| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,550 | C→T | 15.4% | intergenic (‑175/+42) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,550 | 0 | C | T | 15.4% | 155.3 / 17.1 | 65 | intergenic (‑175/+42) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base C (22/32); new base T (7/3); total (29/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
GCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACA > minE/1614481‑1614619 | gCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATcc > 1:2122357/1‑71 (MQ=255) aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:2698853/71‑1 (MQ=255) tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:4818242/1‑70 (MQ=18) tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:1423884/1‑70 (MQ=18) tCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGt < 1:1751831/71‑1 (MQ=21) cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTa < 1:2153166/70‑1 (MQ=18) cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1247214/70‑1 (MQ=255) cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1830344/70‑1 (MQ=255) cGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAg < 1:3673076/71‑1 (MQ=18) gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc > 1:3780197/1‑71 (MQ=255) cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt > 1:4676485/1‑71 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa > 1:3435211/1‑70 (MQ=18) tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa > 1:3225964/1‑70 (MQ=18) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGt > 1:5027997/1‑62 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa > 1:4208425/1‑70 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa > 1:2094292/1‑70 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaa < 1:38485/71‑1 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATa > 1:4353166/1‑70 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGAt < 1:2887924/69‑1 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:134099/71‑1 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATACCGTAGGCTGGATaa < 1:3377068/71‑1 (MQ=255) aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTaac > 1:2702758/1‑60 (MQ=17) aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaa < 1:2166123/71‑2 (MQ=17) gcgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg < 1:2684412/71‑1 (MQ=255) ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcgcga > 1:2501852/1‑70 (MQ=11) aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa > 1:4402637/1‑64 (MQ=39) aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa > 1:5423361/1‑64 (MQ=39) cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg > 1:694400/1‑65 (MQ=40) tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt < 1:3422101/58‑1 (MQ=35) ggCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt > 1:4950249/1‑57 (MQ=35) gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg < 1:3243313/71‑1 (MQ=39) gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:1443038/55‑1 (MQ=35) tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa > 1:1219639/1‑71 (MQ=39) tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa > 1:1135395/1‑71 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATcct < 1:2760224/71‑2 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg < 1:1312082/71‑1 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg < 1:1188101/71‑1 (MQ=39) cgTGACCGCCTTATCCGCCCTACATGTGCAATCCCg < 1:5157192/36‑1 (MQ=255) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:3404298/36‑1 (MQ=40) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:3818596/36‑1 (MQ=40) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg > 1:3390797/1‑71 (MQ=39) gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt > 1:5103668/1‑71 (MQ=38) cGCCTTATCCGCCCTACATTTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTa > 1:358416/1‑71 (MQ=255) cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTa < 1:4614628/71‑1 (MQ=40) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAt < 1:2353812/71‑1 (MQ=255) ccTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAtt > 1:777344/1‑71 (MQ=255) ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCa < 1:3886567/63‑1 (MQ=35) ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:1191221/65‑1 (MQ=39) ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:1659852/65‑1 (MQ=39) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg < 1:5028668/49‑1 (MQ=38) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:3872988/63‑1 (MQ=39) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2396444/63‑1 (MQ=39) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcgc > 1:372983/1‑71 (MQ=255) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGcgcc < 1:300626/71‑1 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt > 1:5454511/1‑47 (MQ=38) gCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCt < 1:1097927/71‑1 (MQ=255) gCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCt < 1:1043527/71‑1 (MQ=255) cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2940960/56‑1 (MQ=40) cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2772147/56‑1 (MQ=40) cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:1699595/56‑1 (MQ=40) tACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGc > 1:930632/1‑52 (MQ=35) tACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCg > 1:2958133/1‑70 (MQ=255) aCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCAt > 1:4692385/1‑46 (MQ=38) aCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTa > 1:384124/1‑71 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAttt > 1:4106416/1‑54 (MQ=255) tCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACa < 1:1823027/71‑1 (MQ=255) | GCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACA > minE/1614481‑1614619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |