| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,837,474 | G→C | 36.7% | I315M (ATC→ATG) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,837,474 | 0 | G | C | 36.7% | 66.1 / 75.2 | 79 | I315M (ATC→ATG) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (28/22); new base C (17/12); total (45/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
TGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAA > minE/1837406‑1837544 | tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga > 1:3877715/1‑70 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:5424680/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:4754187/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:4205122/70‑1 (MQ=255) ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa < 1:343340/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc < 1:1129091/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc < 1:470958/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:4905618/71‑1 (MQ=255) tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt < 1:940574/67‑1 (MQ=255) tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt < 1:216349/67‑1 (MQ=255) cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt < 1:2104168/71‑1 (MQ=255) cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt < 1:210467/71‑1 (MQ=255) aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt < 1:2273518/70‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:3767932/55‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:5178212/55‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:760127/55‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg < 1:4288213/71‑1 (MQ=255) gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg < 1:969777/71‑1 (MQ=255) gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg > 1:4278880/1‑71 (MQ=255) cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc < 1:169398/71‑1 (MQ=255) cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc > 1:5110646/1‑71 (MQ=255) atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc > 1:2584178/4‑70 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:2007803/1‑65 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg > 1:1663468/1‑68 (MQ=255) tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc > 1:4724543/1‑71 (MQ=255) tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg > 1:4044360/1‑70 (MQ=255) tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg > 1:5221032/1‑64 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg > 1:3937281/1‑71 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg > 1:1290134/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac < 1:3115612/70‑3 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg > 1:2447017/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg > 1:865233/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg > 1:3550347/1‑71 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg > 1:5366931/1‑71 (MQ=255) tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:744114/1‑56 (MQ=255) tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:3683978/1‑56 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg > 1:832223/4‑63 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa < 1:4151842/67‑1 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa < 1:147037/67‑1 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg > 1:4608661/4‑71 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg > 1:4552884/4‑71 (MQ=255) tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg > 1:435145/4‑71 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg < 1:5070809/53‑1 (MQ=255) cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc > 1:1162839/3‑58 (MQ=255) cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg > 1:5488917/3‑70 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg > 1:279509/1‑71 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg > 1:171463/1‑71 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga > 1:2485721/1‑69 (MQ=255) attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt > 1:2727593/2‑53 (MQ=255) attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc > 1:3314409/2‑57 (MQ=255) attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac < 1:2958374/64‑3 (MQ=255) attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct < 1:4579251/70‑2 (MQ=255) ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg < 1:2302439/71‑3 (MQ=255) ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg < 1:4749363/71‑3 (MQ=255) ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg < 1:5610259/71‑3 (MQ=255) taaTGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa < 1:1184967/65‑1 (MQ=255) tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc > 1:4100491/1‑70 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTTTGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt > 1:1414191/1‑70 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt < 1:2870711/71‑1 (MQ=255) gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa > 1:3229625/1‑42 (MQ=255) gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt > 1:4294273/1‑43 (MQ=255) gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt > 1:4626672/1‑43 (MQ=255) gCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat < 1:1852372/38‑1 (MQ=255) tAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:1068833/40‑1 (MQ=255) ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa > 1:2287529/1‑70 (MQ=255) gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:1831448/71‑1 (MQ=255) cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc < 1:4423282/62‑1 (MQ=255) cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac < 1:2100593/71‑1 (MQ=255) cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa > 1:4076349/1‑71 (MQ=255) ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg < 1:1838597/41‑1 (MQ=255) ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg > 1:1033989/1‑71 (MQ=255) gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:893411/63‑1 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac > 1:775615/1‑64 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac > 1:5385861/1‑64 (MQ=255) aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac > 1:2230364/1‑64 (MQ=255) catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg > 1:3969487/1‑43 (MQ=255) catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg > 1:3919408/1‑43 (MQ=255) catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg > 1:2394416/1‑43 (MQ=255) catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac < 1:1029486/61‑1 (MQ=255) atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa > 1:2835029/1‑62 (MQ=255) atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAt < 1:43273/71‑1 (MQ=255) atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt < 1:5115252/70‑1 (MQ=255) atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt < 1:5096701/70‑1 (MQ=255) ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa > 1:4966377/1‑55 (MQ=255) tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa > 1:4051478/1‑55 (MQ=255) gTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTc > 1:2657380/1‑71 (MQ=255) tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa < 1:4623498/40‑1 (MQ=255) tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc > 1:2486644/1‑40 (MQ=255) tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc > 1:3657265/1‑40 (MQ=255) tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAg > 1:1107523/1‑42 (MQ=255) tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTcgc < 1:4085857/70‑1 (MQ=255) atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTAc > 1:2184482/1‑37 (MQ=255) atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCg < 1:5142663/63‑1 (MQ=255) atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt < 1:4127558/71‑1 (MQ=255) atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt < 1:1269571/71‑1 (MQ=255) tgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt < 1:2406421/70‑1 (MQ=255) gatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATaa > 1:2650116/1‑71 (MQ=255) | TGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAA > minE/1837406‑1837544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |