Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,629,919 G→A 11.7% D625D (GAC→GAT aegA ← fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,629,9190GA11.7% 224.7 / 14.8 85D625D (GAC→GATaegAfused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (38/37);  new base A (4/6);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGA  >  minE/1629850‑1629986
                                                                     |                                                                   
aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt                                                                    >  1:2434561/1‑71 (MQ=255)
aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt                                                                    <  1:5431478/71‑1 (MQ=255)
aataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt                                                                    <  1:1828145/71‑1 (MQ=255)
aataatACCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt                                                                    >  1:3448/1‑71 (MQ=255)
 ataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGt                                                                    <  1:5437777/70‑1 (MQ=255)
 ataatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTc                                                                   <  1:2688501/71‑1 (MQ=255)
   aatCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcac                                                                 >  1:1581383/1‑71 (MQ=255)
     tCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcacca                                                               >  1:288794/1‑71 (MQ=255)
       cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              >  1:2983440/1‑70 (MQ=255)
       cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              >  1:2276367/1‑70 (MQ=255)
       cccTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCaccacc                                                             <  1:4186157/71‑1 (MQ=255)
          cgcgccgccTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGc                                                          <  1:3800687/68‑1 (MQ=255)
            tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              >  1:885890/1‑65 (MQ=255)
            tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              >  1:2781339/1‑65 (MQ=255)
            tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCg                                                         <  1:3924902/70‑1 (MQ=255)
            tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCg                                                         <  1:2499490/70‑1 (MQ=255)
              ccGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGca                                                         >  1:1818578/1‑67 (MQ=255)
                 cATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAtt                                                   <  1:3212266/71‑1 (MQ=255)
                  aTGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAttt                                                  <  1:2672164/71‑1 (MQ=255)
                     aCGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc                                                <  1:2910727/70‑1 (MQ=255)
                        tCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATca                                                                  >  1:476990/1‑49 (MQ=255)
                         ccTTCTGCCATTGCGGTAACCACCAGATCCGTACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGAtt                                           >  1:3917505/1‑71 (MQ=255)
                         ccTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAtt                                                   <  1:2607303/63‑1 (MQ=255)
                         ccTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGAtt                                           >  1:5446307/1‑71 (MQ=255)
                          cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCgg                                              >  1:4583245/1‑67 (MQ=255)
                          cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg                                          <  1:5380573/71‑1 (MQ=255)
                          cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg                                          <  1:5371234/71‑1 (MQ=255)
                          cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg                                          >  1:2885266/1‑71 (MQ=255)
                            tCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggt                                        >  1:2667186/1‑71 (MQ=255)
                            tCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggt                                        >  1:2673643/1‑71 (MQ=255)
                             cTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGa                                             >  1:868906/1‑65 (MQ=255)
                             cTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGa                                             >  1:2007297/1‑65 (MQ=255)
                                ccATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggtg                                       <  1:4466455/68‑1 (MQ=255)
                                 cATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCt                                   >  1:3575533/1‑71 (MQ=255)
                                  aTTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc                                                <  1:4542820/57‑1 (MQ=255)
                                  aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa                                                       <  1:1465982/50‑1 (MQ=255)
                                  aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa                                                       <  1:1409255/50‑1 (MQ=255)
                                  aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActtt                                                 >  1:5448334/1‑56 (MQ=255)
                                   ttGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCg                                               <  1:4321572/57‑1 (MQ=255)
                                   ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt                                                <  1:2644609/56‑2 (MQ=255)
                                   ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt                                                <  1:1510369/56‑2 (MQ=255)
                                    tGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt                                                >  1:4026992/1‑54 (MQ=255)
                                     gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGTATTGGTGGTCTCGt                                >  1:787550/1‑70 (MQ=255)
                                     gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt                                <  1:5056635/70‑1 (MQ=255)
                                     gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt                                <  1:4658968/70‑1 (MQ=255)
                                     gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt                                >  1:5336268/1‑70 (MQ=255)
                                        gTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              <  1:3093120/37‑1 (MQ=255)
                                        gTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              <  1:3927757/37‑1 (MQ=255)
                                        gTAACCACCAGATCCGCACCACGCACGGCGTcaccac                                                              <  1:1387995/37‑1 (MQ=255)
                                        gTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGa                                                     <  1:206108/46‑1 (MQ=255)
                                        gTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGa                                                     <  1:5371855/46‑1 (MQ=255)
                                         tAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACgg                           <  1:5342025/71‑1 (MQ=255)
                                          aaCCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc                                                >  1:3175616/1‑49 (MQ=255)
                                          aaCCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc                                                >  1:304173/1‑49 (MQ=255)
                                           accaccAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa                         >  1:5180842/1‑71 (MQ=255)
                                           accaccAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa                         >  1:1746014/1‑71 (MQ=255)
                                            ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc                        <  1:1456046/71‑1 (MQ=255)
                                            ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc                        <  1:81339/71‑1 (MQ=255)
                                            ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc                        <  1:5418865/71‑1 (MQ=255)
                                              accaGATCCGCACCACGCACGGCGTCACCACCAGCGaa                                                       >  1:1399524/1‑38 (MQ=255)
                                              accaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTg                      <  1:4857642/71‑1 (MQ=255)
                                              accaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTg                      >  1:1966256/1‑71 (MQ=255)
                                               ccaGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt                                                <  1:2178597/44‑2 (MQ=12)
                                               ccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa                         >  1:2650219/1‑67 (MQ=255)
                                               ccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTgg                     >  1:436426/1‑71 (MQ=255)
                                                caGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGc                    >  1:5479932/1‑71 (MQ=255)
                                                 aGATCCGCACCACGCACGGCGTCACCACCAGCGaa                                                       <  1:1215272/35‑1 (MQ=255)
                                                 aGATCCGCACCACGCACGGCGTCACCACCAGCGaa                                                       <  1:4185449/35‑1 (MQ=255)
                                                 aGATCCGCACCACGCACGGCGTCACCACCAGCGaa                                                       <  1:2228132/35‑1 (MQ=255)
                                                 aGATCCGCACCACGCACGGCGTCACCACCAGCGaa                                                       <  1:1684884/35‑1 (MQ=255)
                                                  gATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCtt                  <  1:974146/71‑1 (MQ=255)
                                                   aTCCGCACCACGGACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTaa                              >  1:4447349/1‑58 (MQ=255)
                                                   aTCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTaa                              >  1:83229/1‑58 (MQ=255)
                                                      cGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg                                          <  1:160355/43‑1 (MQ=255)
                                                            acgcacgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCcg        >  1:1056952/1‑71 (MQ=255)
                                                             cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg                                         >  1:4517921/1‑37 (MQ=255)
                                                             cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg                                         >  1:2217188/1‑37 (MQ=255)
                                                             cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg                                         >  1:4959158/1‑37 (MQ=255)
                                                             cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg                                         >  1:675440/1‑37 (MQ=255)
                                                             cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtg                                          >  1:3466304/1‑36 (MQ=255)
                                                             cgcacgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCcgc       <  1:1726528/71‑1 (MQ=255)
                                                               cacgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCga     >  1:4573666/1‑71 (MQ=255)
                                                                acgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa                         >  1:148331/1‑50 (MQ=255)
                                                                acgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCgat    <  1:1669496/71‑1 (MQ=255)
                                                                  ggCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTGCACATCCGCgatga  <  1:1905701/71‑1 (MQ=255)
                                                                     |                                                                   
AATAATCCCCTGTGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGA  >  minE/1629850‑1629986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: