| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,564,912 | G→A | 76.3% | intergenic (‑124/+168) | yfdE ← / ← glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,564,912 | 0 | G | A | 76.3% | 130.0 / 39.2 | 76 | intergenic (‑124/+168) | yfdE/glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Reads supporting (aligned to +/- strand): ref base G (11/7); new base A (29/29); total (40/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564849‑1564978 | aTAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACa < 1:1394095/68‑1 (MQ=255) aaCATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACaaa > 1:1090873/1‑68 (MQ=255) cATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGcc > 1:3983791/1‑71 (MQ=255) tGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCg > 1:1351107/1‑67 (MQ=255) atcGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc > 1:5665730/3‑69 (MQ=255) atcGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc > 1:13374/3‑69 (MQ=255) gTTAAATCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAAt > 1:989525/1‑57 (MQ=255) ttAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTa < 1:4799263/64‑1 (MQ=255) ttAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGc > 1:2518740/1‑59 (MQ=255) aaTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaaa > 1:745230/1‑71 (MQ=255) aaTTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa > 1:1866222/1‑71 (MQ=255) aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:3780505/71‑1 (MQ=255) aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:620533/71‑1 (MQ=255) aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:4329885/71‑1 (MQ=255) aTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:3513230/71‑1 (MQ=255) ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTa > 1:3833993/1‑58 (MQ=255) ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaa < 1:5404833/66‑1 (MQ=255) ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat > 1:3860437/1‑71 (MQ=255) ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat > 1:1453044/1‑71 (MQ=255) ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa > 1:3845153/1‑70 (MQ=255) ccTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa < 1:4794704/70‑1 (MQ=255) ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat > 1:5530491/1‑71 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:453067/59‑1 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:3466279/59‑1 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:3649770/59‑1 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:1866768/59‑1 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCa < 1:1181267/59‑1 (MQ=255) tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATg > 1:1232916/1‑70 (MQ=255) ttCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:2494953/71‑1 (MQ=255) ttCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:5621617/71‑1 (MQ=255) ttCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:433230/71‑1 (MQ=255) aGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa < 1:2541359/70‑1 (MQ=255) gTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACt < 1:4275528/71‑1 (MQ=255) tGATAGTTGACGAGTGAAATTATGAACGTGCAAAACaaa < 1:2188321/39‑1 (MQ=38) tGATAGTTGACGAGTGAAATTATGAACGTGCAAAACaaa < 1:2050402/39‑1 (MQ=38) gATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg > 1:4525058/1‑70 (MQ=255) aTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGt > 1:4576650/1‑44 (MQ=38) aTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa > 1:1927597/1‑58 (MQ=255) aTAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg > 1:1972136/1‑69 (MQ=255) gttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:210699/67‑1 (MQ=255) gttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:2779091/67‑1 (MQ=255) tgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt > 1:3404159/2‑70 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAttt < 1:5086357/59‑1 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt > 1:2494595/1‑70 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTa < 1:3564703/71‑1 (MQ=255) gacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt > 1:4805922/1‑70 (MQ=255) acgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa > 1:2720629/1‑71 (MQ=255) acgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa < 1:2357501/71‑1 (MQ=255) acgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa < 1:934415/71‑1 (MQ=255) ctaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:2761392/63‑1 (MQ=255) tGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata > 1:5284013/1‑45 (MQ=255) tGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCg > 1:5608278/1‑71 (MQ=255) gAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGc > 1:3449051/1‑71 (MQ=255) aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat > 1:238978/1‑42 (MQ=255) aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:1245752/1‑71 (MQ=255) aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataa > 1:5503957/1‑44 (MQ=255) aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa > 1:1244948/1‑52 (MQ=255) aTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgaga < 1:4067804/57‑1 (MQ=255) aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata > 1:2916374/1‑41 (MQ=255) aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata > 1:3826324/1‑41 (MQ=255) aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg > 1:4397406/1‑70 (MQ=255) tATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:4301292/49‑1 (MQ=255) tATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:5383225/49‑1 (MQ=255) tATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:4165229/49‑1 (MQ=255) aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa > 1:3071688/1‑47 (MQ=255) aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTa > 1:3426928/1‑47 (MQ=255) aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:906641/1‑66 (MQ=255) aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:2168664/1‑66 (MQ=255) aTGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:3120076/1‑66 (MQ=255) tGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg > 1:4545645/1‑49 (MQ=255) tGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg < 1:5235651/71‑1 (MQ=255) gAACGTGCAAAACAAATCGCCGTTACCAGACAaataa < 1:337291/37‑1 (MQ=255) aCGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg > 1:5247916/1‑71 (MQ=255) aCGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg < 1:678046/71‑1 (MQ=255) gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:4726409/70‑1 (MQ=255) gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:3085498/70‑1 (MQ=255) | ATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564849‑1564978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |