| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,440,251 | G→A | 7.8% | G17G (GGC→GGT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,440,251 | 0 | G | A | 7.8% | 208.6 / 6.5 | 77 | G17G (GGC→GGT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (44/27); new base A (2/4); total (46/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTAT > minE/1440181‑1440320 | ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg < 1:767531/71‑1 (MQ=255) tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTg < 1:3581500/70‑1 (MQ=255) tACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc < 1:3016962/69‑1 (MQ=2) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa > 1:1528568/1‑71 (MQ=255) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:2294653/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:1285980/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:470653/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:165270/1‑65 (MQ=2) cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4598928/69‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:934179/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:4836368/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc > 1:4481721/1‑69 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:496206/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1621446/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:4389517/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:520484/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1937160/1‑70 (MQ=255) ccatACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag < 1:3618084/68‑1 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:2338551/1‑64 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcggc < 1:1619161/61‑4 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag < 1:235588/67‑1 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg < 1:1884941/71‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg > 1:4972598/1‑71 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg > 1:1228954/1‑71 (MQ=255) cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat > 1:2648995/1‑67 (MQ=255) cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat > 1:4935129/1‑67 (MQ=255) tAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCt < 1:1763009/49‑1 (MQ=12) aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2153372/1‑71 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc < 1:788333/67‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc < 1:5335710/67‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:3937048/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:204270/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2103598/1‑69 (MQ=255) tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg > 1:1721441/1‑71 (MQ=255) gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg < 1:3760441/70‑1 (MQ=255) gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg < 1:988937/70‑1 (MQ=255) ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg < 1:75376/71‑1 (MQ=255) gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa > 1:3093427/1‑71 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGCGCTGGGACCAGGAGGGACAGTAc > 1:2184546/1‑71 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa < 1:2971903/55‑1 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc < 1:3814144/57‑1 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:1652277/1‑71 (MQ=255) cTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4306071/39‑1 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag < 1:4770638/57‑1 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:4708676/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:3535897/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:5492746/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:2461263/1‑71 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGATCTGGGACCAGGAGGGACAGTACttt < 1:1410667/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:4949594/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:3792894/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt < 1:169379/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:3094092/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt < 1:3888863/69‑1 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:4931922/1‑36 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:3527272/1‑36 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:1156736/1‑36 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:4123593/1‑63 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:3194175/1‑63 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt > 1:1963810/1‑71 (MQ=255) tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt < 1:314447/71‑1 (MQ=255) tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt > 1:3661286/1‑71 (MQ=255) tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt < 1:649078/70‑1 (MQ=255) aaaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa > 1:1004588/1‑45 (MQ=255) aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGc > 1:5261197/1‑39 (MQ=255) aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc < 1:2271148/70‑1 (MQ=255) tCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt > 1:3377800/1‑70 (MQ=255) cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta > 1:772416/1‑71 (MQ=255) cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt < 1:3587214/69‑1 (MQ=255) cagcagCGTTTGATGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta > 1:3994858/1‑71 (MQ=255) agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat < 1:375657/71‑1 (MQ=255) agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt < 1:2394222/68‑1 (MQ=255) agcGTTTGCTGCGACTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTaa > 1:1496321/1‑71 (MQ=255) gcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAc > 1:296204/1‑71 (MQ=255) gcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACATTAACTTTCATGTTATTAAc > 1:115510/1‑71 (MQ=255) gTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAccc > 1:1424613/1‑71 (MQ=255) tgctgcGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctct > 1:372088/1‑71 (MQ=255) ctgcGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGt < 1:1352748/71‑1 (MQ=255) ctgcGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGt < 1:5150770/71‑1 (MQ=255) ctgcGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGt < 1:1788677/71‑1 (MQ=255) gcgcCTGCTACCAGCAGAGCTGGGACCAGTAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGtt < 1:3564345/70‑1 (MQ=255) gcgcCTGCTACCAGCAGAGCTGGGACCAGTAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGtt < 1:3557689/70‑1 (MQ=255) cgcCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTtat < 1:5333586/71‑1 (MQ=255) cgcCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTta > 1:4495536/1‑70 (MQ=255) cgcCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTta > 1:512514/1‑70 (MQ=255) cgcCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTta < 1:90997/70‑1 (MQ=255) cgcCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTta > 1:4810845/1‑70 (MQ=255) cgcCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTta > 1:4489252/1‑70 (MQ=255) | GGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTAT > minE/1440181‑1440320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |