| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,629,931 | G→A | 6.5% | F621F (TTC→TTT) | aegA ← | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,629,931 | 0 | G | A | 6.5% | 228.1 / 3.9 | 76 | F621F (TTC→TTT) | aegA | fused predicted FeS binding subunit and predicted NAD/FAD‑binding subunit of oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (36/35); new base A (2/3); total (38/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGATGCGGCCCCATTT > minE/1629862‑1629999 | tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCg < 1:3924902/70‑1 (MQ=255)tgCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCg < 1:2499490/70‑1 (MQ=255) cATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAtt < 1:3212266/71‑1 (MQ=255) aTGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAttt < 1:2672164/71‑1 (MQ=255) aCGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc < 1:2910727/70‑1 (MQ=255) ccTTCTGCCATTGCGGTAACCACCAGATCCGTACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGAtt > 1:3917505/1‑71 (MQ=255) ccTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGAtt < 1:2607303/63‑1 (MQ=255) ccTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGAtt > 1:5446307/1‑71 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCgg > 1:4583245/1‑67 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg > 1:2885266/1‑71 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg < 1:5371234/71‑1 (MQ=255) cTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg < 1:5380573/71‑1 (MQ=255) tCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggt > 1:2673643/1‑71 (MQ=255) tCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggt > 1:2667186/1‑71 (MQ=255) cTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGa > 1:868906/1‑65 (MQ=255) cTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGa > 1:2007297/1‑65 (MQ=255) ccATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtggtg < 1:4466455/68‑1 (MQ=255) cATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCt > 1:3575533/1‑71 (MQ=255) aTTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc < 1:4542820/57‑1 (MQ=255) aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa < 1:1409255/50‑1 (MQ=255) aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa < 1:1465982/50‑1 (MQ=255) aTTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActtt > 1:5448334/1‑56 (MQ=255) ttGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCg < 1:4321572/57‑1 (MQ=255) ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt < 1:1510369/56‑2 (MQ=255) ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt < 1:2644609/56‑2 (MQ=255) tGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt > 1:4026992/1‑54 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGTATTGGTGGTCTCGt > 1:787550/1‑70 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt < 1:5056635/70‑1 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt < 1:4658968/70‑1 (MQ=255) gCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGt > 1:5336268/1‑70 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGa < 1:5371855/46‑1 (MQ=255) gTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGa < 1:206108/46‑1 (MQ=255) tAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACgg < 1:5342025/71‑1 (MQ=255) aaCCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc > 1:304173/1‑49 (MQ=255) aaCCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTc > 1:3175616/1‑49 (MQ=255) accaccAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa > 1:1746014/1‑71 (MQ=255) accaccAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa > 1:5180842/1‑71 (MQ=255) ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc < 1:5418865/71‑1 (MQ=255) ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc < 1:1456046/71‑1 (MQ=255) ccaccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTAc < 1:81339/71‑1 (MQ=255) accaGATCCGCACCACGCACGGCGTCACCACCAGCGaa > 1:1399524/1‑38 (MQ=255) accaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTg < 1:4857642/71‑1 (MQ=255) accaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTg > 1:1966256/1‑71 (MQ=255) ccaGATCCGCGCCATGAACTGCATCACCACCAGCAAAGActttt < 1:2178597/44‑2 (MQ=12) ccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa > 1:2650219/1‑67 (MQ=255) ccaGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTgg > 1:436426/1‑71 (MQ=255) caGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGc > 1:5479932/1‑71 (MQ=255) aGATCCGCACCACGCACGGCGTCACCACCAGCGaa < 1:2228132/35‑1 (MQ=255) aGATCCGCACCACGCACGGCGTCACCACCAGCGaa < 1:4185449/35‑1 (MQ=255) aGATCCGCACCACGCACGGCGTCACCACCAGCGaa < 1:1684884/35‑1 (MQ=255) aGATCCGCACCACGCACGGCGTCACCACCAGCGaa < 1:1215272/35‑1 (MQ=255) gATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCtt < 1:974146/71‑1 (MQ=255) aTCCGCACCACGGACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTaa > 1:4447349/1‑58 (MQ=255) aTCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTaa > 1:83229/1‑58 (MQ=255) cGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATtg < 1:160355/43‑1 (MQ=255) acgcacgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCcg > 1:1056952/1‑71 (MQ=255) cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg > 1:4959158/1‑37 (MQ=255) cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg > 1:675440/1‑37 (MQ=255) cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg > 1:4517921/1‑37 (MQ=255) cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtgg > 1:2217188/1‑37 (MQ=255) cgcacgGCGTCACCACCAGCGAAGATTTTCGGATtg > 1:3466304/1‑36 (MQ=255) cgcacgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCcgc < 1:1726528/71‑1 (MQ=255) cacgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCga > 1:4573666/1‑71 (MQ=255) acgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTa > 1:148331/1‑50 (MQ=255) acgGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCgat < 1:1669496/71‑1 (MQ=255) ggCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTGCACATCCGCgatga < 1:1905701/71‑1 (MQ=255) tCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTg < 1:3298822/47‑1 (MQ=255) tCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCcgc < 1:5606620/62‑1 (MQ=255) tCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCcgc < 1:2590588/62‑1 (MQ=255) accaccAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCgatga > 1:677265/1‑65 (MQ=255) accaccAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCgatga > 1:3527494/1‑65 (MQ=255) accaccAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCgatga > 1:2572632/1‑65 (MQ=255) caccaGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGATGCGGccc < 1:4887272/71‑1 (MQ=255) accaGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCgatga > 1:5328820/1‑62 (MQ=255) accaGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGATGCGGccc > 1:735110/1‑70 (MQ=255) aGCGAAGTTTTTCGGATTGGTGGTCTGGTAACGGTTCTGGCTTTCcac < 1:774112/48‑1 (MQ=38) aGCGAAGATTTTCGGATTGGTTGTCTGGTAACGGTACTGGCTTTCcac < 1:108180/48‑1 (MQ=255) aGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCcac < 1:2891137/48‑1 (MQ=255) aGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGATGCGGCCCCAtt > 1:5023026/1‑71 (MQ=255) gCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGATGCGGCCCCAttt < 1:3222892/71‑1 (MQ=255) gAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTc < 1:4975070/42‑1 (MQ=255) gAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTc < 1:4789348/42‑1 (MQ=255) gAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTc < 1:4562582/42‑1 (MQ=255) gAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTc < 1:3770441/42‑1 (MQ=255) gAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCca > 1:2185656/1‑44 (MQ=255) | TGCCGCATGACGTCCTTCTGCCATTGCGGTAACCACCAGATCCGCACCACGCACGGCGTCACCACCAGCGAAGATTTTCGGATTGGTGGTCTGGTAACGGTACTGGCTTTCCACATCCGCGATGATGCGGCCCCATTT > minE/1629862‑1629999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |