| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,629,895 | C→A | 12.5% | V633V (GTG→GTT) | aegA ← | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,629,895 | 0 | C | A | 12.5% | 212.3 / 9.3 | 70 | V633V (GTG→GTT) | aegA | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (30/31); new base A (4/5); total (34/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACTGATTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAC > minE/1629826‑1629964 | gACTGATTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAacca > 1:2190950/1‑71 (MQ=255) cTGATTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAaccacc < 1:889878/71‑1 (MQ=255) cTGATTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAaccacc < 1:4151787/71‑1 (MQ=255) cTGATTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAaccacc < 1:5292382/71‑1 (MQ=255) aTTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAg < 1:3077788/70‑1 (MQ=255) aTTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGa < 1:424007/71‑1 (MQ=255) ttttACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAaccac > 1:329004/1‑66 (MQ=255) ccccAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGa < 1:5186335/64‑1 (MQ=255) ccccAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGa < 1:2443061/64‑1 (MQ=255) ccccAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGcac < 1:2857216/71‑1 (MQ=255) ccagccagTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGa > 1:3663022/1‑62 (MQ=255) ccagccagTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGcacca > 1:1959509/1‑71 (MQ=255) ccagccagTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGcacca > 1:2145963/1‑71 (MQ=255) ccagccagTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGcacca > 1:3935993/1‑71 (MQ=255) agTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAAccacca > 1:320343/1‑54 (MQ=255) aatgatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGa < 1:2454803/52‑1 (MQ=255) aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGAt > 1:4407690/1‑53 (MQ=255) aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACgg > 1:3839548/1‑68 (MQ=255) aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACgg > 1:4137203/1‑68 (MQ=255) aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt < 1:5431478/71‑1 (MQ=255) aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt > 1:2434561/1‑71 (MQ=255) aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt < 1:1828145/71‑1 (MQ=255) aataatACCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt > 1:3448/1‑71 (MQ=255) ataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt < 1:5437777/70‑1 (MQ=255) ataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTc < 1:2688501/71‑1 (MQ=255) aatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAg > 1:1523683/1‑48 (MQ=255) aatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAg > 1:1565966/1‑48 (MQ=255) aatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcac > 1:1581383/1‑71 (MQ=255) tCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcacca > 1:288794/1‑71 (MQ=255) cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATcc < 1:3379958/48‑1 (MQ=255) cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac > 1:2983440/1‑70 (MQ=255) cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac > 1:2276367/1‑70 (MQ=255) cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCaccacc < 1:4186157/71‑1 (MQ=255) cgcgccgccTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGc < 1:3800687/68‑1 (MQ=255) tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac > 1:2781339/1‑65 (MQ=255) tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac > 1:885890/1‑65 (MQ=255) tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCg < 1:2499490/70‑1 (MQ=255) tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCg < 1:3924902/70‑1 (MQ=255) ccGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGca > 1:1818578/1‑67 (MQ=255) cATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAtt < 1:3212266/71‑1 (MQ=255) aTGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAttt < 1:2672164/71‑1 (MQ=255) aCGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc < 1:2910727/70‑1 (MQ=255) tCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATca > 1:476990/1‑49 (MQ=255) ccTTCTGCCATTGCGGTAACCACCAGATCCGTACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGAtt > 1:3917505/1‑71 (MQ=255) ccTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAtt < 1:2607303/63‑1 (MQ=255) ccTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGAtt > 1:5446307/1‑71 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCgg > 1:4583245/1‑67 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg < 1:5380573/71‑1 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg < 1:5371234/71‑1 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg > 1:2885266/1‑71 (MQ=255) tCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggt > 1:2667186/1‑71 (MQ=255) tCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggt > 1:2673643/1‑71 (MQ=255) cTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGa > 1:868906/1‑65 (MQ=255) cTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGa > 1:2007297/1‑65 (MQ=255) ccATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggtg < 1:4466455/68‑1 (MQ=255) cATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCt > 1:3575533/1‑71 (MQ=255) aTTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc < 1:4542820/57‑1 (MQ=255) aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa < 1:1409255/50‑1 (MQ=255) aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa < 1:1465982/50‑1 (MQ=255) aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActtt > 1:5448334/1‑56 (MQ=255) ttGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCg < 1:4321572/57‑1 (MQ=255) ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt < 1:1510369/56‑2 (MQ=255) ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt < 1:2644609/56‑2 (MQ=255) tGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt > 1:4026992/1‑54 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGTATTGGTGGTCTCGt > 1:787550/1‑70 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt < 1:4658968/70‑1 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt < 1:5056635/70‑1 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt > 1:5336268/1‑70 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTcaccac < 1:3927757/37‑1 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTcaccac < 1:3093120/37‑1 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTcaccac < 1:1387995/37‑1 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGa < 1:5371855/46‑1 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGa < 1:206108/46‑1 (MQ=255) tAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACgg < 1:5342025/71‑1 (MQ=255) aaCCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc > 1:3175616/1‑49 (MQ=255) aaCCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc > 1:304173/1‑49 (MQ=255) accaccAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa > 1:1746014/1‑71 (MQ=255) accaccAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa > 1:5180842/1‑71 (MQ=255) ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc < 1:5418865/71‑1 (MQ=255) ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc < 1:1456046/71‑1 (MQ=255) ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc < 1:81339/71‑1 (MQ=255) | GACTGATTTTACCCCCAGCCAGTCAATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAC > minE/1629826‑1629964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |