| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,817,482 | A→G | 7.1% | S10S (AGT→AGC) | ygaD ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,817,482 | 0 | A | G | 7.1% | 234.7 / 6.5 | 84 | S10S (AGT→AGC) | ygaD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (38/40); new base G (4/2); total (42/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01 | |||||||||||
CCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAG > minE/1817414‑1817552 | ccaccGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACt > 1:1945674/1‑71 (MQ=255) caccGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACtt < 1:5094713/71‑1 (MQ=255) caccGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACtt < 1:729175/71‑1 (MQ=255) gccGGTACAAGACTCAGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCGCTTa < 1:5081849/70‑1 (MQ=255) gccGGTACAAGACTCAGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCGCTTa < 1:3611963/70‑1 (MQ=255) accGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACtt > 1:2697397/1‑70 (MQ=255) gACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAg > 1:88515/1‑71 (MQ=255) gACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAg < 1:1498194/71‑1 (MQ=255) aCTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGt > 1:1589122/1‑71 (MQ=255) cTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGtt < 1:1491087/71‑1 (MQ=255) cGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTc > 1:4456877/1‑70 (MQ=255) cGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCa > 1:4585644/1‑71 (MQ=255) ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTc < 1:3638865/69‑1 (MQ=255) gCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACtt < 1:2238843/54‑1 (MQ=255) aGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgt < 1:3107937/71‑1 (MQ=255) aGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgt > 1:4917766/1‑71 (MQ=255) ttgttACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgt > 1:3768592/1‑69 (MQ=255) ttgttACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgt > 1:4164986/1‑69 (MQ=255) tACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca > 1:4634339/1‑71 (MQ=255) tACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca > 1:3772296/1‑71 (MQ=255) aCGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACt > 1:2191415/1‑44 (MQ=255) aCGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCa > 1:1362065/1‑55 (MQ=255) aCGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca < 1:4546497/70‑1 (MQ=255) aCGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca < 1:2502923/70‑1 (MQ=255) aCGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca < 1:3391856/70‑1 (MQ=255) aCGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcat > 1:1710665/1‑71 (MQ=255) cGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatc < 1:3073998/71‑1 (MQ=255) cACGTGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATc < 1:4212194/44‑1 (MQ=255) cACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATc < 1:3798727/44‑1 (MQ=255) cACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATc < 1:2288039/44‑1 (MQ=255) cACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACctct < 1:3377661/71‑1 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTaa > 1:3287898/1‑35 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGc > 1:5304894/1‑39 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgt < 1:4310777/52‑1 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACctctct > 1:1777/1‑69 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgt > 1:4535954/1‑71 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgt < 1:498416/71‑1 (MQ=255) cGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgt > 1:858791/1‑71 (MQ=255) gggCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtc < 1:4162979/71‑1 (MQ=255) gCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtcag < 1:5400440/71‑1 (MQ=255) gCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGGCAGTCATCACAACCTCTgtcag < 1:2653939/71‑1 (MQ=255) tttCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtcagt > 1:5230728/1‑70 (MQ=255) tCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtcagtca > 1:3506762/1‑70 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCAc > 1:97389/1‑40 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtca > 1:3477621/1‑64 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtca > 1:2652119/1‑64 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCaaa > 1:5544554/1‑70 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCaaa < 1:3725165/70‑1 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCaaa > 1:3508128/1‑70 (MQ=255) aGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCaaa < 1:2584842/70‑1 (MQ=255) ccTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAtt < 1:954435/70‑1 (MQ=255) cTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAtt < 1:2491278/69‑1 (MQ=255) gCCCAACCTGTTCGCTTAACTGCATCAGTTCACTGTCAGtcatca > 1:2141382/1‑45 (MQ=255) gCCCAACCTGTTCGCTTAACTGCATCAGTTCACTGTCAGtcatca > 1:1853131/1‑45 (MQ=255) gCCCAACCTGTTCGCTTAACTGCATCAGTTCACTGTCAGtcatca > 1:4597250/1‑45 (MQ=255) gCCCAACCTGTTCGCTTAACTGCATCAGTTCACTGTCAGtcatca > 1:1837279/1‑45 (MQ=255) gCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATcac < 1:481672/46‑1 (MQ=255) gCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCaaa < 1:5524255/63‑1 (MQ=255) gCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCaaa < 1:1976377/63‑1 (MQ=255) cccAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTAt < 1:4945622/69‑1 (MQ=255) cccAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGc < 1:510872/71‑1 (MQ=255) ccAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACaa > 1:2891178/1‑46 (MQ=255) ccAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACaa > 1:4413301/1‑46 (MQ=255) ccAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACaa > 1:2193565/1‑46 (MQ=255) aaCCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAtt < 1:1273244/63‑1 (MQ=255) aaCCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCg > 1:697977/1‑70 (MQ=255) aCCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAAcc < 1:2049076/45‑1 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca > 1:800129/1‑39 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca > 1:1586300/1‑39 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca > 1:3731144/1‑39 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTg < 1:88287/48‑1 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTg < 1:2215694/48‑1 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTg < 1:1169882/48‑1 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGAc < 1:187840/71‑1 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGAc < 1:5654878/71‑1 (MQ=255) ccTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGAc < 1:4138990/71‑1 (MQ=255) cTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACa < 1:3860814/71‑1 (MQ=255) tGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgtc > 1:2870180/1‑48 (MQ=255) gTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGAc > 1:3876611/1‑68 (MQ=255) ttCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGAc > 1:2317587/1‑67 (MQ=255) ttCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGa < 1:3511314/71‑1 (MQ=255) tCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAtt < 1:709476/56‑1 (MQ=255) cACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACaa > 1:117198/1‑67 (MQ=255) cACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGa > 1:3912824/1‑69 (MQ=255) aCTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAg > 1:883743/1‑71 (MQ=255) | CCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAG > minE/1817414‑1817552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |