| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,837 | C→G | 31.2% | V1012V (GTG→GTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,837 | 0 | C | G | 31.2% | 110.9 / 63.4 | 80 | V1012V (GTG→GTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (24/31); new base G (11/14); total (35/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAA > minE/309772‑309904 | ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:1446701/2‑71 (MQ=16)ttAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt > 1:221571/2‑71 (MQ=16) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:701302/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:1254904/70‑1 (MQ=255) tAAAGCAACGGCGGATCACCACAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCAt < 1:2154671/70‑1 (MQ=255) aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc > 1:3255651/1‑71 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3234260/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:2545637/69‑1 (MQ=255) aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc < 1:3879147/69‑1 (MQ=255) aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg > 1:3422099/1‑71 (MQ=255) cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATT‑CCAGTACCGTTGCGGTCACCATcc > 1:4777737/1‑66 (MQ=255) ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:3265306/70‑1 (MQ=255) cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa < 1:4164197/70‑1 (MQ=255) accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc > 1:4442604/1‑71 (MQ=255) tcaccacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTAcccc > 1:314986/2‑70 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa > 1:5409611/1‑63 (MQ=255) accacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:3281504/59‑1 (MQ=255) accacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTaccc > 1:5354213/1‑65 (MQ=16) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc < 1:1440765/57‑1 (MQ=255) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa > 1:4718558/1‑59 (MQ=255) cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc > 1:4126667/1‑71 (MQ=255) acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc > 1:5554540/1‑71 (MQ=255) caAAGAATACCGGAACTAAGAAGATTGCCAGTACCGTTGCGGTCAc < 1:1065477/46‑1 (MQ=255) caAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTTCGGTCAc < 1:4336929/46‑1 (MQ=255) caAAGAATACCGGAACCAAGAAGATTGCCAGGACCGTTGCGGCCAc < 1:5163425/46‑1 (MQ=255) aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:5162098/1‑70 (MQ=255) aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca > 1:849491/1‑68 (MQ=12) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:182003/67‑1 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:625993/67‑1 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:847248/67‑1 (MQ=255) aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc < 1:973145/52‑1 (MQ=12) aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc < 1:31127/52‑1 (MQ=12) gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag > 1:2923753/1‑69 (MQ=255) acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg > 1:4712527/3‑71 (MQ=255) acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg > 1:3298615/3‑71 (MQ=255) tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:5670355/1‑65 (MQ=255) tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:2475731/1‑65 (MQ=255) aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:5275557/71‑1 (MQ=255) ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac < 1:878289/52‑1 (MQ=255) cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca > 1:5372972/1‑60 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac < 1:5521459/50‑1 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg < 1:2365160/71‑1 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg > 1:309971/1‑71 (MQ=255) gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc < 1:2736173/69‑1 (MQ=255) gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc < 1:2910880/69‑1 (MQ=255) gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc < 1:2729606/71‑1 (MQ=255) aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac > 1:5452146/1‑50 (MQ=255) aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac > 1:3542944/1‑50 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc > 1:2623255/1‑71 (MQ=255) cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg > 1:2352911/1‑71 (MQ=255) cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt > 1:1336105/1‑64 (MQ=255) cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt > 1:487488/1‑64 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt < 1:5150395/42‑1 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc < 1:4858816/71‑1 (MQ=255) gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc < 1:1760126/71‑1 (MQ=12) aagaagATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt < 1:3118294/41‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:4603109/62‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:1588986/62‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:2606199/62‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1517037/71‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1306413/71‑1 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa < 1:448472/71‑1 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:91297/71‑1 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc > 1:3216696/1‑71 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc > 1:3420077/1‑71 (MQ=255) aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:2801117/70‑1 (MQ=255) aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg < 1:4018313/63‑1 (MQ=255) aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg < 1:4069408/63‑1 (MQ=255) gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:4999943/1‑70 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:1582566/1‑71 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:5516986/1‑70 (MQ=255) ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa > 1:2665121/1‑71 (MQ=255) gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt > 1:1012766/1‑71 (MQ=255) ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTCTGCGCGCCGGAACCAGCACCAGTACt > 1:4175240/1‑70 (MQ=255) cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt < 1:468344/70‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:4097617/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:4352798/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:5169313/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa < 1:2850347/70‑1 (MQ=255) tCACCATCCCGCCCATTACACCGGTACCTACTGCg < 1:719907/35‑1 (MQ=255) | CTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAA > minE/309772‑309904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |