| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,801,465 | C→G | 6.4% | intergenic (+176/‑16) | proX → / → ygaX | glycine betaine transporter subunit/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,801,465 | 0 | C | G | 6.4% | 329.8 / 8.5 | 109 | intergenic (+176/‑16) | proX/ygaX | glycine betaine transporter subunit/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (35/67); new base G (5/2); total (40/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
TTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCT > minE/1801395‑1801531 | tttCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCAcc > 1:1550595/1‑71 (MQ=255) tCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCt > 1:4456631/1‑70 (MQ=255) caacaaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtga > 1:884399/1‑71 (MQ=255) caacaaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtg < 1:2628795/70‑1 (MQ=255) aacaaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgat < 1:1210248/71‑1 (MQ=255) aacaaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgat < 1:3318338/71‑1 (MQ=255) aacaaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgat > 1:3232989/1‑71 (MQ=255) aacaaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtga < 1:4843374/70‑1 (MQ=255) caaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgatga < 1:5088877/71‑1 (MQ=255) caaTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCtgatga < 1:2109508/71‑1 (MQ=255) tatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaa > 1:4387788/1‑70 (MQ=255) tctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaa < 1:3641821/68‑1 (MQ=255) ttCATCTCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaataa > 1:734854/1‑71 (MQ=255) tCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACaa > 1:5660349/1‑64 (MQ=255) tCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat > 1:1803679/1‑71 (MQ=255) tCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaat < 1:3834364/68‑1 (MQ=255) cATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat < 1:4385582/70‑1 (MQ=255) cATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat < 1:3511544/70‑1 (MQ=255) cATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat < 1:4629384/70‑1 (MQ=255) cATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaa < 1:3504069/71‑1 (MQ=255) atatTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTaaa > 1:494387/1‑71 (MQ=255) ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAAcc > 1:2800693/1‑70 (MQ=255) ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCt < 1:2424832/71‑1 (MQ=255) ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCt < 1:3045679/71‑1 (MQ=255) ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGAAGAACAAAAATAATGa > 1:3505598/1‑63 (MQ=255) atcaACATCCGCTATTATTGATTTCCAGCTTAATCATCAGGtgatga < 1:2400026/47‑1 (MQ=255) atcaACATCCGCTATTATTGATTTCCAGCTTAATCATCAGGtgatga < 1:5343956/47‑1 (MQ=255) tcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTa < 1:3820453/68‑1 (MQ=255) tcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTa < 1:668938/68‑1 (MQ=255) tcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTa < 1:3874175/68‑1 (MQ=255) caACATCCGCTATTATTGATTTCCAGCTTAATCATCAGGtgatgatt > 1:3163030/1‑45 (MQ=38) caACATCCGCTATTATTGATTTCCAGCTTAATCATCAGGtgatgatt > 1:2305124/1‑45 (MQ=38) caACATCCGCTATTATTGATTTCCAGCTTAATCATCAGGtgatgat > 1:2352660/1‑45 (MQ=38) caACATCCGCTATTATTGATTTCCAGCTTAATCATCAGGtgatgat > 1:4738190/1‑45 (MQ=38) aaCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCa > 1:251358/1‑70 (MQ=255) aaCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCa > 1:2999398/1‑70 (MQ=255) aCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATg < 1:2588338/71‑1 (MQ=255) aCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATg < 1:3033480/71‑1 (MQ=255) aCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATg < 1:4352894/71‑1 (MQ=255) cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACa < 1:4473026/45‑1 (MQ=255) cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACa < 1:136312/45‑1 (MQ=255) cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGa < 1:5443381/71‑1 (MQ=255) cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGa < 1:3489803/71‑1 (MQ=255) aTCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATGATGACTAAACCTAATCATGAg < 1:1517073/71‑1 (MQ=255) tCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGc < 1:3389054/71‑1 (MQ=255) cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCt < 1:2387396/70‑1 (MQ=255) cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAAAAAAAATAATGACTAAACCt > 1:367754/1‑59 (MQ=255) attattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGcc > 1:4872659/1‑71 (MQ=255) attattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGcc > 1:913962/1‑71 (MQ=255) ttattGATTTCCAGCTTAATCATCACCTGATGAACaaaaa < 1:960968/40‑1 (MQ=255) ttattGATTTCCAGCTTAATCATCACCTGATGAACaaaaa < 1:1822629/40‑1 (MQ=255) ttattGATTTCCAGCTTAATCATCACCTGATGAACaaaaa < 1:1510370/40‑1 (MQ=255) ttattGATTTCCAGCTTAATCATCACCTGATGAACaaaa < 1:3387921/39‑1 (MQ=255) attGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAg > 1:4649631/1‑61 (MQ=255) attGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAg > 1:2874756/1‑61 (MQ=255) attGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCgg > 1:2862108/1‑71 (MQ=255) ttGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat < 1:3067436/41‑1 (MQ=255) ttGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat < 1:3519528/41‑1 (MQ=255) ttGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTa > 1:2498480/1‑64 (MQ=255) tGATTTCCAGCTTAATCATCACCTGATGAACAAAaataat < 1:850674/40‑1 (MQ=255) tGATTTCCAGCTTAATCATCACCTGATGAACAAAaat < 1:4554089/37‑1 (MQ=255) tGATTTCCAGCTTAATCATCACCTGATGAACAAAaat < 1:3611300/37‑1 (MQ=255) gATTTCCAGCTTAATCATCACCTGATGAACAAAaat < 1:4343840/36‑1 (MQ=255) gATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGa > 1:2749465/1‑41 (MQ=255) gATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGa > 1:4670607/1‑41 (MQ=255) tttCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTg < 1:2595394/71‑1 (MQ=255) ccAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGAt < 1:1597667/70‑1 (MQ=255) cAGCTTAATCATCACCTGATGAACAAAAATAATGa > 1:4577802/1‑35 (MQ=255) aGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTg < 1:1783044/66‑1 (MQ=255) aGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTg < 1:712592/66‑1 (MQ=255) aGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGt < 1:2212757/71‑1 (MQ=255) gCTTAATCATCACCTTATGAACAAAAATAATGACTAAACCTAATCATg < 1:2471399/48‑1 (MQ=255) gCTTAATCATCACCTGATGAACAAAAATAATGACTaa < 1:3185317/37‑1 (MQ=255) gCTTAATCATCACCTGATGAACAAAAATAATGACTaa < 1:2309917/37‑1 (MQ=255) cTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGc < 1:415865/71‑1 (MQ=255) tAATCATCACCTGATGAACAAAAATAATGACTAAAcc > 1:5491558/1‑37 (MQ=255) aaTCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCt < 1:478209/69‑1 (MQ=255) aaTCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCt < 1:1345003/69‑1 (MQ=255) aaTCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCt < 1:4618501/69‑1 (MQ=255) aaTCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGa > 1:850473/1‑71 (MQ=255) aaTCATCACCTGATGAACAAAAATAATGACTAAACCTAATAATGAGCTTAGCCCGGCGCTGATCg > 1:3986684/1‑65 (MQ=255) atcatcACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCt < 1:1496183/68‑1 (MQ=255) atcatcACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGAt < 1:2570681/71‑1 (MQ=255) atcatcACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGAt < 1:4146996/71‑1 (MQ=255) tcatcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATg < 1:3150512/71‑1 (MQ=255) catcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCt < 1:2040587/66‑1 (MQ=255) atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTc > 1:2767483/1‑71 (MQ=255) aCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCt > 1:535526/1‑69 (MQ=255) ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTa > 1:1000180/1‑42 (MQ=255) ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTa > 1:1481878/1‑42 (MQ=255) ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTa > 1:3943535/1‑42 (MQ=255) ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTa > 1:4968823/1‑42 (MQ=255) ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTa > 1:246362/1‑42 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGgcgc < 1:2734368/50‑1 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGgcgc < 1:1613394/50‑1 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGgcgc < 1:2776554/50‑1 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCg < 1:1848756/56‑1 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTg < 1:795986/58‑1 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCt < 1:2164744/60‑1 (MQ=255) cTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCt < 1:194483/67‑1 (MQ=255) | TTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCT > minE/1801395‑1801531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |