| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,256 | G→A | 49.1% | A23V (GCG→GTG) ‡ | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,256 | 0 | G | A | 49.1% | 5.5 / 156.6 | 116 | A23V (GCG→GTG) ‡ | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base G (39/20); new base A (31/26); total (70/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
GATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAA > minE/1826192‑1826326 | gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg > 1:1488008/1‑71 (MQ=255)gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg > 1:4261569/1‑71 (MQ=255)gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACg > 1:3988531/1‑71 (MQ=255) tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTcac < 1:612773/71‑1 (MQ=255) tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGt < 1:1239893/67‑1 (MQ=255) ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACgg > 1:737801/1‑70 (MQ=255) tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTcac > 1:1112419/1‑61 (MQ=255) ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAAc > 1:5412158/1‑70 (MQ=255) ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGTCAAcc < 1:3590309/71‑1 (MQ=255) ggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa < 1:2852499/71‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACTCACGCTACTACACAGGTACGGCAACCAATACa > 1:508002/1‑71 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTa > 1:3008819/1‑57 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTa > 1:3961321/1‑57 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCaa > 1:4262611/1‑67 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCaa > 1:4462017/1‑67 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATAc < 1:3326430/70‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa > 1:1007127/1‑71 (MQ=255) gCGATGCACAGTCCTGATTTTACTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa < 1:765359/70‑1 (MQ=255) cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:4151053/71‑1 (MQ=255) cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:1007139/71‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg < 1:966424/51‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg < 1:4132488/51‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTa < 1:1973093/71‑1 (MQ=255) gCACAGTCCTGATTTTGCTGATGAGAAACCACGCACGCTACTTCACAGGTa > 1:3382647/1‑51 (MQ=38) gCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAg > 1:1436344/1‑48 (MQ=255) gCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAg > 1:4513815/1‑48 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTAc > 1:2238228/1‑51 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTAc > 1:2244745/1‑51 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc < 1:3192130/68‑1 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc < 1:4541114/68‑1 (MQ=255) aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAgg < 1:1078367/43‑1 (MQ=12) aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgca > 1:649410/3‑70 (MQ=255) aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc < 1:2550428/68‑1 (MQ=255) aGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg < 1:181518/45‑1 (MQ=255) aGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg < 1:1912060/45‑1 (MQ=255) gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg < 1:1449875/44‑1 (MQ=255) gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:4059290/1‑66 (MQ=255) gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:133643/1‑66 (MQ=255) atCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat > 1:1623890/2‑69 (MQ=255) atCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat > 1:4393609/2‑69 (MQ=255) atCCTGAATCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat < 1:1165970/70‑3 (MQ=255) tCCTGATTTTCCTTATGAGAAACCACGCACGCTACTTCACAGGTACGGc < 1:3634267/49‑1 (MQ=255) tCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc < 1:1982038/49‑1 (MQ=255) tCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACg > 1:2740211/1‑47 (MQ=16) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:880577/1‑71 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:3502090/1‑71 (MQ=255) cTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt > 1:1394768/1‑58 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa > 1:3370833/1‑43 (MQ=12) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa > 1:4594554/1‑43 (MQ=12) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa > 1:5233976/1‑43 (MQ=12) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa > 1:386318/1‑43 (MQ=12) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTa > 1:5239275/1‑43 (MQ=12) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga < 1:1103046/53‑3 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga < 1:2051226/53‑3 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAt > 1:2844205/1‑58 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAt > 1:1124460/1‑58 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAt > 1:4947987/1‑58 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:3681554/1‑61 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:3799546/1‑61 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc > 1:5298515/1‑70 (MQ=255) gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCa < 1:4354397/46‑1 (MQ=16) gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCa < 1:2131427/46‑1 (MQ=16) gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCa < 1:4620319/46‑1 (MQ=16) gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc > 1:4850430/1‑70 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCa > 1:1634268/1‑45 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:5036326/1‑71 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:3737640/1‑70 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:515577/1‑62 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:602642/69‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:1264433/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:4796910/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:3267245/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa < 1:3064599/70‑1 (MQ=255) ttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc < 1:2303942/41‑1 (MQ=255) tCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4631497/1‑71 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:5204141/69‑2 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAataa < 1:2683741/67‑3 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:1356230/1‑70 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:3092677/1‑70 (MQ=255) atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:5350741/1‑71 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa < 1:2500988/70‑1 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:4810690/1‑71 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:2564220/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:3193442/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:1061345/1‑71 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCaa > 1:1746774/1‑35 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4546756/1‑64 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:4285982/1‑71 (MQ=255) gaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt < 1:4195368/71‑1 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:1646897/71‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat < 1:846580/68‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat > 1:1878049/4‑71 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:1485272/1‑54 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:3727298/1‑54 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:363866/69‑2 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:437939/69‑2 (MQ=255) tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAat < 1:863635/53‑1 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:3596661/1‑68 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:3055161/1‑68 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACAATTCGTCGCATCCGCAATAATAAACCgg > 1:2367061/1‑63 (MQ=255) | GATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAA > minE/1826192‑1826326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |