| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,315 | A→C | 7.8% | R3R (CGT→CGG) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,315 | 0 | A | C | 7.8% | 261.2 / 14.8 | 103 | R3R (CGT→CGG) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base A (46/49); new base C (4/4); total (50/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
ATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTT > minE/1826246‑1826382 | atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:5350741/1‑71 (MQ=255)atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:860411/1‑71 (MQ=255)atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa < 1:2500988/70‑1 (MQ=255)atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:2564220/1‑71 (MQ=255)atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:4810690/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:1061345/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:3193442/1‑71 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:4285982/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:4197328/1‑71 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:3715697/1‑70 (MQ=255) gaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt < 1:4195368/71‑1 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:1646897/71‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat < 1:846580/68‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat > 1:1878049/4‑71 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:363866/69‑2 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:3596661/1‑68 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:3055161/1‑68 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACAATTCGTCGCATCCGCAATAATAAACCgg > 1:2367061/1‑63 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:4714803/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:788160/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:2827556/2‑70 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:2286834/1‑59 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:4459305/71‑1 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:3055557/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:4349942/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:1406533/71‑1 (MQ=255) tACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:2931810/1‑66 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:2833369/1‑50 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:4611331/71‑1 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc < 1:780530/50‑1 (MQ=255) tCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:875849/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:2277003/1‑50 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACTGTTCATCAAATTCTCCAGCAAt > 1:2634725/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:761459/1‑70 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:4893344/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:3200815/1‑71 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCGAGCAAt < 1:4551119/70‑1 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt < 1:1570595/70‑1 (MQ=255) tACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:1846860/71‑1 (MQ=255) tACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:3427/71‑1 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:3380428/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:3680/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:3899912/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:2694846/70‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGAc < 1:266824/65‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGAc < 1:2658611/65‑1 (MQ=255) gCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:3059631/1‑70 (MQ=255) gCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:1607714/1‑70 (MQ=255) gCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:3394854/1‑70 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATgcg > 1:5187152/1‑71 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc > 1:3609962/1‑71 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:4153070/60‑1 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGa > 1:533537/1‑71 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGa > 1:5391877/1‑71 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTAATCAAATtc < 1:379588/44‑1 (MQ=255) aCATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:3074050/1‑71 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct > 1:5587444/1‑43 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc > 1:2136589/1‑45 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:3890578/38‑1 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:4338875/38‑1 (MQ=255) aTTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:1935836/38‑1 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:3844548/1‑38 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt > 1:4324407/1‑37 (MQ=255) ttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt > 1:1775688/1‑71 (MQ=255) cTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:980644/42‑1 (MQ=255) tCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:1984563/53‑1 (MQ=255) tCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:228687/53‑1 (MQ=255) cgcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:3071083/52‑1 (MQ=255) gcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTcaca < 1:3063555/69‑1 (MQ=255) gcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTcaca < 1:4505748/69‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCa < 1:1916927/37‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa < 1:5405472/48‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc < 1:4544233/54‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc < 1:1102610/54‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:1397349/58‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:5242618/58‑1 (MQ=255) tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:3686111/58‑1 (MQ=255) atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAg < 1:2140160/71‑1 (MQ=255) tgatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTa < 1:2309971/40‑1 (MQ=255) tgatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTa < 1:5614095/40‑1 (MQ=255) tgatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:1178277/51‑1 (MQ=255) tgatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:3464499/51‑1 (MQ=255) tgatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGt < 1:2305325/71‑1 (MQ=255) atgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:4153284/1‑39 (MQ=255) atgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATTCGCCGATACATTCACAAATCa > 1:130738/1‑62 (MQ=255) atgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATc > 1:3472620/1‑61 (MQ=255) atgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGttt > 1:3257437/1‑71 (MQ=255) atgaAACGGTTCATCAAATTCTCCAGCAATGAAAGt > 1:4018804/1‑36 (MQ=255) tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGtttt < 1:875600/71‑1 (MQ=255) tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGtttt > 1:439443/1‑71 (MQ=255) tgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGtttt > 1:1793718/1‑71 (MQ=255) gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGcc > 1:96935/1‑64 (MQ=255) gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGcc > 1:4317757/1‑64 (MQ=255) gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGcc > 1:2679566/1‑64 (MQ=255) gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGcc > 1:2916635/1‑64 (MQ=255) aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACa > 1:3546946/1‑48 (MQ=255) aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACa > 1:3843278/1‑48 (MQ=255) aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACa > 1:1137824/1‑48 (MQ=255) aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGttttt > 1:2400256/1‑70 (MQ=255) aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGttttt > 1:5298949/1‑70 (MQ=255) | ATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTT > minE/1826246‑1826382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |