Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 309,836 T→A 31.8% T1013S (ACC→TCC) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE309,8360TA31.8% 107.1 / 66.2 79T1013S (ACC→TCC) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base A (11/14);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAA  >  minE/309768‑309904
                                                                    |                                                                    
gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt                                                                      <  1:1037312/69‑1 (MQ=255)
gCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGt                                                                      <  1:4802089/69‑1 (MQ=255)
    ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                                >  1:1446701/2‑71 (MQ=16)
    ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                                >  1:221571/2‑71 (MQ=16)
     tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                                <  1:701302/70‑1 (MQ=255)
     tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                                <  1:1254904/70‑1 (MQ=255)
     tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt                                                                <  1:2154671/70‑1 (MQ=255)
      aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc                                                              >  1:3255651/1‑71 (MQ=255)
       aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                               <  1:3879147/69‑1 (MQ=255)
       aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                               <  1:3234260/69‑1 (MQ=255)
       aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                               <  1:2545637/69‑1 (MQ=255)
        aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg                                                            >  1:3422099/1‑71 (MQ=255)
          cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTCCAGTACCGTTGCGGTCACCATcc                                                              >  1:4777737/1‑66 (MQ=255)
           ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                          <  1:3265306/70‑1 (MQ=255)
                cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                     <  1:4164197/70‑1 (MQ=255)
                   accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc                                                 >  1:4442604/1‑71 (MQ=255)
                    tcaccacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTAcccc                                                 >  1:314986/2‑70 (MQ=255)
                    ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa                                                        >  1:5409611/1‑63 (MQ=255)
                      accacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                          <  1:3281504/59‑1 (MQ=255)
                      accacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTaccc                                                  >  1:5354213/1‑65 (MQ=16)
                        cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                          <  1:1440765/57‑1 (MQ=255)
                        cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa                                                        >  1:4718558/1‑59 (MQ=255)
                        cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                                            >  1:4126667/1‑71 (MQ=255)
                         acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc                                           >  1:5554540/1‑71 (MQ=255)
                          caAAGAATACCGGAACTAAGAAGATTGCCAGTACCGTTGCGGTCAc                                                                   <  1:1065477/46‑1 (MQ=255)
                          caAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTTCGGTCAc                                                                   <  1:4336929/46‑1 (MQ=255)
                          caAAGAATACCGGAACCAAGAAGATTGCCAGGACCGTTGCGGCCAc                                                                   <  1:5163425/46‑1 (MQ=255)
                            aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                         >  1:5162098/1‑70 (MQ=255)
                            aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                         >  1:849491/1‑68 (MQ=12)
                             aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                           <  1:625993/67‑1 (MQ=255)
                             aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                           <  1:847248/67‑1 (MQ=255)
                             aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                           <  1:182003/67‑1 (MQ=255)
                             aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                          <  1:973145/52‑1 (MQ=12)
                             aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                          <  1:31127/52‑1 (MQ=12)
                              gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag                                      >  1:2923753/1‑69 (MQ=255)
                                acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                    >  1:4712527/3‑71 (MQ=255)
                                acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                    >  1:3298615/3‑71 (MQ=255)
                                 tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                         >  1:5670355/1‑65 (MQ=255)
                                 tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                         >  1:2475731/1‑65 (MQ=255)
                                  aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                  <  1:5275557/71‑1 (MQ=255)
                                   ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac                                                    <  1:878289/52‑1 (MQ=255)
                                    cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                         >  1:5372972/1‑60 (MQ=255)
                                     ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac                                                    <  1:5521459/50‑1 (MQ=255)
                                     ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                               >  1:309971/1‑71 (MQ=255)
                                     ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                               <  1:2365160/71‑1 (MQ=255)
                                      gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                              <  1:2910880/69‑1 (MQ=255)
                                      gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                              <  1:2736173/69‑1 (MQ=255)
                                      gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc                              <  1:2729606/71‑1 (MQ=255)
                                       aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac                                                  >  1:5452146/1‑50 (MQ=255)
                                       aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac                                                  >  1:3542944/1‑50 (MQ=255)
                                        aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                            >  1:2623255/1‑71 (MQ=255)
                                         cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg                           >  1:2352911/1‑71 (MQ=255)
                                         cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                  >  1:1336105/1‑64 (MQ=255)
                                         cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                  >  1:487488/1‑64 (MQ=255)
                                          gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                                       <  1:5150395/42‑1 (MQ=255)
                                          gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                          <  1:4858816/71‑1 (MQ=255)
                                          gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc                          <  1:1760126/71‑1 (MQ=12)
                                           aagaagATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                                       <  1:3118294/41‑1 (MQ=255)
                                           aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                  <  1:4603109/62‑1 (MQ=255)
                                           aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                  <  1:2606199/62‑1 (MQ=255)
                                           aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                  <  1:1588986/62‑1 (MQ=255)
                                             gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                       <  1:1517037/71‑1 (MQ=255)
                                             gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                       <  1:1306413/71‑1 (MQ=255)
                                              aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa                      <  1:448472/71‑1 (MQ=255)
                                                gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                    >  1:3216696/1‑71 (MQ=255)
                                                gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                    <  1:91297/71‑1 (MQ=255)
                                                gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                    >  1:3420077/1‑71 (MQ=255)
                                                 aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                    <  1:2801117/70‑1 (MQ=255)
                                                 aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                           <  1:4018313/63‑1 (MQ=255)
                                                 aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                           <  1:4069408/63‑1 (MQ=255)
                                                    gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                 >  1:4999943/1‑70 (MQ=255)
                                                         tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg           >  1:1582566/1‑71 (MQ=255)
                                                          aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg           >  1:5516986/1‑70 (MQ=255)
                                                           ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa         >  1:2665121/1‑71 (MQ=255)
                                                             gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt       >  1:1012766/1‑71 (MQ=255)
                                                              ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTCTGCGCGCCGGAACCAGCACCAGTACt       >  1:4175240/1‑70 (MQ=255)
                                                                 cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt    <  1:468344/70‑1 (MQ=255)
                                                                   gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                   <  1:4097617/37‑1 (MQ=255)
                                                                   gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                   <  1:4352798/37‑1 (MQ=255)
                                                                   gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                   <  1:5169313/37‑1 (MQ=255)
                                                                   gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa  <  1:2850347/70‑1 (MQ=255)
                                                                    tCACCATCCCGCCCATTACACCGGTACCTACTGCg                                    <  1:719907/35‑1 (MQ=255)
                                                                    |                                                                    
GCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAA  >  minE/309768‑309904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: