| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,602,585 | T→C | 22.1% | intergenic (+121/+119) | yfeW → / ← yfeX | predicted periplasmic esterase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,602,585 | 0 | T | C | 22.1% | 241.1 / 67.4 | 131 | intergenic (+121/+119) | yfeW/yfeX | predicted periplasmic esterase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (46/56); new base C (18/11); total (64/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01 | |||||||||||
Only 100 of 138 total aligned reads displayed.
CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGG > minE/1602515‑1602654 | gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAttt < 1:1279961/71‑1 (MQ=255) gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAttt < 1:2185713/71‑1 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCg < 1:2659121/71‑1 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCg < 1:3419870/71‑1 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCg < 1:1513567/71‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAttt > 1:867772/1‑68 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGAtt > 1:1973801/1‑65 (MQ=255) cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAgg > 1:5153268/1‑71 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc < 1:641564/70‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCg < 1:2982001/71‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGAt < 1:2846181/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGAt < 1:614292/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATa < 1:5031299/71‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATa < 1:1430137/70‑1 (MQ=255) gtggcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGc > 1:3298991‑M1/53‑71 (MQ=255) tgtcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCg > 1:1139451‑M1/52‑71 (MQ=255) tggcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCg > 1:2012125‑M1/52‑71 (MQ=255) gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCg > 1:2927651‑M1/50‑53 (MQ=255) gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:3622469‑M1/6‑1 (MQ=255) gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGtt < 1:3459302‑M1/22‑1 (MQ=255) acaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATa > 1:2427606‑M1/50‑64 (MQ=255) aCGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGc > 1:616019/1‑58 (MQ=255) cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGc < 1:2024242/57‑1 (MQ=255) cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGc < 1:4181072/57‑1 (MQ=255) cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt < 1:3969903/70‑1 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCa < 1:4205089‑M1/24‑1 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGgcgtcca < 1:4713640‑M1/24‑4 (MQ=255) cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc > 1:4264186/1‑56 (MQ=255) cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCg > 1:3117800/1‑57 (MQ=255) atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCTTTCAcg > 1:9523‑M1/46‑71 (MQ=255) atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAc > 1:2198300‑M1/46‑70 (MQ=255) gcctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATaa < 1:545159‑M1/16‑1 (MQ=255) tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:4105072/1‑71 (MQ=255) cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:4313221‑M1/43‑71 (MQ=255) cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:5410137‑M1/43‑71 (MQ=255) cAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:3341650/1‑70 (MQ=255) cAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:4164196/1‑70 (MQ=255) tgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:921575‑M1/6‑1 (MQ=255) ctgatgcgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCa > 1:4208134‑M1/41‑71 (MQ=255) aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccgc < 1:5149331/70‑1 (MQ=255) gatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3601042‑M1/32‑1 (MQ=255) gatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3279022‑M1/32‑1 (MQ=255) atgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1533701‑M1/39‑71 (MQ=255) atgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCa > 1:884991‑M1/39‑69 (MQ=255) atgcgctacgcttatcaggcctacaattctcgcgccaCTCGTa < 1:2265395‑M1/6‑1 (MQ=255) gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:3864489‑M1/6‑1 (MQ=255) gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:1525568‑M1/6‑1 (MQ=255) gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:2108340‑M1/6‑1 (MQ=255) gcgctacgcttatcaggcctacagattcttgcgccaTTCGTa < 1:535221‑M1/6‑1 (MQ=255) gcgctacgcttatcaggcctacagattctcgcgccaCTCg > 1:1097183‑M1/37‑40 (MQ=255) gcgctacgcttatcaggcctacagattctcgcgccaCTCg > 1:4861843‑M1/37‑40 (MQ=255) gcgctacgcttatcaggcctacagattctcgcgccaCTCg > 1:1371043‑M1/37‑40 (MQ=255) gcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:534969‑M1/35‑1 (MQ=255) tACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt < 1:2807604/57‑1 (MQ=255) tACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4429880/1‑71 (MQ=255) cgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:3979161‑M1/36‑1 (MQ=255) gctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:5620472‑M1/34‑70 (MQ=255) ctacgcttatcaggcctacagattcttgcgccaTTc > 1:2081101‑M1/34‑36 (MQ=255) ctacgcttatcaggcctacagattcttgcgccaTTc > 1:2413192‑M1/34‑36 (MQ=255) cGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:2535100/1‑57 (MQ=255) cGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4481191/1‑69 (MQ=255) tacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCg > 1:5606946‑M1/33‑43 (MQ=255) tGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtg < 1:536118/71‑1 (MQ=255) cgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGgca < 1:73329‑M1/38‑2 (MQ=255) gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtgt > 1:2940791/1‑70 (MQ=255) tatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAcat < 1:768055‑M1/45‑2 (MQ=255) tatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATcc > 1:1181507‑M1/27‑60 (MQ=255) tatcaggcctacaaattcccgcgccaTCCGTAGGCCGGATAAGGCGTTCAcgccgc > 1:2548375‑M1/27‑56 (MQ=255) atcaggcctacagattcttgcgccaTTCGTAGGCCgg < 1:4443203‑M1/12‑1 (MQ=255) tcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAacatg > 1:769987‑M1/25‑68 (MQ=255) tcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:4204713‑M1/35‑1 (MQ=255) tcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:2144493‑M1/35‑1 (MQ=255) tcaggcctacaaattcccgcaccaTCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:4522534‑M1/25‑61 (MQ=255) cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:2953306/44‑1 (MQ=255) cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:3730246/44‑1 (MQ=255) cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:5560262/44‑1 (MQ=255) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:4705983/42‑1 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCg < 1:2927814/42‑1 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:5003444/1‑44 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:1857770/1‑44 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:841473/1‑44 (MQ=38) atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:2608166/1‑43 (MQ=255) tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:2734752/1‑42 (MQ=255) tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCAc > 1:5068593/1‑71 (MQ=255) tatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:1222205/1‑41 (MQ=255) tatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:1007690/48‑1 (MQ=255) tacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATc > 1:2597924‑M1/18‑50 (MQ=255) atTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgc < 1:195599/43‑1 (MQ=255) ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:801883/1‑50 (MQ=255) ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:280150/1‑50 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCa < 1:3717949‑M1/24‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:4790849‑M1/31‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:315120‑M1/31‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:3579973‑M1/31‑1 (MQ=255) gattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAcgccg < 1:870899‑M1/29‑1 (MQ=255) gAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:1994417/1‑37 (MQ=255) ttctcacgccaTCCGTAGGCCGGATAAGGCGTTCAcgcc < 1:2525628‑M1/28‑1 (MQ=255) ttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:2060699‑M1/31‑1 (MQ=255) aCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCt > 1:2755046/1‑71 (MQ=255) tttCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAgg < 1:2886075/71‑1 (MQ=255) | CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGG > minE/1602515‑1602654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |