| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,716,832 | A→C | 18.3% | intergenic (+90/+108) | yphH → / ← glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,716,832 | 0 | A | C | 18.3% | 131.2 / 43.8 | 98 | intergenic (+90/+108) | yphH/glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (49/31); new base C (8/10); total (57/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
GCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT > minE/1716763‑1716902 | gCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTacac > 1:79268/1‑71 (MQ=255) cccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACg > 1:725093/1‑68 (MQ=255) cccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACg > 1:5406542/1‑68 (MQ=255) cccGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGga < 1:1278834/70‑1 (MQ=255) gaaggaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGga > 1:4538483/1‑67 (MQ=255) gaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGc < 1:830322/69‑1 (MQ=255) gaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCaa < 1:1382750/71‑1 (MQ=255) gaagCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCaa < 1:4219521/71‑1 (MQ=255) tgatgaTCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATAtt > 1:2158306/1‑70 (MQ=255) tgatgaTCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATAtt > 1:5167321/1‑70 (MQ=255) atCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGc < 1:4501282/57‑1 (MQ=255) atCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGc < 1:1722259/57‑1 (MQ=255) tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGa > 1:5353944/1‑66 (MQ=255) tCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTg > 1:2183963/1‑71 (MQ=255) cATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt > 1:2953982/1‑69 (MQ=255) cATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt > 1:2209273/1‑69 (MQ=255) cATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAttt > 1:1408933/1‑69 (MQ=255) cATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTg > 1:4178114/1‑70 (MQ=255) tAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAatatat < 1:1149807/60‑1 (MQ=255) aaaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:5673330/1‑48 (MQ=255) aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACgg > 1:4679555/1‑44 (MQ=255) aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTg > 1:2996130/1‑60 (MQ=255) aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTg > 1:4525507/1‑60 (MQ=255) aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAg > 1:621064/1‑71 (MQ=255) caaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg < 1:1355129/70‑1 (MQ=11) caaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg < 1:1070969/70‑1 (MQ=11) caaaTTGCCTCATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg < 1:4129553/70‑1 (MQ=11) aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAatatat > 1:4963288/1‑53 (MQ=255) agcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTg > 1:4318637‑M2/20‑60 (MQ=255) agcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:345139‑M2/51‑1 (MQ=255) agcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:334856‑M2/51‑1 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:378560/1‑41 (MQ=40) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:3653164/1‑41 (MQ=40) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:4091429/1‑41 (MQ=40) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:3717694/1‑41 (MQ=40) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:1479918/1‑41 (MQ=40) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGgaga > 1:1042618/1‑41 (MQ=40) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg > 1:1029988/1‑70 (MQ=11) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGt < 1:1405716/71‑1 (MQ=11) gcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg > 1:100149‑M2/19‑69 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt < 1:1965428/70‑1 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGt < 1:1123517/70‑1 (MQ=11) aTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAg < 1:280107/71‑1 (MQ=255) ttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAata < 1:829910‑M2/30‑1 (MQ=255) ttGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAg > 1:4964856/1‑70 (MQ=11) gcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAatata > 1:24882‑M2/15‑46 (MQ=255) gcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAatata > 1:3719605‑M2/15‑46 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAggac < 1:3626879/70‑3 (MQ=11) ccTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga < 1:5333843/69‑2 (MQ=11) tGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:87508/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAAt < 1:3524256/48‑1 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCa > 1:4517591‑M2/10‑36 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATa < 1:5482606‑M2/62‑1 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATa < 1:276209‑M2/62‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:5241154/1‑61 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:3472443/1‑61 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:3465622/1‑61 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa < 1:64705/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:1271959/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:5506823/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt < 1:1233144/70‑1 (MQ=11) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt < 1:4987178/70‑1 (MQ=11) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATa < 1:2054670/71‑1 (MQ=11) tgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:303370‑M2/51‑1 (MQ=255) tgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:5484282‑M2/51‑1 (MQ=255) cgcgatGCTTATCAGGCCTACCAGAAGATTGCAata > 1:4175209‑M2/7‑36 (MQ=255) cgcTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg > 1:1208984/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:3703517/70‑1 (MQ=11) cgcTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:4010410/70‑1 (MQ=11) cgatGCTTATCAGGCCTACA‑GAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGt < 1:2714889‑M2/67‑1 (MQ=255) atGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGtttt < 1:5031911‑M2/50‑1 (MQ=255) atGCTTATCAGGCCTACCAGAAGATAGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGtt < 1:4203362‑M2/69‑1 (MQ=255) aCGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:1349458/1‑62 (MQ=255) aCGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:4984271/1‑62 (MQ=255) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:4816340/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:5616760/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:5119812/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:293058/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgct > 1:4413369/1‑69 (MQ=2) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCaaa > 1:2113532/1‑46 (MQ=255) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCaaa > 1:1220582/1‑46 (MQ=255) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt < 1:5436240/70‑1 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:2513460/1‑70 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:4071410/1‑70 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCAc > 1:3248722/1‑71 (MQ=255) cTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATATGCGATGTTTTGTAGGACTGATAAGGCGCTcg > 1:5395870/1‑69 (MQ=2) tATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:109984/1‑52 (MQ=2) tCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTa < 1:4110570/48‑1 (MQ=11) aGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAgg > 1:3164094/1‑48 (MQ=11) aGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAgg > 1:3956611/1‑48 (MQ=11) ccTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:5218794/51‑1 (MQ=255) tACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:1792834/50‑1 (MQ=255) tACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:529082/50‑1 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:5162570/1‑38 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa > 1:1550923/1‑49 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:1556834/1‑50 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:4125468/1‑50 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5050166/69‑1 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa > 1:5061555/1‑48 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt > 1:5566762/1‑71 (MQ=255) | GCTTCCCGAAGGAAGCCTTGATGATCATAAACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT > minE/1716763‑1716902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |