| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,639,287 | A→G | 8.7% | intergenic (+57/+53) | ypfN → / ← ypfH | hypothetical protein/predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,639,287 | 0 | A | G | 8.7% | 256.1 / 11.4 | 94 | intergenic (+57/+53) | ypfN/ypfH | hypothetical protein/predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (42/43); new base G (2/6); total (45/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
CCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAA > minE/1639219‑1639353 | ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc > 1:4502322/1‑71 (MQ=255)ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:3442558/71‑1 (MQ=255)ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:3704294/71‑1 (MQ=255) cGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:773098/70‑1 (MQ=255) gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGa > 1:511664/1‑71 (MQ=255) gaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGc < 1:2470358/71‑1 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCt > 1:841144/1‑70 (MQ=255) aaGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGAt > 1:4334209/1‑71 (MQ=255) gCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGc < 1:2063037/71‑1 (MQ=255) tGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGa > 1:217416/1‑71 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGa < 1:1472496/69‑1 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACg < 1:170100/71‑1 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACg < 1:176796/71‑1 (MQ=255) gTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGc < 1:754492/71‑1 (MQ=255) tGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCt < 1:3028274/71‑1 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat > 1:1694301‑M1/4‑46 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat > 1:2786583‑M1/4‑46 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat < 1:2626015‑M1/68‑26 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat < 1:4143815‑M1/68‑26 (MQ=255) actcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat < 1:4576253‑M1/68‑26 (MQ=255) actcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgcta > 1:800526‑M1/4‑46 (MQ=255) actcgtaGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttat < 1:3539727‑M1/68‑26 (MQ=255) actcgtaGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttat < 1:3142512‑M1/68‑26 (MQ=255) ctcgtAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat > 1:766880‑M1/3‑45 (MQ=255) ctcgtAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatc < 1:3266569‑M1/69‑27 (MQ=255) tcgTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatg > 1:3737166‑M1/2‑44 (MQ=255) tcgTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaa > 1:417648‑M1/2‑44 (MQ=255) cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:3560763‑M1/1‑43 (MQ=255) cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAca < 1:2398275‑M1/45‑3 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcagg > 1:3620561‑M1/1‑42 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcag < 1:4664754‑M1/70‑29 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcag < 1:2527874‑M1/70‑29 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatca < 1:2292632‑M1/69‑28 (MQ=255) gTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatca < 1:2061615‑M1/69‑28 (MQ=255) aGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTa > 1:3526884/1‑71 (MQ=255) aGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttat < 1:2228200‑M1/65‑26 (MQ=255) ggCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTAt < 1:3121612/71‑1 (MQ=255) ggCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTAt < 1:1179540/71‑1 (MQ=255) gCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatcaggccta < 1:2665404‑M1/71‑34 (MQ=255) cGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatcaggccta > 1:3745869‑M1/1‑36 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggccta > 1:1051323‑M1/1‑35 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatca > 1:2540654‑M1/1‑35 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatca > 1:1768925‑M1/1‑35 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctacaga < 1:415688‑M1/71‑38 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctacaga < 1:3744905‑M1/71‑38 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAca < 1:2947988‑M1/36‑3 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAca < 1:860661‑M1/36‑3 (MQ=255) gATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:2174536/36‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcct < 1:1256639‑M1/42‑10 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcct < 1:3769719‑M1/42‑10 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcct < 1:1726267‑M1/42‑10 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGcc < 1:4354912/71‑1 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGcc < 1:4210460/71‑1 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGcc < 1:1902689/71‑1 (MQ=255) tAAGGCGTTTACGCCGCATCCGGCAACAGTG‑CCGACTGCCTGATGCGACGCTCGCGCGTCTTATCAGGccg > 1:718344/1‑70 (MQ=1) aaGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctga > 1:60934‑M1/1‑31 (MQ=255) gCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTa < 1:4425751/71‑1 (MQ=255) cGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTaa > 1:5151097/1‑71 (MQ=255) gTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctacaga < 1:651551‑M1/63‑38 (MQ=255) gTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCt < 1:4731550/35‑1 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTAt < 1:2366829/57‑1 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGc < 1:3638156/62‑1 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc > 1:1261933/1‑71 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc > 1:3411478/1‑71 (MQ=255) tAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGc < 1:841510/61‑1 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCa > 1:2613281/1‑57 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:4503588/1‑71 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:931845/1‑71 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:1180973/1‑71 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:3506308/1‑71 (MQ=255) ccgcATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTa > 1:3891078/1‑62 (MQ=255) cgcATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt > 1:5414319/1‑71 (MQ=255) gcATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctaca > 1:2911165‑M1/1‑17 (MQ=255) gcATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTa < 1:5360181/48‑1 (MQ=255) cATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGt > 1:2983973/1‑58 (MQ=255) ccGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:4036882‑M1/1‑13 (MQ=255) ccGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:2782819‑M1/1‑13 (MQ=255) ccGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:3345929‑M1/1‑13 (MQ=255) ccGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:4226855‑M1/1‑13 (MQ=255) ccGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:3830605‑M1/1‑13 (MQ=255) ccGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:3260030‑M1/1‑13 (MQ=255) ccGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTa > 1:5385728/1‑68 (MQ=255) cGGCAGTGGTGGcaaatgcctgatgcgctacgcttat > 1:1302286‑M1/1‑12 (MQ=255) cGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcat < 1:4598506/70‑1 (MQ=255) cGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcat > 1:2368769/1‑70 (MQ=255) ggCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcatca < 1:3014156/71‑1 (MQ=255) cAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctaca < 1:62743‑M1/44‑36 (MQ=255) cAATGGTGAcaaatgcctgatgcgctacgcttatca < 1:3056331‑M1/36‑28 (MQ=255) cAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTa > 1:2206842/1‑40 (MQ=255) cAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTGTACATCAtt > 1:3445305/1‑71 (MQ=255) aTGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTc < 1:1408944/71‑1 (MQ=255) tGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCa < 1:2961427/71‑1 (MQ=255) gagAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCaa > 1:661652/3‑70 (MQ=255) gTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCa > 1:4591110/1‑38 (MQ=255) | CCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAA > minE/1639219‑1639353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |