| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,474,863 | C→G | 8.4% | intergenic (+161/‑73) | nrdA → / → nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,474,863 | 0 | C | G | 8.4% | 144.0 / 2.5 | 47 | intergenic (+161/‑73) | nrdA/nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base C (20/23); new base G (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACA > minE/1474794‑1474930 | cGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACgcgc > 1:2051645/1‑70 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa > 1:77001/1‑71 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa > 1:5542865/1‑71 (MQ=255) aTGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt > 1:661098/1‑71 (MQ=255) cAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg > 1:2206749/1‑71 (MQ=255) aGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCAt > 1:5051680/1‑68 (MQ=2) taaaCGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTc > 1:3925360/3‑60 (MQ=2) taaaCGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcat < 1:5142320/69‑2 (MQ=2) gAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg > 1:5173600/1‑71 (MQ=255) gCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg > 1:1205345/1‑71 (MQ=255) ccTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgc > 1:5169314/1‑71 (MQ=255) cTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcg < 1:2114059/71‑1 (MQ=255) tATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCg < 1:2806915/48‑1 (MQ=255) aTCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACgcgc > 1:5502626/1‑42 (MQ=255) aTCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt > 1:862763/1‑53 (MQ=255) aTCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcggcg > 1:302316/1‑71 (MQ=255) aTCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcggcg > 1:4687063/1‑71 (MQ=255) cGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:445863/71‑1 (MQ=255) ccTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAAt < 1:2538107/71‑1 (MQ=255) cTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa < 1:3402006/45‑1 (MQ=255) cTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATg > 1:4818100/1‑71 (MQ=255) tACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa < 1:3046209/44‑1 (MQ=255) tACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAgg < 1:3971003/55‑1 (MQ=255) aaCGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGc < 1:544935/50‑1 (MQ=11) tCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATc > 1:3569158/1‑71 (MQ=255) ggA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATcc < 1:3769936/70‑1 (MQ=255) gTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCAtt > 1:3595493/1‑70 (MQ=255) ggCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTaaa < 1:3489876/70‑1 (MQ=255) ggCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTaaa < 1:2608719/70‑1 (MQ=255) gCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACt > 1:5246982/1‑71 (MQ=255) tGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:4666042/45‑1 (MQ=255) tGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:1805369/45‑1 (MQ=255) tGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:1866718/45‑1 (MQ=255) tGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGcc < 1:3551191/51‑1 (MQ=255) gATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGT‑AAATGCCTTATCCGGCATTAAACTCCCaa > 1:5427689/1‑71 (MQ=255) aTAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGt > 1:5213091/1‑41 (MQ=255) aTAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGcc > 1:4217496/1‑49 (MQ=255) aTAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGcc > 1:2805848/1‑49 (MQ=255) aTAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTc < 1:1711169/67‑1 (MQ=255) aTAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCaa < 1:9259/71‑1 (MQ=255) aTAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCaa > 1:1071461/1‑71 (MQ=255) tAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCaa < 1:1281463/70‑1 (MQ=255) aGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAAc < 1:2226338/69‑1 (MQ=255) aGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAAc < 1:4181682/69‑1 (MQ=255) gACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:3835883/39‑1 (MQ=255) gACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAgg < 1:5164120/71‑1 (MQ=255) aCGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTa > 1:224610/1‑47 (MQ=255) cgcgCCAGCGTCGCATCAGGCACAGGATGCGGCGTaa < 1:5470319/37‑1 (MQ=255) cgcgCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCaa < 1:1491326/65‑1 (MQ=255) gcgcCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGaca < 1:3456300/71‑1 (MQ=255) gcCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAg > 1:3188449/1‑65 (MQ=255) ccAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTa < 1:345226/42‑1 (MQ=255) | CGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACA > minE/1474794‑1474930 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |