| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,440,242 | G→A | 16.1% | N20N (AAC→AAT) ‡ | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,440,242 | 0 | G | A | 16.1% | 235.6 / 28.0 | 93 | N20N (AAC→AAT) ‡ | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (46/31); new base A (8/7); total (55/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCC > minE/1440175‑1440312 | aGTGCAGGCCGTCTACTTTTCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt > 1:1521748/1‑71 (MQ=255) gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg < 1:3563717/71‑1 (MQ=255) aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc < 1:482148/71‑1 (MQ=255) ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg < 1:767531/71‑1 (MQ=255) ggCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca < 1:2463377/71‑2 (MQ=12) tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTg < 1:3581500/70‑1 (MQ=255) tACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc < 1:3016962/69‑1 (MQ=2) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:3279653/58‑1 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa > 1:1528568/1‑71 (MQ=255) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:2294653/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:470653/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:165270/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:1285980/1‑65 (MQ=2) cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4598928/69‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:934179/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:4836368/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc > 1:4481721/1‑69 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1621446/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:496206/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:4389517/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:520484/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1937160/1‑70 (MQ=255) ccatACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag < 1:3618084/68‑1 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt > 1:4747511/1‑46 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct < 1:4509361/51‑1 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:2338551/1‑64 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcggc < 1:1619161/61‑4 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag < 1:235588/67‑1 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg < 1:1884941/71‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:3575862/45‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg > 1:4972598/1‑71 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg > 1:1228954/1‑71 (MQ=255) gATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagc < 1:5338854/49‑1 (MQ=16) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCTTTtgc > 1:4884715/1‑45 (MQ=255) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc > 1:1788181/1‑45 (MQ=255) tCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGtt > 1:4738667/1‑41 (MQ=255) cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat > 1:4935129/1‑67 (MQ=255) cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat > 1:2648995/1‑67 (MQ=255) tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt > 1:439235/1‑38 (MQ=255) tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt > 1:1495279/1‑38 (MQ=255) tAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCt < 1:1763009/49‑1 (MQ=12) aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg > 1:5116743/1‑40 (MQ=255) aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2153372/1‑71 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc < 1:788333/67‑1 (MQ=255) aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagcac > 1:1627459/1‑43 (MQ=9) aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca > 1:2573411/1‑43 (MQ=9) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc < 1:5335710/67‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:204270/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:3937048/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2103598/1‑69 (MQ=255) tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg > 1:2438350/1‑36 (MQ=255) tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg > 1:1721441/1‑71 (MQ=255) gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg < 1:3760441/70‑1 (MQ=255) gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg < 1:988937/70‑1 (MQ=255) ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg < 1:75376/71‑1 (MQ=255) gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa > 1:3093427/1‑71 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGCGCTGGGACCAGGAGGGACAGTAc > 1:2184546/1‑71 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa < 1:2971903/55‑1 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc < 1:3814144/57‑1 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:1652277/1‑71 (MQ=255) cTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4306071/39‑1 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag < 1:4770638/57‑1 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:4708676/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:3535897/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:5492746/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:2461263/1‑71 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGATCTGGGACCAGGAGGGACAGTACttt < 1:1410667/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt < 1:169379/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:3792894/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:3094092/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:4949594/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt < 1:3888863/69‑1 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:1156736/1‑36 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:3527272/1‑36 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:4931922/1‑36 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:4123593/1‑63 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:3194175/1‑63 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt > 1:1963810/1‑71 (MQ=255) tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt < 1:314447/71‑1 (MQ=255) tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt > 1:3661286/1‑71 (MQ=255) tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt < 1:649078/70‑1 (MQ=255) aaaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa > 1:1004588/1‑45 (MQ=255) aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGc > 1:5261197/1‑39 (MQ=255) aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc < 1:2271148/70‑1 (MQ=255) tCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt > 1:3377800/1‑70 (MQ=255) cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta > 1:772416/1‑71 (MQ=255) cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt < 1:3587214/69‑1 (MQ=255) cagcagCGTTTGATGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta > 1:3994858/1‑71 (MQ=255) agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat < 1:375657/71‑1 (MQ=255) agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt < 1:2394222/68‑1 (MQ=255) agcGTTTGCTGCGACTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTaa > 1:1496321/1‑71 (MQ=255) gcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAc > 1:296204/1‑71 (MQ=255) gcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACATTAACTTTCATGTTATTAAc > 1:115510/1‑71 (MQ=255) gTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAccc > 1:1424613/1‑71 (MQ=255) | AGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCC > minE/1440175‑1440312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |