| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,303 | A→C | 21.5% | A7A (GCT→GCG) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,303 | 0 | A | C | 21.5% | 204.4 / 78.5 | 130 | A7A (GCT→GCG) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base A (54/48); new base C (13/15); total (67/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
CTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACA > minE/1826234‑1826366 | cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:880577/1‑71 (MQ=255)cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:4335482/1‑71 (MQ=255)cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:3502090/1‑71 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc > 1:5298515/1‑70 (MQ=255) gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc > 1:4850430/1‑70 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:5036326/1‑71 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa < 1:2285167/70‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:602642/69‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:1264433/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:3267245/69‑1 (MQ=255) tCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4631497/1‑71 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCACCCGATACATTTCGTCGCATCCGCaataata > 1:4067795/2‑69 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:5204141/69‑2 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAataa < 1:2683741/67‑3 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:1356230/1‑70 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:3092677/1‑70 (MQ=255) atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:860411/1‑71 (MQ=255) atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:5350741/1‑71 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:2564220/1‑71 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:4810690/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:1061345/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:3193442/1‑71 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4546756/1‑64 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:4285982/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:4197328/1‑71 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:3715697/1‑70 (MQ=255) gaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaa > 1:113282/1‑65 (MQ=255) gaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt < 1:4195368/71‑1 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:1646897/71‑1 (MQ=255) aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg < 1:5528802/54‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat < 1:846580/68‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat > 1:1878049/4‑71 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:3727298/1‑54 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:1485272/1‑54 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:363866/69‑2 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:437939/69‑2 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata > 1:685906/1‑56 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACAATTCGTCGCATCCGCAATAATAAACCgg > 1:2367061/1‑63 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:4714803/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:788160/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:2827556/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACAATTACTCCCGTCAGCa > 1:1079576/2‑51 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:2286834/1‑59 (MQ=255) cgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaacc < 1:1726768/60‑3 (MQ=255) gcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgatg < 1:227899/55‑1 (MQ=255) gcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataataa < 1:3399526/56‑3 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:3055557/71‑1 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:4459305/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:4349942/71‑1 (MQ=255) gCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct > 1:4964328/1‑70 (MQ=255) tACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:2931810/1‑66 (MQ=255) cTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:683848/47‑2 (MQ=255) cTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:1849232/47‑2 (MQ=255) cTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:3698722/47‑2 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:2833369/1‑50 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:4611331/71‑1 (MQ=255) tCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:1485004/1‑40 (MQ=255) tCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:875849/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:1649699/1‑50 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:2277003/1‑50 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACTGTTCATCAAATTCTCCAGCAAt > 1:2634725/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:761459/1‑70 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt < 1:1570595/70‑1 (MQ=255) aGGTACGGCAACCGATACATTTCGTCGCATCCGCAat > 1:2481015/1‑37 (MQ=16) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:2881310/1‑39 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:3002386/1‑39 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:2382824/1‑39 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4438090/1‑39 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4887213/1‑39 (MQ=255) tACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:3427/71‑1 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:3380428/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:3680/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:2694846/70‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGAc < 1:2180224/65‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGAc < 1:2658611/65‑1 (MQ=255) gCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:3059631/1‑70 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATgcg > 1:5187152/1‑71 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc > 1:3609962/1‑71 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:4153070/60‑1 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGa > 1:533537/1‑71 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTAATCAAATtc < 1:379588/44‑1 (MQ=255) aCATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:3074050/1‑71 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct > 1:5587444/1‑43 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc > 1:2136589/1‑45 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:3890578/38‑1 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:3844548/1‑38 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt > 1:4324407/1‑37 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt > 1:1775688/1‑71 (MQ=255) cTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:980644/42‑1 (MQ=255) cTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:3270990/42‑1 (MQ=255) tCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:1984563/53‑1 (MQ=255) cgcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:3071083/52‑1 (MQ=255) gcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTcaca < 1:3063555/69‑1 (MQ=255) gcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTcaca < 1:3651172/69‑1 (MQ=255) gcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTcaca < 1:4505748/69‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCa < 1:1916927/37‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:5405472/48‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc < 1:1102610/54‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc < 1:4544233/54‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:1397349/58‑1 (MQ=255) | CTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACA > minE/1826234‑1826366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |