| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,591,621 | G→A | 48.9% | intergenic (+80/+109) | yfeS → / ← cysM | conserved hypothetical protein/cysteine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,591,621 | 0 | G | A | 48.9% | 6.5 / 177.0 | 133 | intergenic (+80/+109) | yfeS/cysM | conserved hypothetical protein/cysteine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (36/32); new base A (35/30); total (71/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 133 total aligned reads displayed.
TGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCA > minE/1591552‑1591687 | ggCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:4785641/71‑1 (MQ=255) ctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTa < 1:41480‑M1/31‑1 (MQ=255) ctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTa < 1:2746008‑M1/31‑1 (MQ=255) ctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc > 1:2185675‑M1/40‑71 (MQ=255) tgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc < 1:2792159‑M1/32‑1 (MQ=255) tgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTAc > 1:4427802‑M1/39‑70 (MQ=255) gataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACa < 1:1148611‑M1/33‑1 (MQ=255) gataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACa < 1:2192035‑M1/33‑1 (MQ=255) gataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa < 1:653082‑M1/33‑1 (MQ=255) ctgataagacggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa < 1:1112508‑M1/33‑1 (MQ=255) ataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaa > 1:224601‑M1/37‑71 (MQ=255) ataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaa < 1:1293882‑M1/34‑1 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTGTCCGACCTACa > 1:1598038‑M1/37‑69 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACaa < 1:4663289‑M1/34‑1 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa > 1:3133308‑M1/37‑69 (MQ=255) ataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACa > 1:3968541‑M1/37‑69 (MQ=255) taagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaaa > 1:2930468‑M1/36‑71 (MQ=255) aagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaaa < 1:3316933‑M1/36‑1 (MQ=255) aagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAt < 1:3698561‑M1/37‑1 (MQ=255) aagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAt < 1:1652032‑M1/37‑1 (MQ=255) aagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAt < 1:5355782‑M1/37‑1 (MQ=255) aagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAt < 1:4347264‑M1/37‑1 (MQ=255) aagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAt < 1:701828‑M1/37‑1 (MQ=255) aacaacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATc > 1:4471248/1‑71 (MQ=255) aacaacTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATc > 1:1993540/1‑71 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAtca < 1:2919820‑M1/39‑2 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAtca > 1:1472609‑M1/33‑70 (MQ=255) gacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAAtca < 1:2232205‑M1/39‑2 (MQ=255) ttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACaaaa > 1:670278‑M1/27‑62 (MQ=255) ttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTaa > 1:4081563‑M1/27‑71 (MQ=255) gTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTaa > 1:3292707/1‑71 (MQ=255) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAt < 1:2143460/62‑1 (MQ=39) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTaa < 1:2770226/70‑1 (MQ=255) ttAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAAt > 1:4599416/1‑71 (MQ=255) caagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:209120‑M1/26‑70 (MQ=255) caagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCtaaa < 1:4873076‑M1/46‑2 (MQ=255) tAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAAtt > 1:2474244/1‑71 (MQ=40) tAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAAtt > 1:2591310/1‑71 (MQ=40) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:2920001‑M1/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:3968611‑M1/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:2446982‑M1/25‑69 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCtaaa < 1:2228188‑M1/46‑2 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACgctaaatc > 1:4238166‑M1/24‑68 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat < 1:2089850‑M1/47‑3 (MQ=255) agcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat < 1:2082957‑M1/47‑3 (MQ=255) gcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATCa > 1:655331‑M1/23‑71 (MQ=255) gcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATCa > 1:4811518‑M1/23‑71 (MQ=255) aaaaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCa < 1:2427778/71‑1 (MQ=38) aaaaaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCa < 1:3609408/71‑1 (MQ=38) cgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag > 1:5546817‑M1/22‑70 (MQ=255) cgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag > 1:187267‑M1/22‑70 (MQ=255) cgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:519117‑M1/50‑2 (MQ=255) gtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAAtcagg < 1:1299606‑M1/51‑3 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:3384337‑M1/20‑64 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAatcaggc > 1:3504489‑M1/20‑68 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACGTACAAAATCACGCTAAatcaggc < 1:3454996‑M1/52‑4 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCtaaa > 1:462860‑M1/20‑64 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAatcaggc > 1:4696563‑M1/20‑68 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAatcagga > 1:1080715‑M1/20‑68 (MQ=255) cgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaat > 1:754138‑M1/19‑63 (MQ=255) gTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAatata < 1:3988206/71‑1 (MQ=39) tGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAatatat < 1:2293409/71‑1 (MQ=39) catcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATCa < 1:3876066‑M1/49‑1 (MQ=255) gcgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATAtt > 1:1995596/1‑71 (MQ=39) gcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag > 1:3826488‑M1/11‑59 (MQ=255) gTTTATGCCGGATTCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTc < 1:3353321/56‑1 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTc < 1:2608126/56‑1 (MQ=35) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCt > 1:2820729/1‑71 (MQ=255) tATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGaaatca > 1:305977/1‑68 (MQ=11) tATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGaaatc < 1:4377490/70‑3 (MQ=11) gatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:228643‑M1/50‑2 (MQ=255) gatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACGCTAAATcag < 1:4317859‑M1/50‑2 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGc > 1:2859712/1‑71 (MQ=11) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCt < 1:321210/41‑1 (MQ=32) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAgg > 1:161687/1‑70 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGc > 1:3033291/1‑71 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGc < 1:487477/71‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGc > 1:1538705/1‑55 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCt < 1:4258487/71‑1 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTa > 1:4840718/1‑65 (MQ=11) cggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAc > 1:5689638/1‑58 (MQ=255) gTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAg > 1:1172367/1‑71 (MQ=255) tAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGaaat < 1:5390949/53‑2 (MQ=11) tAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGaaat < 1:3676233/53‑2 (MQ=11) tAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGCCt < 1:3975788/64‑1 (MQ=11) tAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCa < 1:750435/38‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAg < 1:579092/70‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCTAATTCaa > 1:2654557/1‑37 (MQ=40) aaCGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCg > 1:3221703/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCg > 1:3680747/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg > 1:2013041/1‑47 (MQ=11) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg > 1:2702139/1‑47 (MQ=11) gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaatt < 1:4338094/48‑3 (MQ=11) cTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAgcg > 1:234498/1‑70 (MQ=255) attATCCGGCCTACAAAATCGTGCTAATTCAATATATTGGAGAAACCTTg < 1:4279856/49‑1 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTa > 1:3572312/1‑50 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1725128/1‑71 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1676644/1‑71 (MQ=255) aTCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCtt < 1:136632/46‑1 (MQ=255) aTCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1384668/1‑70 (MQ=255) | TGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCA > minE/1591552‑1591687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |