| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,466 | G→A | 42.9% | intergenic (‑91/+126) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,466 | 0 | G | A | 42.9% | 91.4 / 101.7 | 91 | intergenic (‑91/+126) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (33/19); new base A (15/24); total (48/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
TTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGC > minE/1614398‑1614535 | ttatttatTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc > 1:3972471/1‑71 (MQ=255) atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcgc > 1:1815634/1‑71 (MQ=255) atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcg < 1:5531776/70‑1 (MQ=255) ttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCgg > 1:2696822/1‑71 (MQ=255) ttGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGc > 1:3541707/1‑69 (MQ=255) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCa < 1:2234559/69‑1 (MQ=11) tGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:1456548/1‑71 (MQ=39) tGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTAGATAAGACGCGGCAAg > 1:4947173/1‑71 (MQ=255) gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCg > 1:3857576/1‑71 (MQ=14) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGttg > 1:1982493/1‑68 (MQ=14) tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCa < 1:3901698/71‑1 (MQ=14) tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:2416348/71‑1 (MQ=39) tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:3641343/71‑1 (MQ=39) tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:4221789/71‑1 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATc > 1:2534512/1‑71 (MQ=14) ggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATcc < 1:3777624/71‑1 (MQ=39) ggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATcc < 1:2929521/71‑1 (MQ=39) cgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCgg > 1:2523114/1‑71 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:4820010/71‑1 (MQ=14) tAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGttg < 1:1639171/62‑3 (MQ=14) tAAACGCCTTATCCGCACTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCa < 1:4381879/71‑1 (MQ=11) aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCaa > 1:1555259/1‑71 (MQ=38) gaCGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGcgt < 1:5543437/69‑3 (MQ=11) aaCGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTc > 1:1030981/1‑59 (MQ=11) aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:2938918/1‑70 (MQ=11) aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:3491071/1‑70 (MQ=11) aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:671011/1‑70 (MQ=11) aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:3353167/1‑70 (MQ=11) aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:658721/1‑70 (MQ=11) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3856953/1‑71 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:2797065/1‑71 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:1797315/1‑71 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt < 1:5201586/71‑1 (MQ=38) aCGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCatct > 1:1726505/1‑68 (MQ=11) gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:38098/1‑70 (MQ=11) gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:5672081/1‑70 (MQ=11) gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:236485/1‑70 (MQ=11) ccTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCa < 1:1479959/71‑1 (MQ=14) ccTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCa < 1:3840454/71‑1 (MQ=14) ccTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:1914965/1‑71 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1635542/63‑1 (MQ=35) ttATCCGCCCTACATGTGCAATTCCGTAACCTTGATAAAACGCGACAAGCg < 1:431/51‑1 (MQ=2) ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCg < 1:378486/51‑1 (MQ=11) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:5187757/1‑64 (MQ=39) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:5126278/1‑69 (MQ=255) aTCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACcgc > 1:2750132/1‑71 (MQ=14) aTCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:793690/60‑1 (MQ=11) aTCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:4944970/60‑1 (MQ=11) aTCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:2264733/60‑1 (MQ=11) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAcc < 1:502711/69‑1 (MQ=255) cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:1971297/41‑1 (MQ=9) cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:4477590/41‑1 (MQ=9) cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:4348493/41‑1 (MQ=9) cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:3316219/41‑1 (MQ=9) cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:401474/41‑1 (MQ=9) cccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaa > 1:364833/1‑57 (MQ=2) cccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc < 1:4959017/71‑1 (MQ=255) ccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAg < 1:2669289/41‑1 (MQ=9) tACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTc < 1:5638472/43‑1 (MQ=9) tACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGc < 1:502283/45‑1 (MQ=11) tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:3925711/1‑71 (MQ=255) tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:5323426/1‑71 (MQ=255) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:1671830/71‑1 (MQ=14) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:807848/71‑1 (MQ=14) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:3817910/71‑1 (MQ=14) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGc > 1:587354/1‑38 (MQ=40) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg > 1:3850333/1‑39 (MQ=40) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg > 1:5282379/1‑39 (MQ=40) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg > 1:3073076/1‑39 (MQ=40) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:4556734/71‑1 (MQ=255) aTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg > 1:4747650/1‑70 (MQ=255) gtgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGcgt > 1:2978124/1‑47 (MQ=11) gtgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGcgt > 1:2478874/1‑47 (MQ=11) cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg < 1:5055456/71‑1 (MQ=255) cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgc < 1:2173726/70‑1 (MQ=255) aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcgg < 1:3088174/71‑1 (MQ=255) ccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:988845/71‑1 (MQ=11) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:1764484/1‑58 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:26239/1‑58 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGt > 1:1779074/1‑70 (MQ=255) cGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg > 1:4811753/1‑70 (MQ=11) gtcggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:4506289/62‑1 (MQ=11) cggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCt > 1:3433141/2‑70 (MQ=14) ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa > 1:2150067/1‑71 (MQ=255) ggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCtt < 1:705783/70‑1 (MQ=14) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg < 1:2918214/47‑1 (MQ=255) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:969245/50‑1 (MQ=255) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:4773168/1‑71 (MQ=255) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:1463460/1‑71 (MQ=255) aTAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc > 1:2344458/1‑71 (MQ=18) tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATcc > 1:4978755/1‑71 (MQ=255) gACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc > 1:4877157/1‑70 (MQ=255) | TTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGC > minE/1614398‑1614535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |