| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,837,432 | G→A | 12.4% | N329N (AAC→AAT) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,837,432 | 0 | G | A | 12.4% | 271.3 / 23.0 | 105 | N329N (AAC→AAT) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (45/47); new base A (9/4); total (54/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCG > minE/1837363‑1837502 | ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGGATTGTGTCGt > 1:3249609/1‑71 (MQ=255)ggTGCTACCCATGGTCGCTATTTTTTGCCCAGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:2943337/71‑1 (MQ=255) gTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt < 1:897355/71‑1 (MQ=255) tACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa < 1:648261/68‑1 (MQ=255) ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:5222695/1‑65 (MQ=255) ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCg < 1:5219593/71‑1 (MQ=255) gTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAgg < 1:3042154/70‑1 (MQ=255) tCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATgg < 1:909798/62‑1 (MQ=255) cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa < 1:2993430/56‑1 (MQ=255) cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa < 1:2625171/56‑1 (MQ=255) aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:593596/1‑71 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt < 1:3075263/60‑1 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCa > 1:951753/1‑65 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg > 1:935329/1‑71 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg > 1:2161948/1‑71 (MQ=255) ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:4807759/1‑51 (MQ=255) ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:4668441/1‑51 (MQ=255) ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:5074047/1‑51 (MQ=255) ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt > 1:725762/1‑70 (MQ=255) ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt > 1:3680270/1‑70 (MQ=255) tttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt > 1:3164911/1‑69 (MQ=255) ttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa < 1:3033293/70‑1 (MQ=255) cccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:2421201/1‑45 (MQ=255) cccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:883277/1‑45 (MQ=255) ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat < 1:2586968/69‑1 (MQ=255) ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat < 1:2746298/69‑1 (MQ=255) cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:577200/1‑71 (MQ=255) cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:3523343/1‑71 (MQ=255) cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:1830435/1‑71 (MQ=255) gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCga > 1:5524239/1‑39 (MQ=255) gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:4166284/1‑70 (MQ=255) gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:1943963/1‑70 (MQ=255) ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa > 1:1506986/1‑71 (MQ=255) cTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt > 1:3004534/1‑71 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat < 1:3249820/64‑1 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa > 1:2614351/1‑57 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa > 1:5319443/1‑57 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGGATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:2500831/71‑1 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:2222326/71‑1 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:5594504/71‑1 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:3627885/71‑1 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:5127570/71‑1 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt < 1:877407/70‑1 (MQ=255) cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt < 1:2011674/69‑1 (MQ=255) cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt < 1:254132/69‑1 (MQ=255) cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt < 1:3885381/69‑1 (MQ=255) ttgttgTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa < 1:4508513/51‑1 (MQ=255) tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga > 1:3877715/1‑70 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:4754187/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:5424680/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:4205122/70‑1 (MQ=255) ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa < 1:343340/70‑1 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:3393886/1‑64 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc < 1:470958/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc < 1:1129091/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:4905618/71‑1 (MQ=255) cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCa > 1:1274032/1‑39 (MQ=255) cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2303730/1‑57 (MQ=255) tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt < 1:216349/67‑1 (MQ=255) tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt < 1:940574/67‑1 (MQ=255) cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt < 1:2104168/71‑1 (MQ=255) cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt < 1:918072/47‑1 (MQ=255) cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt < 1:210467/71‑1 (MQ=255) aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt < 1:2273518/70‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:3298963/1‑47 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:4194240/1‑47 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:4418962/1‑47 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:1609507/1‑47 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:5178212/55‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:3767932/55‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg < 1:969777/71‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg < 1:4288213/71‑1 (MQ=255) gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg > 1:4278880/1‑71 (MQ=255) cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc < 1:169398/71‑1 (MQ=255) cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc > 1:5110646/1‑71 (MQ=255) atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc > 1:2584178/4‑70 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa < 1:4052296/41‑1 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa < 1:398584/41‑1 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:1587023/1‑49 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:2007803/1‑65 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg > 1:1663468/1‑68 (MQ=255) ttGTGTAGTTATGGGCGTAGGCACTCAGGTAATCATCATTa < 1:503388/41‑1 (MQ=255) tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc > 1:4724543/1‑71 (MQ=255) tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg > 1:4044360/1‑70 (MQ=255) tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg > 1:5221032/1‑64 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg > 1:1290134/1‑71 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg > 1:3937281/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac < 1:3115612/70‑3 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg > 1:865233/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg > 1:2447017/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg > 1:3550347/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg > 1:5366931/1‑71 (MQ=255) tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:744114/1‑56 (MQ=255) tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:3683978/1‑56 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtga > 1:5143050/1‑43 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg < 1:5070809/53‑1 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTAt < 1:2391443/35‑1 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg > 1:279509/1‑71 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg > 1:171463/1‑71 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga > 1:2485721/1‑69 (MQ=255) | GGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCG > minE/1837363‑1837502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |