| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,859 | G→A | 26.7% | T1005I (ACC→ATC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,859 | 0 | G | A | 26.7% | 112.4 / 42.9 | 71 | T1005I (ACC→ATC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (29/23); new base A (8/11); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
CACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAA > minE/309792‑309929 | cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc > 1:4126667/1‑71 (MQ=255) acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc > 1:5554540/1‑71 (MQ=255) aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:5162098/1‑70 (MQ=255) aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca > 1:849491/1‑68 (MQ=12) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:847248/67‑1 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:625993/67‑1 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc < 1:182003/67‑1 (MQ=255) gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag > 1:2923753/1‑69 (MQ=255) acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg > 1:3298615/3‑71 (MQ=255) acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg > 1:4712527/3‑71 (MQ=255) tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:5670355/1‑65 (MQ=255) tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa > 1:2475731/1‑65 (MQ=255) aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:5275557/71‑1 (MQ=255) cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca > 1:5372972/1‑60 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg > 1:309971/1‑71 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg < 1:2365160/71‑1 (MQ=255) gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc < 1:2910880/69‑1 (MQ=255) gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc < 1:2736173/69‑1 (MQ=255) gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc < 1:2729606/71‑1 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc > 1:2623255/1‑71 (MQ=255) cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg > 1:2352911/1‑71 (MQ=255) cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt > 1:487488/1‑64 (MQ=255) cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt > 1:1336105/1‑64 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc < 1:4858816/71‑1 (MQ=255) gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc < 1:1760126/71‑1 (MQ=12) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:4603109/62‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:1588986/62‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:2606199/62‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1517037/71‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1306413/71‑1 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa < 1:448472/71‑1 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc > 1:3420077/1‑71 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc > 1:3216696/1‑71 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:91297/71‑1 (MQ=255) aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:2801117/70‑1 (MQ=255) aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg < 1:4018313/63‑1 (MQ=255) aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg < 1:4069408/63‑1 (MQ=255) gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:4999943/1‑70 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:1582566/1‑71 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:5516986/1‑70 (MQ=255) ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa > 1:2665121/1‑71 (MQ=255) gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt > 1:1012766/1‑71 (MQ=255) ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTCTGCGCGCCGGAACCAGCACCAGTACt > 1:4175240/1‑70 (MQ=255) cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt < 1:468344/70‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:4097617/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:5169313/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:4352798/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa < 1:2850347/70‑1 (MQ=255) tCACCATCCCGCCCATTACACCGGTACCTACTGCg < 1:719907/35‑1 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg < 1:2622107/71‑1 (MQ=255) cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc > 1:1679130/1‑66 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGa < 1:1986799/43‑1 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTg > 1:2983748/1‑59 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg > 1:2489622/1‑70 (MQ=255) ccGCCCATTACACCGGTACCTACTGCGTTCTgcgc > 1:4285740/1‑35 (MQ=255) ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa > 1:2221300/1‑70 (MQ=255) cGCCCATTACACCGGTACCTACTGCGTTCTGCGGGCCGGAACCAGCAcc < 1:1454465/49‑1 (MQ=255) gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:4182180/1‑44 (MQ=255) gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:1062951/1‑44 (MQ=255) ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACg > 1:3367986/1‑71 (MQ=255) aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc < 1:5062096/71‑1 (MQ=255) aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc < 1:4369787/71‑1 (MQ=255) ttACCCCGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGcc > 1:2923481/1‑70 (MQ=255) tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc > 1:2110669/1‑69 (MQ=255) tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc > 1:2464934/1‑69 (MQ=255) acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAg > 1:2989727/1‑71 (MQ=255) ccccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg < 1:4620256/68‑1 (MQ=255) ccccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg < 1:3655006/68‑1 (MQ=255) cacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa > 1:3283800/1‑63 (MQ=255) cGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa < 1:5235571/71‑1 (MQ=255) cGGTACCTACTGCGTTCTCCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGAt > 1:1016418/1‑70 (MQ=255) ggTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaa < 1:4032596/71‑1 (MQ=255) gTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa < 1:5169148/71‑1 (MQ=255) | CACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAA > minE/309792‑309929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |