/1‑62 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa > 1:2672177/1‑56 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg < 1:3563717/71‑1 (MQ=255) gTGCAGGCCGTCTACTTTAACGTACAGATCTAATTTGTTGCCg > 1:1951569/1‑43 (MQ=255) cAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca < 1:4235278/58‑1 (MQ=255) aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc < 1:482148/71‑1 (MQ=255) ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg < 1:767531/71‑1 (MQ=255) ggCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca < 1:2463377/71‑2 (MQ=12) gTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCtt < 1:1041773/38‑1 (MQ=255) tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTg < 1:3581500/70‑1 (MQ=255) tACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc < 1:3016962/69‑1 (MQ=2) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt > 1:605422/1‑39 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca > 1:5107266/1‑49 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:2609717/1‑48 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:3279653/58‑1 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa > 1:1528568/1‑71 (MQ=255) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:4446776/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:5362326/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:1064332/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:1861550/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcg > 1:2065895/2‑48 (MQ=17) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:165270/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:470653/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:2294653/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:1285980/1‑65 (MQ=2) cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4598928/69‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:4836368/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc > 1:4481721/1‑69 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1937160/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:4389517/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1621446/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:520484/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:496206/1‑70 (MQ=255) | AAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAG > minE/1440125‑1440262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |