| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,557 | G→C | 9.4% | intergenic (‑182/+35) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,557 | 0 | G | C | 9.4% | 184.6 / 7.7 | 64 | intergenic (‑182/+35) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (33/25); new base C (4/2); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTC > minE/1614488‑1614627 | gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc > 1:3780197/1‑71 (MQ=255) cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt > 1:4676485/1‑71 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa > 1:3225964/1‑70 (MQ=18) tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa > 1:3435211/1‑70 (MQ=18) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa > 1:2094292/1‑70 (MQ=255) tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa > 1:4208425/1‑70 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaa < 1:38485/71‑1 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATa > 1:4353166/1‑70 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGAt < 1:2887924/69‑1 (MQ=17) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:134099/71‑1 (MQ=255) cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATACCGTAGGCTGGATaa < 1:3377068/71‑1 (MQ=255) aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaa < 1:2166123/71‑2 (MQ=17) gcgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg < 1:2684412/71‑1 (MQ=255) ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcgcga > 1:2501852/1‑70 (MQ=11) aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa > 1:4402637/1‑64 (MQ=39) aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa > 1:5423361/1‑64 (MQ=39) cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg > 1:694400/1‑65 (MQ=40) tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt < 1:3422101/58‑1 (MQ=35) ggCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt > 1:4950249/1‑57 (MQ=35) gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg < 1:3243313/71‑1 (MQ=39) gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa < 1:1443038/55‑1 (MQ=35) tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa > 1:1219639/1‑71 (MQ=39) tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa > 1:1135395/1‑71 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATcct < 1:2760224/71‑2 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg < 1:1312082/71‑1 (MQ=39) gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg < 1:1188101/71‑1 (MQ=39) cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg > 1:3390797/1‑71 (MQ=39) gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt > 1:5103668/1‑71 (MQ=38) cGCCTTATCCGCCCTACATTTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTa > 1:358416/1‑71 (MQ=255) cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTa < 1:4614628/71‑1 (MQ=40) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAt < 1:2353812/71‑1 (MQ=255) ccTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAtt > 1:777344/1‑71 (MQ=255) ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCa < 1:3886567/63‑1 (MQ=35) ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:1191221/65‑1 (MQ=39) ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:1659852/65‑1 (MQ=39) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg < 1:5028668/49‑1 (MQ=38) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:3872988/63‑1 (MQ=39) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2396444/63‑1 (MQ=39) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcgc > 1:372983/1‑71 (MQ=255) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGcgcc < 1:300626/71‑1 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt > 1:5454511/1‑47 (MQ=38) gCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCt < 1:1043527/71‑1 (MQ=255) gCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCt < 1:1097927/71‑1 (MQ=255) cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:1699595/56‑1 (MQ=40) cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2772147/56‑1 (MQ=40) cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2940960/56‑1 (MQ=40) tACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGc > 1:930632/1‑52 (MQ=35) tACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCg > 1:2958133/1‑70 (MQ=255) aCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCAt > 1:4692385/1‑46 (MQ=38) aCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTa > 1:384124/1‑71 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAttt > 1:4106416/1‑54 (MQ=255) tCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACa < 1:1823027/71‑1 (MQ=255) ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt > 1:1072882/1‑71 (MQ=255) ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt > 1:2763398/1‑71 (MQ=255) ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt > 1:1153492/1‑71 (MQ=255) ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt > 1:1146633/1‑71 (MQ=255) gTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt > 1:1877804/1‑71 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCTGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg > 1:1671148/1‑71 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTg > 1:4987913/1‑52 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt > 1:3973118/1‑70 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg > 1:4648337/1‑71 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg > 1:5225213/1‑71 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg > 1:5400489/1‑71 (MQ=255) tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg > 1:758710/1‑71 (MQ=255) ggCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt < 1:2583690/37‑1 (MQ=255) ggCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCt > 1:3356269/1‑71 (MQ=255) gCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACa < 1:1625991/63‑1 (MQ=255) gCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTc < 1:4437047/71‑1 (MQ=255) gCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTc < 1:4766487/71‑1 (MQ=255) | GCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTC > minE/1614488‑1614627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |