| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,602,606 | T→C | 15.2% | intergenic (+142/+98) | yfeW → / ← yfeX | predicted periplasmic esterase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,602,606 | 0 | T | C | 15.2% | 210.3 / 27.5 | 92 | intergenic (+142/+98) | yfeW/yfeX | predicted periplasmic esterase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (38/40); new base C (8/6); total (46/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
ACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGC > minE/1602536‑1602674 | gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGtt < 1:2019165‑M1/22‑1 (MQ=255)gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGtt < 1:3459302‑M1/22‑1 (MQ=255) cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt < 1:3969903/70‑1 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCa < 1:4205089‑M1/24‑1 (MQ=255) atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCTTTCAcg > 1:9523‑M1/46‑71 (MQ=255) atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAcg > 1:2084738‑M1/46‑71 (MQ=255) atgcctgatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAc > 1:2198300‑M1/46‑70 (MQ=255) atgcctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcg < 1:5272066‑M1/26‑1 (MQ=255) tgcctgatgcgctacgcttatcaggcctaaagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAcgc < 1:711982‑M1/27‑1 (MQ=255) tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:4105072/1‑71 (MQ=255) cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:4313221‑M1/43‑71 (MQ=255) cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:5410137‑M1/43‑71 (MQ=255) cctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccg > 1:77811‑M1/43‑71 (MQ=255) ctgatgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCAcgccgc > 1:2867373‑M1/42‑71 (MQ=255) cAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:4164196/1‑70 (MQ=255) cAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccg > 1:3341650/1‑70 (MQ=255) ctgatgcgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCa > 1:4208134‑M1/41‑71 (MQ=255) ctgatgcgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCa > 1:4201390‑M1/41‑71 (MQ=255) aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccgc < 1:5149331/70‑1 (MQ=255) gatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3279022‑M1/32‑1 (MQ=255) gatgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3601042‑M1/32‑1 (MQ=255) atgcgctacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1533701‑M1/39‑71 (MQ=255) atgcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCa > 1:884991‑M1/39‑69 (MQ=255) ccTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:5105273/1‑71 (MQ=255) gcgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:534969‑M1/35‑1 (MQ=255) tACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt < 1:2807604/57‑1 (MQ=255) tACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4429880/1‑71 (MQ=255) cgctacgcttatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCgg > 1:3046333‑M1/36‑71 (MQ=255) cgctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:3979161‑M1/36‑1 (MQ=255) gctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGgca > 1:2570550‑M1/34‑70 (MQ=255) gctacgcttatcaggcctacaattctcgcgccaCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:5620472‑M1/34‑70 (MQ=255) cGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:2535100/1‑57 (MQ=255) cGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4481191/1‑69 (MQ=255) tacgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcaa < 1:4788693‑M1/39‑3 (MQ=255) tGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtg < 1:536118/71‑1 (MQ=255) tGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtg < 1:5593013/71‑1 (MQ=255) cgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGgca < 1:73329‑M1/38‑2 (MQ=255) cgcttatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc < 1:3955404‑M1/37‑1 (MQ=255) gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtgt > 1:2940791/1‑70 (MQ=255) ttatcaggcctacaaattcccgcgccaTCCGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:2807260‑M1/33‑1 (MQ=255) ttatcagacctacaaattctcacgccaTCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:3392862‑M1/28‑63 (MQ=255) tatcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAcat < 1:768055‑M1/45‑2 (MQ=255) tatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATcc > 1:1181507‑M1/27‑60 (MQ=255) tatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCGgca < 1:1179752‑M1/38‑2 (MQ=255) tatcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCGgca < 1:591948‑M1/38‑2 (MQ=255) tatcaggcctacaaattcccgcgccaTCCGTAGGCCGGATAAGGCGTTCAcgccgc > 1:2548375‑M1/27‑56 (MQ=255) tcaggcctacagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAacatg > 1:769987‑M1/25‑68 (MQ=255) tcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:4204713‑M1/35‑1 (MQ=255) tcaggcctacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCg < 1:2144493‑M1/35‑1 (MQ=255) tcaggcctacaaattcccgcaccaTCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:4522534‑M1/25‑61 (MQ=255) tcaggcctacaaattcccgcaccaTCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc > 1:3267046‑M1/25‑61 (MQ=255) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:1857770/1‑44 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:4214999/1‑44 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:841473/1‑44 (MQ=38) caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGtt > 1:5003444/1‑44 (MQ=38) atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:2608166/1‑43 (MQ=255) tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:2734752/1‑42 (MQ=255) tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCAc > 1:5068593/1‑71 (MQ=255) ctacaaattctcacgccaTCCGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:1625640‑M1/33‑1 (MQ=255) tatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:1222205/1‑41 (MQ=255) tatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:1007690/48‑1 (MQ=255) tacagattctcgcgccaCTCGTAGGCCGGATAAGGCGTCCACGCCGCATc > 1:2597924‑M1/18‑50 (MQ=255) tacaaattctcgcgccaCCCGTAGGCCGGATAAGGCGTTCAcgccgc > 1:5399880‑M1/18‑47 (MQ=255) atTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgc < 1:195599/43‑1 (MQ=255) ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:801883/1‑50 (MQ=255) ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:280150/1‑50 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCa < 1:3717949‑M1/24‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:315120‑M1/31‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:4790849‑M1/31‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:4403124‑M1/33‑1 (MQ=255) cagattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCAACCggcaa < 1:2715978‑M1/39‑3 (MQ=255) gattcttgcgccaTTCGTAGGCCGGATAAGGCGTTCAcgccg < 1:870899‑M1/29‑1 (MQ=255) gAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTc > 1:3506159/1‑36 (MQ=255) gAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa > 1:1994417/1‑37 (MQ=255) ttctcacgccaTCCGTAGGCCGGATAAGGCGTTCAcgcc < 1:2525628‑M1/28‑1 (MQ=255) ttctcacgccaTCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:4501568‑M1/12‑47 (MQ=255) ttgtgccaTTCGTAGGCCGGATAAGGCGTTCAcgccgc < 1:1444150‑M1/30‑1 (MQ=255) ttgcgccaTTCGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:2060699‑M1/31‑1 (MQ=255) aCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCt > 1:2755046/1‑71 (MQ=255) gATTTCGTAGTCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCt > 1:2185763/1‑69 (MQ=255) tttCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAgg < 1:2886075/71‑1 (MQ=255) tCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGc < 1:5068809/70‑1 (MQ=255) tCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGc > 1:1406879/1‑70 (MQ=255) cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGcc < 1:2489035/70‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcagt > 1:5048955/1‑69 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcagt > 1:1982972/1‑69 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCt < 1:4444136/57‑1 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTa < 1:2578570/71‑1 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTaa < 1:526786/71‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATg < 1:330856/71‑1 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCAc < 1:4952622/39‑1 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGt < 1:1501737/71‑1 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGt > 1:3374618/1‑71 (MQ=255) aGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGtt < 1:874546/70‑1 (MQ=255) ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGAcagtcagt < 1:950834/63‑1 (MQ=255) gCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTaa > 1:1533119/1‑64 (MQ=255) cGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTg < 1:1285640/71‑1 (MQ=255) gTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGc < 1:1894343/71‑1 (MQ=255) ttCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTcaacaa < 1:3889928/43‑1 (MQ=255) ttCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTcaaca < 1:2062302/42‑1 (MQ=255) | ACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGC > minE/1602536‑1602674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |