| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,502,847 | A→T | 79.3% | intergenic (‑220/‑700) | lrhA ← / → yfbQ | DNA‑binding transcriptional repressor/predicted aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,502,847 | 0 | A | T | 79.3% | 227.1 / 54.1 | 106 | intergenic (‑220/‑700) | lrhA/yfbQ | DNA‑binding transcriptional repressor/predicted aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (13/9); new base T (44/40); total (57/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 107 total aligned reads displayed.
ACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAC > minE/1502779‑1502914 | acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTa > 1:679040/1‑71 (MQ=255) caTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTa < 1:2027626/70‑1 (MQ=255) caTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTa < 1:4137097/70‑1 (MQ=255) caTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTa < 1:3630623/70‑1 (MQ=255) aTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTact > 1:1957182/1‑71 (MQ=255) aTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTact > 1:204538/1‑71 (MQ=255) aTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATact > 1:5044726/1‑71 (MQ=255) ttGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTact > 1:4032057/1‑70 (MQ=255) ttGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTact > 1:5043036/1‑70 (MQ=255) ttGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATact > 1:2955841/1‑70 (MQ=255) ccGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTactcactc < 1:5127489/70‑1 (MQ=255) ccGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCActct > 1:2415717/1‑71 (MQ=255) ccGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATACCTATTAAGTTACTCActct > 1:4829466/1‑71 (MQ=255) cGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCtt < 1:3084535/71‑1 (MQ=255) cGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCtt < 1:4074900/71‑1 (MQ=255) cGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCtt < 1:4313250/71‑1 (MQ=255) ttATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCtttt > 1:2136659/1‑71 (MQ=255) ttATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCttt > 1:656500/1‑70 (MQ=255) ttATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCtttt > 1:673031/1‑71 (MQ=255) tataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTc > 1:2548806/1‑71 (MQ=255) ataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTc < 1:1378624/70‑1 (MQ=255) ataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTc > 1:433649/1‑70 (MQ=255) taATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTa < 1:2128412/71‑1 (MQ=255) ccTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTactcactc < 1:2199723/59‑1 (MQ=255) cTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATg < 1:4301082/71‑1 (MQ=255) tGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTAt < 1:5322005/69‑1 (MQ=255) tGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGa > 1:3402163/1‑71 (MQ=255) gAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGa > 1:2364368/1‑70 (MQ=255) aaGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGa < 1:1823152/69‑1 (MQ=255) gTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCActct > 1:3925618/1‑54 (MQ=255) tAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATgc < 1:3066638/71‑1 (MQ=255) aGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATgcg > 1:736860/1‑71 (MQ=255) gATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATgcg > 1:3795479/1‑70 (MQ=255) gATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATgc > 1:2609518/1‑69 (MQ=255) aTCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTactcac < 1:4806461/47‑1 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtg > 1:4145412/1‑71 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtg > 1:5126153/1‑71 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgt > 1:664600/1‑70 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtgt > 1:2676908/1‑71 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtgt > 1:3041311/1‑71 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtg < 1:5072121/70‑1 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCt < 1:282627/52‑1 (MQ=255) aaTATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGt < 1:2138397/71‑1 (MQ=255) tatatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACa < 1:5251125/56‑1 (MQ=255) tatatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGta > 1:586575/1‑70 (MQ=255) tatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACAt > 1:1416639/1‑55 (MQ=255) tatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACAt > 1:3762275/1‑55 (MQ=255) tatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaa < 1:1399324/71‑1 (MQ=255) tatCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtata > 1:210774/1‑70 (MQ=255) ttCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTAt < 1:4183287/46‑1 (MQ=255) ttCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTAt < 1:2776415/46‑1 (MQ=255) tCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTa > 1:5070670/1‑44 (MQ=255) cAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGta > 1:5609358/1‑62 (MQ=255) cAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgta > 1:3278167/1‑70 (MQ=255) cAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgta < 1:3654242/70‑1 (MQ=255) cAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgta < 1:4622202/70‑1 (MQ=255) cAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgt > 1:5271181/1‑69 (MQ=255) aGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa < 1:1035516/71‑1 (MQ=255) aGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa < 1:4707674/71‑1 (MQ=255) aGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa < 1:1200642/71‑1 (MQ=255) gggATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat < 1:2512015/71‑1 (MQ=255) ggATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtata < 1:393534/61‑1 (MQ=255) ggATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtata < 1:1745828/61‑1 (MQ=255) gATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATaaatgtaaatgt < 1:4612543/71‑1 (MQ=255) aTCGCATATCTATTAAGTTACTCACTCTTTTCTAtt > 1:5002403/1‑36 (MQ=255) aTCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaa > 1:783328/1‑61 (MQ=255) gCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg < 1:106663/70‑1 (MQ=255) gCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGt > 1:5462280/1‑71 (MQ=255) gCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGt > 1:1140723/1‑71 (MQ=255) atatCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTcc < 1:4437583/71‑1 (MQ=255) tatCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaat < 1:4356670/56‑1 (MQ=255) tatCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt < 1:1118158/71‑1 (MQ=255) tatCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa < 1:3622974/61‑1 (MQ=255) tatCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa < 1:3260581/61‑1 (MQ=255) atCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat < 1:108381/61‑1 (MQ=255) atCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat < 1:699857/61‑1 (MQ=255) atCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat < 1:3032185/61‑1 (MQ=255) atCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCtt > 1:3101377/1‑71 (MQ=255) atCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt < 1:1878400/70‑1 (MQ=255) tCTATTAAGTTACTCCCTCTTTTCTATTTATGACATGCGCGTGTTTGt > 1:1270238/1‑48 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTg > 1:5605748/1‑47 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGt > 1:5691474/1‑48 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat > 1:4625501/1‑60 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa > 1:4392262/1‑59 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa > 1:4041565/1‑59 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaa > 1:4664024/1‑59 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTg > 1:5343288/1‑71 (MQ=255) cTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt < 1:2120628/68‑1 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATTCGCGTGTTTGTATAAATGTAAATGTGa > 1:5605045/1‑60 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa > 1:1889717/1‑60 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa > 1:3010775/1‑60 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTc < 1:23599/70‑1 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTc > 1:4311204/1‑70 (MQ=255) ttAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTc < 1:3902110/70‑1 (MQ=255) tAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCa > 1:2642597/1‑71 (MQ=255) aaGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCa < 1:337816/70‑1 (MQ=255) aaGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAc > 1:3595788/1‑71 (MQ=255) aaGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAc > 1:3852191/1‑71 (MQ=255) aaGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTg > 1:4622929/1‑41 (MQ=255) aTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgta < 1:1786311/48‑1 (MQ=255) | ACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAC > minE/1502779‑1502914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |